Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783124382> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2783124382 abstract "The use of detergents is often unavoidable in the structural studies of membrane proteins. Dodecylphosphocholine (DPC) is one of the most commonly used detergents for such studies in solution state NMR spectroscopy. The effect of detergent on structure and dynamics remains an important and poorly understood question. In this study we have investigated millisecond dynamics, substrate binding and structural features of three different yeast proteins from mitochondrial carrier family (GGC1, ORC1 and AAC3) in DPC micelles. We have detected millisecond dynamics, which are asymmetrically distributed across the structure. Contrary to previous claims, we show that these dynamics are unrelated to function, as they are not affected by the substitutions which abolish mitochondrial carrier transport in proteoliposomes. Furthermore, we could show that the very well-defined substrate specificity of these proteins in membranes is abolished when they are reconstituted in DPC, questioning their functionality. Structural investigations have revealed that both tertiary and secondary structures of these carriers are perturbed in DPC micelles, with some TM helices showing substantial solvent exposure. We have concluded from these observations that DPC detergent strongly perturbs these, and likely other mitochondrial carriers by rendering them very flexible. Our findings point to a possibly general effect of this detergent on membrane proteins, as we discuss with examples of previously studied membrane proteins. In the second part we have addressed a fundamental question of protein dynamics: how do proteins move inside crystals? We have investigated ms dynamics in a crystalline ubiquitin to gain the insight on the impact of the crystalline lattice on such motions, using solid-state NMR and ms long MD simulations of explicit crystal arrangements. Interestingly a local dynamic exchange process on a ms time scale is still present in crystals. However, by comparing different crystal forms we establish that the thermodynamics of the exchanging states and their interconversion rate constants are significantly altered by the crystal contacts. Furthermore, we detect overall rocking motion of molecules in the crystal, occurring on a tens-of-ms time scale, and provide evidence that overall and local motion are coupled. We discuss the implications of ms dynamics on the data quality in X-ray diffraction experiments." @default.
- W2783124382 created "2018-01-26" @default.
- W2783124382 creator A5000415732 @default.
- W2783124382 date "2017-01-18" @default.
- W2783124382 modified "2023-09-27" @default.
- W2783124382 title "The function of a membrane protein : studies of structure and dynamics by NMR" @default.
- W2783124382 hasPublicationYear "2017" @default.
- W2783124382 type Work @default.
- W2783124382 sameAs 2783124382 @default.
- W2783124382 citedByCount "0" @default.
- W2783124382 crossrefType "dissertation" @default.
- W2783124382 hasAuthorship W2783124382A5000415732 @default.
- W2783124382 hasConcept C11268172 @default.
- W2783124382 hasConcept C12554922 @default.
- W2783124382 hasConcept C144647389 @default.
- W2783124382 hasConcept C147597530 @default.
- W2783124382 hasConcept C147789679 @default.
- W2783124382 hasConcept C159467904 @default.
- W2783124382 hasConcept C184651966 @default.
- W2783124382 hasConcept C185592680 @default.
- W2783124382 hasConcept C191120209 @default.
- W2783124382 hasConcept C2775981520 @default.
- W2783124382 hasConcept C41625074 @default.
- W2783124382 hasConcept C55493867 @default.
- W2783124382 hasConcept C59593255 @default.
- W2783124382 hasConcept C66974803 @default.
- W2783124382 hasConcept C71240020 @default.
- W2783124382 hasConcept C8010536 @default.
- W2783124382 hasConcept C86803240 @default.
- W2783124382 hasConceptScore W2783124382C11268172 @default.
- W2783124382 hasConceptScore W2783124382C12554922 @default.
- W2783124382 hasConceptScore W2783124382C144647389 @default.
- W2783124382 hasConceptScore W2783124382C147597530 @default.
- W2783124382 hasConceptScore W2783124382C147789679 @default.
- W2783124382 hasConceptScore W2783124382C159467904 @default.
- W2783124382 hasConceptScore W2783124382C184651966 @default.
- W2783124382 hasConceptScore W2783124382C185592680 @default.
- W2783124382 hasConceptScore W2783124382C191120209 @default.
- W2783124382 hasConceptScore W2783124382C2775981520 @default.
- W2783124382 hasConceptScore W2783124382C41625074 @default.
- W2783124382 hasConceptScore W2783124382C55493867 @default.
- W2783124382 hasConceptScore W2783124382C59593255 @default.
- W2783124382 hasConceptScore W2783124382C66974803 @default.
- W2783124382 hasConceptScore W2783124382C71240020 @default.
- W2783124382 hasConceptScore W2783124382C8010536 @default.
- W2783124382 hasConceptScore W2783124382C86803240 @default.
- W2783124382 hasLocation W27831243821 @default.
- W2783124382 hasOpenAccess W2783124382 @default.
- W2783124382 hasPrimaryLocation W27831243821 @default.
- W2783124382 hasRelatedWork W125858394 @default.
- W2783124382 hasRelatedWork W1605208918 @default.
- W2783124382 hasRelatedWork W1968299564 @default.
- W2783124382 hasRelatedWork W1968509046 @default.
- W2783124382 hasRelatedWork W1977717875 @default.
- W2783124382 hasRelatedWork W1997150876 @default.
- W2783124382 hasRelatedWork W2007049787 @default.
- W2783124382 hasRelatedWork W2033324060 @default.
- W2783124382 hasRelatedWork W2046854992 @default.
- W2783124382 hasRelatedWork W2050027086 @default.
- W2783124382 hasRelatedWork W2054245178 @default.
- W2783124382 hasRelatedWork W2082926712 @default.
- W2783124382 hasRelatedWork W2097244786 @default.
- W2783124382 hasRelatedWork W2162414164 @default.
- W2783124382 hasRelatedWork W2314956016 @default.
- W2783124382 hasRelatedWork W2327130409 @default.
- W2783124382 hasRelatedWork W2395202574 @default.
- W2783124382 hasRelatedWork W2807137729 @default.
- W2783124382 hasRelatedWork W2981959997 @default.
- W2783124382 hasRelatedWork W322012438 @default.
- W2783124382 isParatext "false" @default.
- W2783124382 isRetracted "false" @default.
- W2783124382 magId "2783124382" @default.
- W2783124382 workType "dissertation" @default.