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• W2783189911 abstract "Several culture and pre-culture factors affecting ill v i m adventitious bud induction on excised embryos of Swiss stone pine (Pinus cenzbrn L.) were investigatcd. Embryos gave rise to adventitious buds within 4 weeks of culture in a cytokinin-containing medium. Among the tested cytokinins, NLBenzyladenine (BA) proved to bc the most effective, especially when 2.5, or 5 pM were added to the von Arnold and Eriksson's (AE) medium. All the tested pre-induction treatments (4, 8, or 12 week seed chilling. and embryo pre-culture in a hormone-free medium. prior culturing them on the bud induction medium) negligibly affected adventitious budding. Whole embryos were the most suitable explants for bud induction, as poor regeneration was achieved when isolated hypocotyls and epicotyls were cultured. A 21-day exposure of the unchilled explants to BA induced 100% regenerating embryos, and more than 16 buds per explant. Less adventitious shoots were obtained with a 7-day treatment, but shoot growth was moderately accelcrated. Bud development was achieved on a hormone-free AE medium (3 weeks), while shoot elongation was optimized on an activated charcoal-containing (0. 1%) medium (4 weeks). 15 % of the elongated shoots produced adventitious roots, after they were pulsed in liquid l -mM IBA for 5 hours, prior to being transferrred to a hormone-free medium." @default.
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• W2783189911 date "1995-06-23" @default.
• W2783189911 modified "2023-09-23" @default.
• W2783189911 title "Factors affecting in vitro adventitious bud induction from excised embryos of Swiss stone pine (Pinus cembra L.)" @default.
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