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- W2783165292 abstract "Production costs of F1 seeds of Petunia hybrida can be decreased by using sterile component. Cytoplasmic male sterility (CMS), located in mitochondria, is transferred either by back crossing or by protoplast fusion. Asymmetric protoplast fusion represents the most effective way. Protoplast nucleus of one component (donor of CMS) must be destroyed by UV radiation. Protoplast cytoplasm of the second component must by destroyed by metabolic inhibitors. Asymmetrically fused protoplast possesses CMS from donor and rest of the features of fertile component. The aim of this experiment was to determine the amount of UV radiation (in seconds) needed for destruction of protoplast nucleus. Optimal UV radiation, based on results from 3 different Petunia hybrida genotypes is 510 µW/cm2 for a duration of 300 seconds." @default.
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- W2783165292 date "2017-11-01" @default.
- W2783165292 modified "2023-09-27" @default.
- W2783165292 title "Usage of UV irradiation for nucleus destruction of Petunia hybrida" @default.
- W2783165292 hasPublicationYear "2017" @default.
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