FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783106305> ?p ?o ?g. }

• W2783106305 endingPage "164" @default.
• W2783106305 startingPage "160" @default.
• W2783106305 abstract "Monoclonal antibodies (mAbs) have emerged as the most promising category of recombinant proteins due to their high efficiency for the treatment of a wide range of human diseases. The complex nature of mAbs creates a great deal of challenges in both upstream and downstream manufacturing processes. Proportional expression and correct folding and assembly of the light chain and heavy chain are required for efficient production of the mAbs. In this regard, expression vector design has proven to have profound effects on the antibody expression level as well as its stability and quality. Here, we have explored the efficiency of different vector design strategies for the expression of a recombinant IgG1 antibody in Chinese hamster ovary (CHO) cells. The antibody expression level was analyzed in transient expression and stable cell pools followed by expression analysis on single-cell clones. While detectable amounts of antibody were observed in all three systems, dual-promoter single-vector system showed the highest expression level in transient and stable expression as well as the highest productivity among clonal cells. Our results here show the importance of vector design for successful production of whole mAbs in CHO cells." @default.
• W2783106305 created "2018-01-26" @default.
• W2783106305 creator A5000713253 @default.
• W2783106305 creator A5027017805 @default.
• W2783106305 creator A5058303507 @default.
• W2783106305 creator A5089827462 @default.
• W2783106305 creator A5090875607 @default.
• W2783106305 date "2018-02-07" @default.
• W2783106305 modified "2023-09-23" @default.
• W2783106305 title "Evaluation of different vector design strategies for the expression of recombinant monoclonal antibody in CHO cells" @default.
• W2783106305 cites W1199548880 @default.
• W2783106305 cites W1512894213 @default.
• W2783106305 cites W1606589495 @default.
• W2783106305 cites W1966974609 @default.
• W2783106305 cites W1967142377 @default.
• W2783106305 cites W1993617984 @default.
• W2783106305 cites W1995562003 @default.
• W2783106305 cites W2005506831 @default.
• W2783106305 cites W2012688007 @default.
• W2783106305 cites W2013130016 @default.
• W2783106305 cites W2036460884 @default.
• W2783106305 cites W2049915723 @default.
• W2783106305 cites W2071919990 @default.
• W2783106305 cites W2076595618 @default.
• W2783106305 cites W2094052190 @default.
• W2783106305 cites W2146841456 @default.
• W2783106305 cites W2161943011 @default.
• W2783106305 cites W2182396842 @default.
• W2783106305 cites W2257133843 @default.
• W2783106305 cites W2289965054 @default.
• W2783106305 cites W2592640253 @default.
• W2783106305 cites W2608656888 @default.
• W2783106305 doi "https://doi.org/10.1080/10826068.2017.1421966" @default.
• W2783106305 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29313429" @default.
• W2783106305 hasPublicationYear "2018" @default.
• W2783106305 type Work @default.
• W2783106305 sameAs 2783106305 @default.
• W2783106305 citedByCount "22" @default.
• W2783106305 countsByYear W27831063052018 @default.
• W2783106305 countsByYear W27831063052019 @default.
• W2783106305 countsByYear W27831063052020 @default.
• W2783106305 countsByYear W27831063052021 @default.
• W2783106305 countsByYear W27831063052022 @default.
• W2783106305 countsByYear W27831063052023 @default.
• W2783106305 crossrefType "journal-article" @default.
• W2783106305 hasAuthorship W2783106305A5000713253 @default.
• W2783106305 hasAuthorship W2783106305A5027017805 @default.
• W2783106305 hasAuthorship W2783106305A5058303507 @default.
• W2783106305 hasAuthorship W2783106305A5089827462 @default.
• W2783106305 hasAuthorship W2783106305A5090875607 @default.
• W2783106305 hasConcept C100012643 @default.
• W2783106305 hasConcept C104317684 @default.
• W2783106305 hasConcept C153911025 @default.
• W2783106305 hasConcept C159654299 @default.
• W2783106305 hasConcept C175656101 @default.
• W2783106305 hasConcept C203014093 @default.
• W2783106305 hasConcept C33161422 @default.
• W2783106305 hasConcept C36394416 @default.
• W2783106305 hasConcept C40767141 @default.
• W2783106305 hasConcept C542903549 @default.
• W2783106305 hasConcept C54355233 @default.
• W2783106305 hasConcept C81885089 @default.
• W2783106305 hasConcept C86803240 @default.
• W2783106305 hasConcept C92087593 @default.
• W2783106305 hasConcept C95444343 @default.
• W2783106305 hasConceptScore W2783106305C100012643 @default.
• W2783106305 hasConceptScore W2783106305C104317684 @default.
• W2783106305 hasConceptScore W2783106305C153911025 @default.
• W2783106305 hasConceptScore W2783106305C159654299 @default.
• W2783106305 hasConceptScore W2783106305C175656101 @default.
• W2783106305 hasConceptScore W2783106305C203014093 @default.
• W2783106305 hasConceptScore W2783106305C33161422 @default.
• W2783106305 hasConceptScore W2783106305C36394416 @default.
• W2783106305 hasConceptScore W2783106305C40767141 @default.
• W2783106305 hasConceptScore W2783106305C542903549 @default.
• W2783106305 hasConceptScore W2783106305C54355233 @default.
• W2783106305 hasConceptScore W2783106305C81885089 @default.
• W2783106305 hasConceptScore W2783106305C86803240 @default.
• W2783106305 hasConceptScore W2783106305C92087593 @default.
• W2783106305 hasConceptScore W2783106305C95444343 @default.
• W2783106305 hasIssue "2" @default.
• W2783106305 hasLocation W27831063051 @default.
• W2783106305 hasLocation W27831063052 @default.
• W2783106305 hasOpenAccess W2783106305 @default.
• W2783106305 hasPrimaryLocation W27831063051 @default.
• W2783106305 hasRelatedWork W2022691331 @default.
• W2783106305 hasRelatedWork W2049969923 @default.
• W2783106305 hasRelatedWork W2062588824 @default.
• W2783106305 hasRelatedWork W2347915386 @default.
• W2783106305 hasRelatedWork W2351246375 @default.
• W2783106305 hasRelatedWork W2376295338 @default.
• W2783106305 hasRelatedWork W2417074343 @default.
• W2783106305 hasRelatedWork W2497134180 @default.
• W2783106305 hasRelatedWork W2783106305 @default.
• W2783106305 hasRelatedWork W3023702068 @default.
• W2783106305 hasVolume "48" @default.
• W2783106305 isParatext "false" @default.
• W2783106305 isRetracted "false" @default.

• next