Matches in SemOpenAlex for { <https://semopenalex.org/work/W2782104271> ?p ?o ?g. }
- W2782104271 endingPage "1291" @default.
- W2782104271 startingPage "1281" @default.
- W2782104271 abstract "E. coli AlkB, a repair enzyme of the dioxygenase family, catalyses the removal of mutagenic methylated nucleotides from the genome. Known for substrate promiscuity, AlkB's catalytic mechanism and conformational changes accompanying substrate binding have been extensively dissected. However, the structural parameters of various substrates governing their recognition by AlkB still remain elusive. In this work, through solution-state vibrational spectra of methylated substrates bound to AlkB in combination with computational analysis, we show that the recognition specificity is dictated by the protonation states of the substrates. Specificity is conferred predominantly through hydrogen bonding and cation-π interactions. Furthermore, we report on the interaction of AlkB with normal, unmodified nucleotides, wherein the presence of an exocyclic amino group serves as an essential criterion for the initial process of substrate recognition. Taken together, these results provide a rationale for structural determinants of substrate specificity as well as mode of lesion discrimination employed by AlkB." @default.
- W2782104271 created "2018-01-12" @default.
- W2782104271 creator A5002509159 @default.
- W2782104271 creator A5035705840 @default.
- W2782104271 creator A5065013390 @default.
- W2782104271 creator A5084805246 @default.
- W2782104271 date "2018-01-01" @default.
- W2782104271 modified "2023-09-27" @default.
- W2782104271 title "Structural basis for substrate discrimination by<i>E. coli</i>repair enzyme, AlkB" @default.
- W2782104271 cites W105839754 @default.
- W2782104271 cites W1510319360 @default.
- W2782104271 cites W1537176556 @default.
- W2782104271 cites W1965547460 @default.
- W2782104271 cites W1978744896 @default.
- W2782104271 cites W1984516599 @default.
- W2782104271 cites W1995419427 @default.
- W2782104271 cites W2004450155 @default.
- W2782104271 cites W2007544926 @default.
- W2782104271 cites W2011345608 @default.
- W2782104271 cites W2012906227 @default.
- W2782104271 cites W2017991488 @default.
- W2782104271 cites W2019054401 @default.
- W2782104271 cites W2021039243 @default.
- W2782104271 cites W2023271753 @default.
- W2782104271 cites W2023424060 @default.
- W2782104271 cites W2036114586 @default.
- W2782104271 cites W2037036756 @default.
- W2782104271 cites W2040518676 @default.
- W2782104271 cites W2040720359 @default.
- W2782104271 cites W2041808385 @default.
- W2782104271 cites W2044500327 @default.
- W2782104271 cites W2054525948 @default.
- W2782104271 cites W2069247446 @default.
- W2782104271 cites W2069421427 @default.
- W2782104271 cites W2069705658 @default.
- W2782104271 cites W2084928359 @default.
- W2782104271 cites W2086759672 @default.
- W2782104271 cites W2087333088 @default.
- W2782104271 cites W2088702507 @default.
- W2782104271 cites W2089209891 @default.
- W2782104271 cites W2090457193 @default.
- W2782104271 cites W2090929626 @default.
- W2782104271 cites W2107770760 @default.
- W2782104271 cites W2113847441 @default.
- W2782104271 cites W2122767064 @default.
- W2782104271 cites W2127751112 @default.
- W2782104271 cites W2134226943 @default.
- W2782104271 cites W2134259738 @default.
- W2782104271 cites W2140126848 @default.
- W2782104271 cites W2141440190 @default.
- W2782104271 cites W2143981217 @default.
- W2782104271 cites W2147696493 @default.
- W2782104271 cites W2149666786 @default.
- W2782104271 cites W2160564267 @default.
- W2782104271 cites W2163803692 @default.
- W2782104271 cites W2325527495 @default.
- W2782104271 cites W2328896864 @default.
- W2782104271 cites W4254085062 @default.
- W2782104271 cites W2049392965 @default.
- W2782104271 doi "https://doi.org/10.1039/c7ra11333a" @default.
- W2782104271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35540905" @default.
- W2782104271 hasPublicationYear "2018" @default.
- W2782104271 type Work @default.
- W2782104271 sameAs 2782104271 @default.
- W2782104271 citedByCount "0" @default.
- W2782104271 crossrefType "journal-article" @default.
- W2782104271 hasAuthorship W2782104271A5002509159 @default.
- W2782104271 hasAuthorship W2782104271A5035705840 @default.
- W2782104271 hasAuthorship W2782104271A5065013390 @default.
- W2782104271 hasAuthorship W2782104271A5084805246 @default.
- W2782104271 hasBestOaLocation W27821042711 @default.
- W2782104271 hasConcept C104317684 @default.
- W2782104271 hasConcept C145148216 @default.
- W2782104271 hasConcept C178790620 @default.
- W2782104271 hasConcept C181199279 @default.
- W2782104271 hasConcept C185592680 @default.
- W2782104271 hasConcept C18903297 @default.
- W2782104271 hasConcept C2776834422 @default.
- W2782104271 hasConcept C2777289219 @default.
- W2782104271 hasConcept C2777721518 @default.
- W2782104271 hasConcept C30095370 @default.
- W2782104271 hasConcept C512185932 @default.
- W2782104271 hasConcept C547475151 @default.
- W2782104271 hasConcept C55493867 @default.
- W2782104271 hasConcept C71240020 @default.
- W2782104271 hasConcept C86803240 @default.
- W2782104271 hasConceptScore W2782104271C104317684 @default.
- W2782104271 hasConceptScore W2782104271C145148216 @default.
- W2782104271 hasConceptScore W2782104271C178790620 @default.
- W2782104271 hasConceptScore W2782104271C181199279 @default.
- W2782104271 hasConceptScore W2782104271C185592680 @default.
- W2782104271 hasConceptScore W2782104271C18903297 @default.
- W2782104271 hasConceptScore W2782104271C2776834422 @default.
- W2782104271 hasConceptScore W2782104271C2777289219 @default.
- W2782104271 hasConceptScore W2782104271C2777721518 @default.
- W2782104271 hasConceptScore W2782104271C30095370 @default.
- W2782104271 hasConceptScore W2782104271C512185932 @default.
- W2782104271 hasConceptScore W2782104271C547475151 @default.