FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783109940> ?p ?o ?g. }

• W2783109940 abstract "Vision loss impacts >30 million people worldwide. Mutations in over 50 genes can predispose to the retinal-degenerative disease retinitis pigmentosa (RP). Despite this clear genetic component, disease progression in RP is potentially linked to environmental factors such as light, which can induce the formation of reactive oxygen species leading to oxidative stress and oxidative DNA damage and inducing photoreceptor cell death. Alkylating agents are used to produce animal models of RP as DNA alkylating agents induce photoreceptor cell death. This cytotoxicity is mediated via the DNA repair protein alkyladenine DNA glycosylase (Aag). Hence, we hypothesise that Aag-mediated processing of DNA base damage in photoreceptors affects their function and the light responses in the animal. In-vivo, light/dark cycle entrained wild-type and Aag null mice were treated with methyl methanesulfonate (MMS), under constant light or dark conditions, and harvested 6, 48, and 72-hours post-treatment for histopathological and gene expression analysis. In-vitro, wild-type and Aag null primary embryonic fibroblast cells (pMEFs) were treated with MMS at different circadian phases, and assessed for their circadian phase-response to MMS. Marginal light exacerbation of Aag-mediated retinal-degeneration upon alkylation-exposure is observed in wild-type retinas. Photoreceptor cell function is apparentlyaffected basally and upon alkylation-exposure in wild-type and Aag null mice. Mice melanopsin expressing retinal ganglion cells are resistant to alkylation-damage with no reduction in melanopsin expression. Early gene expression analysis of wild-type and Aag null mice tissues in response to MMS-treatment showed a link between Aag status and upregulation of key cellular stress response mechanisms. MMS seemed to reset the circadian clock in pMEFs in a circadian-time dependent-manner without involving Aag. Aag-deficience can rescue alkylation sensitivity in specific cell types via reducing BER-intermediates accumulation, but Aag is required to play a role in the transcriptional activation of several key genes associated with cell survival, death and function upon alkylation-exposure." @default.
• W2783109940 created "2018-01-26" @default.
• W2783109940 creator A5013652802 @default.
• W2783109940 date "2017-10-31" @default.
• W2783109940 modified "2023-09-22" @default.
• W2783109940 title "Early and late responses to timed alkylation damage in alkyladenine DNA glycosylase animal models." @default.
• W2783109940 cites W138005989 @default.
• W2783109940 cites W1491876634 @default.
• W2783109940 cites W1493170168 @default.
• W2783109940 cites W1495268836 @default.
• W2783109940 cites W1503104403 @default.
• W2783109940 cites W1511847553 @default.
• W2783109940 cites W1549410963 @default.
• W2783109940 cites W1569552610 @default.
• W2783109940 cites W1573169726 @default.
• W2783109940 cites W1587435244 @default.
• W2783109940 cites W1602155299 @default.
• W2783109940 cites W1662741476 @default.
• W2783109940 cites W1695648810 @default.
• W2783109940 cites W1791564905 @default.
• W2783109940 cites W180112362 @default.
• W2783109940 cites W1851766745 @default.
• W2783109940 cites W1859581800 @default.
• W2783109940 cites W1865940872 @default.
• W2783109940 cites W1868062941 @default.
• W2783109940 cites W1893444436 @default.
• W2783109940 cites W1906993142 @default.
• W2783109940 cites W1908546530 @default.
• W2783109940 cites W1935060011 @default.
• W2783109940 cites W1963712961 @default.
• W2783109940 cites W1964740437 @default.
• W2783109940 cites W1965743301 @default.
• W2783109940 cites W1966182868 @default.
• W2783109940 cites W1966655196 @default.
• W2783109940 cites W1967473768 @default.
• W2783109940 cites W1967813793 @default.
• W2783109940 cites W1971524741 @default.
• W2783109940 cites W1971577441 @default.
• W2783109940 cites W1971602039 @default.
• W2783109940 cites W1972021276 @default.
• W2783109940 cites W1972134841 @default.
• W2783109940 cites W1973099196 @default.
• W2783109940 cites W1974527699 @default.
• W2783109940 cites W1976540475 @default.
• W2783109940 cites W1977316202 @default.
• W2783109940 cites W1982138913 @default.
• W2783109940 cites W1987783476 @default.
• W2783109940 cites W1988248815 @default.
• W2783109940 cites W1990264305 @default.
• W2783109940 cites W1991718021 @default.
• W2783109940 cites W1993630555 @default.
• W2783109940 cites W1994120696 @default.
• W2783109940 cites W1994843656 @default.
• W2783109940 cites W1995207708 @default.
• W2783109940 cites W2001902450 @default.
• W2783109940 cites W2003415463 @default.
• W2783109940 cites W2003924215 @default.
• W2783109940 cites W2006946054 @default.
• W2783109940 cites W2007954304 @default.
• W2783109940 cites W2009077913 @default.
• W2783109940 cites W2010029742 @default.
• W2783109940 cites W2010451416 @default.
• W2783109940 cites W2011767660 @default.
• W2783109940 cites W2018893320 @default.
• W2783109940 cites W2023326581 @default.
• W2783109940 cites W2023356259 @default.
• W2783109940 cites W2024486785 @default.
• W2783109940 cites W2025397614 @default.
• W2783109940 cites W2029127611 @default.
• W2783109940 cites W2032463467 @default.
• W2783109940 cites W2035853180 @default.
• W2783109940 cites W2036458630 @default.
• W2783109940 cites W2036903951 @default.
• W2783109940 cites W2038289966 @default.
• W2783109940 cites W2050672796 @default.
• W2783109940 cites W2052978788 @default.
• W2783109940 cites W2058699484 @default.
• W2783109940 cites W2060321052 @default.
• W2783109940 cites W2061217789 @default.
• W2783109940 cites W2061245152 @default.
• W2783109940 cites W2064261105 @default.
• W2783109940 cites W2065990069 @default.
• W2783109940 cites W2067202485 @default.
• W2783109940 cites W2067581325 @default.
• W2783109940 cites W2072063319 @default.
• W2783109940 cites W2072695826 @default.
• W2783109940 cites W2075244890 @default.
• W2783109940 cites W2075609974 @default.
• W2783109940 cites W2075873641 @default.
• W2783109940 cites W2076922446 @default.
• W2783109940 cites W2085459880 @default.
• W2783109940 cites W2087290238 @default.
• W2783109940 cites W2089215158 @default.
• W2783109940 cites W2106249179 @default.
• W2783109940 cites W2106552522 @default.
• W2783109940 cites W2112985897 @default.
• W2783109940 cites W2115104922 @default.
• W2783109940 cites W2119042941 @default.
• W2783109940 cites W2120226804 @default.
• W2783109940 cites W2121075147 @default.

• next