FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2779844556> ?p ?o ?g. }

• W2779844556 endingPage "54" @default.
• W2779844556 startingPage "46" @default.
• W2779844556 abstract "L-Ascorbate (L-Asc), but not D-isoascorbate (D-Asc) and N-acetylcysteine (NAC) suppress HIF1 ODD-luc reporter activation induced by various inhibitors of HIF prolyl hydroxylase (PHD). The efficiency of suppression by L-Asc was sensitive to the nature of HIF PHD inhibitor chosen for reporter activation. In particular, the inhibitors developed to compete with alpha-ketoglutarate (αKG), were less sensitive to suppression by the physiological range of L-Asc (40-100 μM) than those having a strong iron chelation motif. Challenging those HIF activators in the reporter system with D-Asc demonstrated that the D-isomer, despite exhibiting the same reducing potency with respect to ferric iron, had almost no effect compared to L-Asc. Similarly, no effect on reporter activation was observed with cell-permeable reducing agent NAC up to 1 mM. Docking of L-Asc and D-Asc acid into the HIF PHD2 crystal structure showed interference of Tyr310 with respect to D-Asc. This suggests that L-Asc is not merely a reducing agent preventing enzyme inactivation. Rather, the overall results identify L-Asc as a co-substrate of HIF PHD that may compete for the binding site of αKG in the enzyme active center. This conclusion is in agreement with the results obtained recently in cell-based systems for TET enzymes and jumonji histone demethylases, where L-Asc has been proposed to act as a co-substrate and not as a reducing agent preventing enzyme inactivation." @default.
• W2779844556 created "2018-01-05" @default.
• W2779844556 creator A5015079783 @default.
• W2779844556 creator A5019026894 @default.
• W2779844556 creator A5049346822 @default.
• W2779844556 creator A5055335402 @default.
• W2779844556 creator A5067421024 @default.
• W2779844556 creator A5071412130 @default.
• W2779844556 creator A5075635066 @default.
• W2779844556 creator A5077164114 @default.
• W2779844556 creator A5079751092 @default.
• W2779844556 creator A5084347593 @default.
• W2779844556 creator A5090460839 @default.
• W2779844556 creator A5091468884 @default.
• W2779844556 creator A5091693880 @default.
• W2779844556 date "2018-04-01" @default.
• W2779844556 modified "2023-09-27" @default.
• W2779844556 title "L-ascorbic acid: A true substrate for HIF prolyl hydroxylase?" @default.
• W2779844556 cites W1496496869 @default.
• W2779844556 cites W1520292841 @default.
• W2779844556 cites W1590805502 @default.
• W2779844556 cites W1602815497 @default.
• W2779844556 cites W1964918855 @default.
• W2779844556 cites W1967314547 @default.
• W2779844556 cites W1978548672 @default.
• W2779844556 cites W2006936147 @default.
• W2779844556 cites W2010756713 @default.
• W2779844556 cites W2014202252 @default.
• W2779844556 cites W2014868633 @default.
• W2779844556 cites W2022826738 @default.
• W2779844556 cites W2023765801 @default.
• W2779844556 cites W2026462699 @default.
• W2779844556 cites W2042216712 @default.
• W2779844556 cites W2058045672 @default.
• W2779844556 cites W2062158131 @default.
• W2779844556 cites W2067077699 @default.
• W2779844556 cites W2068842128 @default.
• W2779844556 cites W2069843650 @default.
• W2779844556 cites W2069932077 @default.
• W2779844556 cites W2077954885 @default.
• W2779844556 cites W2083456009 @default.
• W2779844556 cites W2083921861 @default.
• W2779844556 cites W2085010893 @default.
• W2779844556 cites W2107277218 @default.
• W2779844556 cites W2112018328 @default.
• W2779844556 cites W2119577530 @default.
• W2779844556 cites W2129096742 @default.
• W2779844556 cites W2129400442 @default.
• W2779844556 cites W2130612841 @default.
• W2779844556 cites W2132262459 @default.
• W2779844556 cites W2139847043 @default.
• W2779844556 cites W2141776504 @default.
• W2779844556 cites W2143182501 @default.
• W2779844556 cites W2155772277 @default.
• W2779844556 cites W2157653515 @default.
• W2779844556 cites W2159099334 @default.
• W2779844556 cites W2160026651 @default.
• W2779844556 cites W2286463470 @default.
• W2779844556 cites W2290683813 @default.
• W2779844556 cites W2332542469 @default.
• W2779844556 cites W2403625569 @default.
• W2779844556 cites W1964184380 @default.
• W2779844556 doi "https://doi.org/10.1016/j.biochi.2017.12.011" @default.
• W2779844556 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6460286" @default.
• W2779844556 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29289682" @default.
• W2779844556 hasPublicationYear "2018" @default.
• W2779844556 type Work @default.
• W2779844556 sameAs 2779844556 @default.
• W2779844556 citedByCount "18" @default.
• W2779844556 countsByYear W27798445562019 @default.
• W2779844556 countsByYear W27798445562020 @default.
• W2779844556 countsByYear W27798445562021 @default.
• W2779844556 countsByYear W27798445562022 @default.
• W2779844556 countsByYear W27798445562023 @default.
• W2779844556 crossrefType "journal-article" @default.
• W2779844556 hasAuthorship W2779844556A5015079783 @default.
• W2779844556 hasAuthorship W2779844556A5019026894 @default.
• W2779844556 hasAuthorship W2779844556A5049346822 @default.
• W2779844556 hasAuthorship W2779844556A5055335402 @default.
• W2779844556 hasAuthorship W2779844556A5067421024 @default.
• W2779844556 hasAuthorship W2779844556A5071412130 @default.
• W2779844556 hasAuthorship W2779844556A5075635066 @default.
• W2779844556 hasAuthorship W2779844556A5077164114 @default.
• W2779844556 hasAuthorship W2779844556A5079751092 @default.
• W2779844556 hasAuthorship W2779844556A5084347593 @default.
• W2779844556 hasAuthorship W2779844556A5090460839 @default.
• W2779844556 hasAuthorship W2779844556A5091468884 @default.
• W2779844556 hasAuthorship W2779844556A5091693880 @default.
• W2779844556 hasBestOaLocation W27798445562 @default.
• W2779844556 hasConcept C104317684 @default.
• W2779844556 hasConcept C150194340 @default.
• W2779844556 hasConcept C159110408 @default.
• W2779844556 hasConcept C161733203 @default.
• W2779844556 hasConcept C178790620 @default.
• W2779844556 hasConcept C181199279 @default.
• W2779844556 hasConcept C185592680 @default.
• W2779844556 hasConcept C186423591 @default.
• W2779844556 hasConcept C18903297 @default.

• next