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• W2782207943 startingPage "13" @default.
• W2782207943 abstract "Recent studies have revealed that caryophyllene and its stereoisomers not only exhibit multiple biological activities but also have desired properties as renewable candidates for ground transportation and jet fuel applications. This study presents the first significant production of caryophyllene and caryolan-1-ol by an engineered E. coli with heterologous expression of mevalonate pathway genes with a caryophyllene synthase and a caryolan-1-ol synthase. By optimizing metabolic flux and fermentation parameters, the engineered strains yielded 449 mg/L of total terpene, including 406 mg/L sesquiterpene with 100 mg/L caryophyllene and 10 mg/L caryolan-1-ol. Furthermore, a marine microalgae hydrolysate was used as the sole carbon source for the production of caryophyllene and other terpene compounds. Under the optimal fermentation conditions, 360 mg/L of total terpene, 322 mg/L of sesquiterpene, and 75 mg/L caryophyllene were obtained from the pretreated algae hydrolysates. The highest yields achieved on the biomass basis were 48 mg total terpene/g algae and 10 mg caryophyllene/g algae and the caryophyllene yield is approximately ten times higher than that from plant tissues by solvent extraction. The study provides a sustainable alternative for production of caryophyllene and its alcohol from microalgae biomass as potential candidates for next generation aviation fuels." @default.
• W2782207943 created "2018-01-12" @default.
• W2782207943 creator A5026127389 @default.
• W2782207943 creator A5071035208 @default.
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• W2782207943 date "2018-06-01" @default.
• W2782207943 modified "2023-10-13" @default.
• W2782207943 title "Engineering Escherichia coli for the production of terpene mixture enriched in caryophyllene and caryophyllene alcohol as potential aviation fuel compounds" @default.
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• W2782207943 doi "https://doi.org/10.1016/j.meteno.2018.01.001" @default.
• W2782207943 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5767561" @default.
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• W2782207943 hasPublicationYear "2018" @default.
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