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- W2783148619 abstract "Abstract A solubilizing Trt‐K 10 tag was developed for the effective chemical preparation of peptides/proteins with low solubility. The Trt‐K 10 tag comprises a hydrophilic oligo‐Lys sequence and a trityl anchor, and can be selectively introduced to a side chain thiol of Cys of deprotected peptides/proteins with a trityl alcohol‐type introducing reagent Trt(OH)‐K 10 under acidic conditions. Significantly, the ligation product in the reaction mixture of a thiol‐additive‐free native chemical ligation can be modified directly in a one‐pot manner to facilitate the isolation of the product by high‐performance liquid chromatography. Finally, the Trt‐K 10 tag can be readily removed with a standard trifluoroacetic acid cocktail. Using this easy‐to‐attach/detach tag‐aided method, a hepatitis B virus capsid protein that is usually difficult to handle was synthesized successfully." @default.
- W2783148619 created "2018-01-26" @default.
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- W2783148619 date "2018-01-25" @default.
- W2783148619 modified "2023-10-09" @default.
- W2783148619 title "Easy-to-Attach/Detach Solubilizing-Tag-Aided Chemical Synthesis of an Aggregative Capsid Protein" @default.
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- W2783148619 doi "https://doi.org/10.1002/anie.201711546" @default.
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- W2783148619 hasPublicationYear "2018" @default.
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