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- W278319667 abstract "Aim To obtain a fragment of CYP6 gene from deltamethrin-resistant strain of Aedes albopictus Method A pair of degenerate primers according to the homologus amino acid sequences of CYP6A1,CYP6D1 in Musca domestica and CYP6E1 in Culex quinquefasciatus was designed and used to amplify CYP6 gene from genomic DNA of deltamethrin-resistant strain of Ae albopictus by PCR technique A DNA fragment sized as designed was obtained The fragment was recombinated with pUC19,and cloned into JM109 A positive clone was selected to sequence Homologous analysis on the deduced amino acid sequence was executed A dendrogram was constructed by the use of PC/GENE sequence analysis software Results A nucleotide sequence of 248bp was obtained The cloned sequence identity to members of CYP6 is from 34 2% to 44 3%,to CYP4C1,CYP4D1 and CYP4D2 is 22 1%,29 5% and 27 6% respectively The constructed dendrogram showed a similar result to homologous analysis Conclusion The cloned sequence is a gene fragment from one of members of CYP6 family" @default.
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- W278319667 date "2000-01-01" @default.
- W278319667 modified "2023-09-27" @default.
- W278319667 title "Isolation and identification of a fragment of CYP6 gene from deltamethrin-resistant strain genome of Aedes albopictus." @default.
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