Matches in SemOpenAlex for { <https://semopenalex.org/work/W2781424348> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2781424348 abstract "This study aimed to identify DNA of human origin and determine human sex from bloodstains using duplex PCR analysis. Two pairs of primers amplifying the Amelogenin gene, AMEL(1) and AMEL(2) and a primer pair targeting the Sex Determining Region Y gene [SRY] were compared at the preliminary stage to measure the accuracy of sex determination. The human specific region of the Cytochrome b (Cyt b) gene, sized 412 bp, successfully targeted only human-origin DNA, while other types of animal DNA (dog, cat, cow, chicken and pig) did not produce any PCR product. The SRY primer showed 100% accuracy for male identification with a 197 bp amplicon. Therefore, the SRY primer pair was chosen for sex identification. The optimal annealing temperature for duplex PCR analysis was 55 °C, producing the most distinctive PCR products, determining the male gender with 197 bp and 412 bp bands and female with only 412 bp. For sensitivity, the smallest blood sample that could be detected was 20 ml. For specificity, both human origin and sex determination could be detected in all ratios of mixed human and dog bloods, including 100 fold less human blood to animal blood. Determination of male blood in mixed male and female blood samples was also investigated. The results showed that the presence male blood could be determined in mixed female blood sample at ratios of less than 10 fold. This study demonstrated that duplex PCR analysis of Cyt b and SRY is a reliable tool to investigate human DNA and sex of questioned bloodstains." @default.
- W2781424348 created "2018-01-05" @default.
- W2781424348 creator A5012020348 @default.
- W2781424348 creator A5016150231 @default.
- W2781424348 creator A5031197588 @default.
- W2781424348 creator A5074616600 @default.
- W2781424348 date "2017-12-29" @default.
- W2781424348 modified "2023-09-27" @default.
- W2781424348 title "Human DNA Identification and Sex Determination from Bloodstains using Duplex PCR Analysis" @default.
- W2781424348 hasPublicationYear "2017" @default.
- W2781424348 type Work @default.
- W2781424348 sameAs 2781424348 @default.
- W2781424348 citedByCount "0" @default.
- W2781424348 crossrefType "journal-article" @default.
- W2781424348 hasAuthorship W2781424348A5012020348 @default.
- W2781424348 hasAuthorship W2781424348A5016150231 @default.
- W2781424348 hasAuthorship W2781424348A5031197588 @default.
- W2781424348 hasAuthorship W2781424348A5074616600 @default.
- W2781424348 hasConcept C104317684 @default.
- W2781424348 hasConcept C106434640 @default.
- W2781424348 hasConcept C153911025 @default.
- W2781424348 hasConcept C178790620 @default.
- W2781424348 hasConcept C185592680 @default.
- W2781424348 hasConcept C2777563447 @default.
- W2781424348 hasConcept C2780246931 @default.
- W2781424348 hasConcept C48023723 @default.
- W2781424348 hasConcept C49105822 @default.
- W2781424348 hasConcept C52001828 @default.
- W2781424348 hasConcept C54355233 @default.
- W2781424348 hasConcept C552990157 @default.
- W2781424348 hasConcept C73456138 @default.
- W2781424348 hasConcept C8185291 @default.
- W2781424348 hasConcept C86803240 @default.
- W2781424348 hasConcept C90583042 @default.
- W2781424348 hasConceptScore W2781424348C104317684 @default.
- W2781424348 hasConceptScore W2781424348C106434640 @default.
- W2781424348 hasConceptScore W2781424348C153911025 @default.
- W2781424348 hasConceptScore W2781424348C178790620 @default.
- W2781424348 hasConceptScore W2781424348C185592680 @default.
- W2781424348 hasConceptScore W2781424348C2777563447 @default.
- W2781424348 hasConceptScore W2781424348C2780246931 @default.
- W2781424348 hasConceptScore W2781424348C48023723 @default.
- W2781424348 hasConceptScore W2781424348C49105822 @default.
- W2781424348 hasConceptScore W2781424348C52001828 @default.
- W2781424348 hasConceptScore W2781424348C54355233 @default.
- W2781424348 hasConceptScore W2781424348C552990157 @default.
- W2781424348 hasConceptScore W2781424348C73456138 @default.
- W2781424348 hasConceptScore W2781424348C8185291 @default.
- W2781424348 hasConceptScore W2781424348C86803240 @default.
- W2781424348 hasConceptScore W2781424348C90583042 @default.
- W2781424348 hasIssue "4" @default.
- W2781424348 hasLocation W27814243481 @default.
- W2781424348 hasOpenAccess W2781424348 @default.
- W2781424348 hasPrimaryLocation W27814243481 @default.
- W2781424348 hasRelatedWork W187991543 @default.
- W2781424348 hasRelatedWork W1983884075 @default.
- W2781424348 hasRelatedWork W2000559642 @default.
- W2781424348 hasRelatedWork W2029489344 @default.
- W2781424348 hasRelatedWork W2056640198 @default.
- W2781424348 hasRelatedWork W2107197233 @default.
- W2781424348 hasRelatedWork W2126336605 @default.
- W2781424348 hasRelatedWork W2132598719 @default.
- W2781424348 hasRelatedWork W2195323259 @default.
- W2781424348 hasRelatedWork W2347685998 @default.
- W2781424348 hasRelatedWork W2349100731 @default.
- W2781424348 hasRelatedWork W2361155159 @default.
- W2781424348 hasRelatedWork W2373644456 @default.
- W2781424348 hasRelatedWork W2378105211 @default.
- W2781424348 hasRelatedWork W2379985977 @default.
- W2781424348 hasRelatedWork W2393469156 @default.
- W2781424348 hasRelatedWork W2437477983 @default.
- W2781424348 hasRelatedWork W2443080936 @default.
- W2781424348 hasRelatedWork W2766862793 @default.
- W2781424348 hasRelatedWork W3046814529 @default.
- W2781424348 hasVolume "22" @default.
- W2781424348 isParatext "false" @default.
- W2781424348 isRetracted "false" @default.
- W2781424348 magId "2781424348" @default.
- W2781424348 workType "article" @default.