FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783195348> ?p ?o ?g. }

• W2783195348 endingPage "3236" @default.
• W2783195348 startingPage "3227" @default.
• W2783195348 abstract "The side chain of Q295 of glycerol-3-phosphate dehydrogenase from human liver (hlGPDH) lies in a flexible loop, that folds over the phosphodianion of substrate dihydroxyacetone phosphate (DHAP). Q295 interacts with the side-chain cation from R269, which is ion-paired to the substrate phosphodianion. Kinetic parameters kcat/Km (M–1 s–1) and kcat/KGAKHPi (M–2 s–1) were determined, respectively, for catalysis of the reduction of DHAP and for dianion activation of catalysis of reduction of glycolaldehyde (GA) catalyzed by wild-type, Q295G, Q295S, Q295A, and Q295N mutants of hlGPDH. These mutations result in up to a 150-fold decrease in (kcat/Km)DHAP and up to a 2.7 kcal/mol decrease in the intrinsic phosphodianion binding energy. The data define a linear correlation with slope 1.1, between the intrinsic phosphodianion binding energy and the intrinsic phosphite dianion binding energy for activation of hlGPDH-catalyzed reduction of GA, that demonstrates a role for Q295 in optimizing this dianion binding energy. The R269A mutation of wild-type GPDH results in a 9.1 kcal/mol destabilization of the transition state for reduction of DHAP, but the same R269A mutation of N270A and Q295A mutants result in smaller 5.9 and 4.9 kcal/mol transition-state destabilization. Similarly, the N270A or Q295A mutations of R269A GPDH each result in large falloffs in the efficiency of rescue of the R269A mutant by guanidine cation. We conclude that N270, which interacts for the substrate phosphodianion and Q295, which interacts with the guanidine side chain of R269, function to optimize the apparent transition-state stabilization provided by the cationic side chain of R269." @default.
• W2783195348 created "2018-01-26" @default.
• W2783195348 creator A5006989072 @default.
• W2783195348 creator A5022708392 @default.
• W2783195348 creator A5062838517 @default.
• W2783195348 creator A5066402611 @default.
• W2783195348 date "2018-01-16" @default.
• W2783195348 modified "2023-10-06" @default.
• W2783195348 title "Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer" @default.
• W2783195348 cites W1542496341 @default.
• W2783195348 cites W1967449452 @default.
• W2783195348 cites W1971026909 @default.
• W2783195348 cites W1981039721 @default.
• W2783195348 cites W1983753605 @default.
• W2783195348 cites W1987038760 @default.
• W2783195348 cites W1991984236 @default.
• W2783195348 cites W1992270716 @default.
• W2783195348 cites W1993916927 @default.
• W2783195348 cites W1994376003 @default.
• W2783195348 cites W2003568586 @default.
• W2783195348 cites W2007272508 @default.
• W2783195348 cites W2007345034 @default.
• W2783195348 cites W2012297445 @default.
• W2783195348 cites W2031965983 @default.
• W2783195348 cites W2041372545 @default.
• W2783195348 cites W2053463812 @default.
• W2783195348 cites W2068971591 @default.
• W2783195348 cites W2079049996 @default.
• W2783195348 cites W2080279475 @default.
• W2783195348 cites W2091639850 @default.
• W2783195348 cites W2094905385 @default.
• W2783195348 cites W2098436230 @default.
• W2783195348 cites W2100366399 @default.
• W2783195348 cites W2102388964 @default.
• W2783195348 cites W2107025108 @default.
• W2783195348 cites W2109926670 @default.
• W2783195348 cites W2137450588 @default.
• W2783195348 cites W2142322696 @default.
• W2783195348 cites W2157606167 @default.
• W2783195348 cites W2167922245 @default.
• W2783195348 cites W2278176906 @default.
• W2783195348 cites W2292814526 @default.
• W2783195348 cites W2313854513 @default.
• W2783195348 cites W2317659294 @default.
• W2783195348 cites W2324375984 @default.
• W2783195348 cites W2334668751 @default.
• W2783195348 cites W2533962253 @default.
• W2783195348 cites W2546164811 @default.
• W2783195348 cites W2558232533 @default.
• W2783195348 cites W4244736178 @default.
• W2783195348 cites W89774207 @default.
• W2783195348 doi "https://doi.org/10.1021/acs.biochem.7b01282" @default.
• W2783195348 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6001809" @default.
• W2783195348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29337541" @default.
• W2783195348 hasPublicationYear "2018" @default.
• W2783195348 type Work @default.
• W2783195348 sameAs 2783195348 @default.
• W2783195348 citedByCount "21" @default.
• W2783195348 countsByYear W27831953482018 @default.
• W2783195348 countsByYear W27831953482019 @default.
• W2783195348 countsByYear W27831953482020 @default.
• W2783195348 countsByYear W27831953482021 @default.
• W2783195348 countsByYear W27831953482022 @default.
• W2783195348 countsByYear W27831953482023 @default.
• W2783195348 crossrefType "journal-article" @default.
• W2783195348 hasAuthorship W2783195348A5006989072 @default.
• W2783195348 hasAuthorship W2783195348A5022708392 @default.
• W2783195348 hasAuthorship W2783195348A5062838517 @default.
• W2783195348 hasAuthorship W2783195348A5066402611 @default.
• W2783195348 hasBestOaLocation W27831953481 @default.
• W2783195348 hasConcept C161790260 @default.
• W2783195348 hasConcept C181199279 @default.
• W2783195348 hasConcept C185592680 @default.
• W2783195348 hasConcept C18903297 @default.
• W2783195348 hasConcept C2776317432 @default.
• W2783195348 hasConcept C2777289219 @default.
• W2783195348 hasConcept C2779617269 @default.
• W2783195348 hasConcept C2780866112 @default.
• W2783195348 hasConcept C41183919 @default.
• W2783195348 hasConcept C55493867 @default.
• W2783195348 hasConcept C56856141 @default.
• W2783195348 hasConcept C71240020 @default.
• W2783195348 hasConcept C86803240 @default.
• W2783195348 hasConceptScore W2783195348C161790260 @default.
• W2783195348 hasConceptScore W2783195348C181199279 @default.
• W2783195348 hasConceptScore W2783195348C185592680 @default.
• W2783195348 hasConceptScore W2783195348C18903297 @default.
• W2783195348 hasConceptScore W2783195348C2776317432 @default.
• W2783195348 hasConceptScore W2783195348C2777289219 @default.
• W2783195348 hasConceptScore W2783195348C2779617269 @default.
• W2783195348 hasConceptScore W2783195348C2780866112 @default.
• W2783195348 hasConceptScore W2783195348C41183919 @default.
• W2783195348 hasConceptScore W2783195348C55493867 @default.
• W2783195348 hasConceptScore W2783195348C56856141 @default.
• W2783195348 hasConceptScore W2783195348C71240020 @default.
• W2783195348 hasConceptScore W2783195348C86803240 @default.
• W2783195348 hasFunder F4320337354 @default.
• W2783195348 hasIssue "23" @default.

• next