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- W2783174544 abstract "High efficiency separation plays a key role in deep-coverage proteomics studies. In this study, we investigate a mixed-mode chromatographic packing material and use “thiol-ene” click chemistry to prepare a novel reversed-phase/weak-cation-exchange mixed-mode chromatographic packing material. First, we used a mixture of chemical compounds with low molecular weights to characterize the packing material and the results showed that it has both reversed-phase and weak-cation-exchange chromatographic retention mechanisms. Next, we used the reversed-phase/weak-cationic-exchange mixed-mode chromatographic stationary phase in the separation of a mixture of six standard peptides with similar hydrophobicities but with different theoretical isoelectric points. The experimental results showed that the packing materials have a mixed-mode retention performance. We also found that the hydrophilic properties of the embedded functional groups play an important role in decreasing the hydrophobicity of the packing material and therefore reduces the loss of some of the peptides due to the strong adsorption of very high hydrophobic peptides. Lastly, we selected an off-line RP/WCX column with a pH gradient elution as the first dimension for pre-separation and employed reversed-phase liquid chromatography (RPLC)-mass spectrometry analysis as the second dimension for the analysis of the protein mixture of 100-µg HepG2 cells. In total, we identified 5924 proteins. These results are similar to those obtained by the RPLC-mass spectrometry analysis commonly used in proteome research, which suggests that this mixed-mode chromatographic packing material can be used in proteomics research." @default.
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- W2783174544 date "2018-01-04" @default.
- W2783174544 modified "2023-09-24" @default.
- W2783174544 title "Novel reversed-phase/weak-cation-exchange mixed-mode chromatographic packing materials applied in mass-spectrometry-based proteomics" @default.
- W2783174544 doi "https://doi.org/10.1360/n052017-00148" @default.
- W2783174544 hasPublicationYear "2018" @default.
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