Matches in SemOpenAlex for { <https://semopenalex.org/work/W2781773832> ?p ?o ?g. }
- W2781773832 abstract "The tropical bont tick, Amblyomma variegatum, is a tick species of veterinary importance and is considered as one of major pest of ruminants in Africa and in the Caribbean. It causes direct skin lesions, transmits heartwater, and reactivates bovine dermatophilosis. Tick saliva is reported to affect overall host responses through immunomodulatory and anti-inflammatory molecules, among other bioactive molecules. The general objective of this study was to better understand the role of saliva in interaction between the Amblyomma tick and the host using cellular biology approaches and proteomics, and to discuss its impact on disease transmission and/or activation. We first focused on the immuno-modulating effects of semi-fed A. variegatum female saliva on bovine peripheral blood mononuclear cells (PBMC) and monocyte-derived macrophages in vitro. We analyzed its immuno-suppressive properties by measuring the effect of saliva on PBMC proliferation, and observed a significant decrease in ConA-stimulated PBMC lymphoproliferation. We then studied the effect of saliva on bovine macrophages using flow cytometry to analyze the expression of MHC-II and co-stimulation molecules (CD40, CD80, and CD86) and by measuring the production of nitric oxide (NO) and pro- or anti-inflammatory cytokines. We observed a significant decrease in the expression of MHC-II, CD40, and CD80 molecules, associated with decreased levels of IL-12-p40 and TNF-α and increased level of IL-10, which could explain the saliva-induced modulation of NO. To elucidate these immunomodulatory effects, crude saliva proteins were analyzed using proteomics with an Orbitrap Elite mass spectrometer. Among the 336 proteins identified in A. variegatum saliva, we evidenced bioactive molecules exhibiting anti-inflammatory, immuno-modulatory, and anti-oxidant properties (e.g., serpins, phospholipases A2, heme lipoprotein). We also characterized an intriguing ubiquitination complex that could be involved in saliva-induced immune modulation of the host. We propose a model for the interaction between A. variegatum saliva and host immune cells that could have an effect during tick feeding by favoring pathogen dissemination or activation by reducing the efficiency of host immune response to the corresponding tick-borne diseases." @default.
- W2781773832 created "2018-01-12" @default.
- W2781773832 creator A5004329591 @default.
- W2781773832 creator A5014046255 @default.
- W2781773832 creator A5016661855 @default.
- W2781773832 creator A5018813529 @default.
- W2781773832 creator A5018861809 @default.
- W2781773832 creator A5026923535 @default.
- W2781773832 creator A5034877650 @default.
- W2781773832 creator A5037937273 @default.
- W2781773832 creator A5057831849 @default.
- W2781773832 creator A5060764244 @default.
- W2781773832 creator A5060771518 @default.
- W2781773832 creator A5065183388 @default.
- W2781773832 creator A5079517031 @default.
- W2781773832 creator A5085088838 @default.
- W2781773832 date "2018-01-04" @default.
- W2781773832 modified "2023-10-17" @default.
- W2781773832 title "Immunomodulatory Effects of Amblyomma variegatum Saliva on Bovine Cells: Characterization of Cellular Responses and Identification of Molecular Determinants" @default.
- W2781773832 cites W12339623 @default.
- W2781773832 cites W1535820401 @default.
- W2781773832 cites W1543598035 @default.
- W2781773832 cites W1558689228 @default.
- W2781773832 cites W1693967370 @default.
- W2781773832 cites W1826923758 @default.
- W2781773832 cites W1895914524 @default.
- W2781773832 cites W1919729533 @default.
- W2781773832 cites W1963891097 @default.
- W2781773832 cites W1966047761 @default.
- W2781773832 cites W1966941686 @default.
- W2781773832 cites W1968427039 @default.
- W2781773832 cites W1968768356 @default.
- W2781773832 cites W1980995798 @default.
- W2781773832 cites W1982440565 @default.
- W2781773832 cites W1986701745 @default.
- W2781773832 cites W1986989445 @default.
- W2781773832 cites W1988738406 @default.
- W2781773832 cites W1990702040 @default.
- W2781773832 cites W199620005 @default.
- W2781773832 cites W2000750335 @default.
- W2781773832 cites W2003386935 @default.
- W2781773832 cites W2004095269 @default.
- W2781773832 cites W2004756198 @default.
- W2781773832 cites W2006022163 @default.
- W2781773832 cites W2006385264 @default.
- W2781773832 cites W2008978967 @default.
- W2781773832 cites W2012540162 @default.
- W2781773832 cites W2014468157 @default.
- W2781773832 cites W2016242814 @default.
- W2781773832 cites W2022370671 @default.
- W2781773832 cites W2030266323 @default.
- W2781773832 cites W2031134923 @default.
- W2781773832 cites W2036321220 @default.
- W2781773832 cites W2036474409 @default.
- W2781773832 cites W2041995177 @default.
- W2781773832 cites W2042411401 @default.
- W2781773832 cites W2055127625 @default.
- W2781773832 cites W2057761395 @default.
- W2781773832 cites W2065981962 @default.
- W2781773832 cites W2071588176 @default.
- W2781773832 cites W2075774945 @default.
- W2781773832 cites W2080752012 @default.
- W2781773832 cites W2080983733 @default.
- W2781773832 cites W2092172266 @default.
- W2781773832 cites W2093316664 @default.
- W2781773832 cites W2094810051 @default.
- W2781773832 cites W2101961230 @default.
- W2781773832 cites W2103210188 @default.
- W2781773832 cites W2105773805 @default.
- W2781773832 cites W2109965008 @default.
- W2781773832 cites W2115673680 @default.
- W2781773832 cites W2115681156 @default.
- W2781773832 cites W2120112111 @default.
- W2781773832 cites W2124352754 @default.
- W2781773832 cites W2125526836 @default.
- W2781773832 cites W2129084302 @default.
- W2781773832 cites W2130925474 @default.
- W2781773832 cites W2134749398 @default.
- W2781773832 cites W2134789671 @default.
- W2781773832 cites W2135290399 @default.
- W2781773832 cites W2144652377 @default.
- W2781773832 cites W2147973026 @default.
- W2781773832 cites W2149200346 @default.
- W2781773832 cites W2149694557 @default.
- W2781773832 cites W2154344000 @default.
- W2781773832 cites W216142094 @default.
- W2781773832 cites W2171480404 @default.
- W2781773832 cites W2259419555 @default.
- W2781773832 cites W2265652283 @default.
- W2781773832 cites W2285153862 @default.
- W2781773832 cites W2296062525 @default.
- W2781773832 cites W2309366384 @default.
- W2781773832 cites W2314997748 @default.
- W2781773832 cites W2332994595 @default.
- W2781773832 cites W2339416841 @default.
- W2781773832 cites W2345935592 @default.
- W2781773832 cites W2377781519 @default.
- W2781773832 cites W2509040514 @default.
- W2781773832 cites W2617076055 @default.
- W2781773832 cites W2635677524 @default.