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• W2783016456 abstract "Circular permutation is a powerful tool to test the role of topology in protein folding and function. Previous methods for generating circular permutants were based on rearranging gene elements using restriction enzymes-based cloning. Here, we present a Restriction Free (RF) approach to achieve circular permutation which is faster and more cost-effective." @default.
• W2783016456 created "2018-01-26" @default.
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• W2783016456 date "2017-11-29" @default.
• W2783016456 modified "2023-09-25" @default.
• W2783016456 title "Facilitating circular permutation using Restriction Free (RF) cloning" @default.
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• W2783016456 doi "https://doi.org/10.1093/protein/gzx061" @default.
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