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- W2779278315 abstract "Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including ß-carotene, Α-carotene, and ß-cryptoxanthin. In this study, we report the successful overexpression of the IbOr gene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose of improving ß-carotene in maize. The results showed that the total carotenoid and ß-carotene content of all analyzed transgenic maize plants were significantly higher than those of wild-type lines. For H145-IbOr transgenic maize, in the best line (H145-IbOr.10), the total carotenoid and ß-carotene contents were increased up to 10.36- and 15.11-fold, respectively, compared to the wild type (H145-WT). In the case of H95-IbOr transgenic plants, 5.58-fold increase in total carotenoid and 7.63-fold increase in ß-carotene were achieved in the H95-IbOr.6 line compared to nontransgenic plants (H95-WT). In all the transgenic plants derived from the wild-type maize line with less carotenoid content (H145-WT), the content of both total carotenoid and ß-carotene was higher than in transgenic plants derived from the wild-type maize line having more carotenoid content (H95-WT). Our research is the first in successful overexpression of IbOr gene in maize." @default.
- W2779278315 created "2018-01-05" @default.
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- W2779278315 date "2017-01-01" @default.
- W2779278315 modified "2023-09-25" @default.
- W2779278315 title "Overexpression of the IbOr gene from sweet potato (Ipomea batatas ‘Hoang Long’)in maize increases total carotenoid and β-carotene contents" @default.
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- W2779278315 doi "https://doi.org/10.3906/biy-1708-87" @default.
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