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• W2779893457 abstract "Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types (INTACT). As nuclear RNA is low in polyadenylated transcripts and conventional pulldown methods would not capture non-polyadenylated pre-mRNA, we also present our method to remove ribosomal RNA from the total nuclear RNA in preparation for nuclear RNA-Seq." @default.
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• W2779893457 date "2018-01-01" @default.
• W2779893457 modified "2023-10-13" @default.
• W2779893457 title "Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq" @default.
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• W2779893457 doi "https://doi.org/10.21769/bioprotoc.2458" @default.
• W2779893457 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8275268" @default.
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