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- W2783209277 abstract "Protocol for plantlets regeneration through callus induction using root meristem was developed in present investigation. Different mediums were standardized for each step vis-a-via callus induction, callus proliferation and shoot regeneration. In first stage, B5 medium added with 0.7mg/l 2, 4-D and 1mg/l BAP showed 100% callusing while reduction in both growth regulators up to level of 0.5 mg/l 2,4-D and 0.1mg/l BAP results in significant callus proliferation. In last step, shoot regeneration was found to good and healthier in B5 medium supplemented with 0.1 mg/l 2, 4-D only. After regeneration normal growth in plantlets observed under plain B-5 medium, however no abnormality has been noted in developed population. Present protocol will be efficiently useful for chemical mutagen induction studies through callus, observing somaclonal variations and transgenic studies." @default.
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- W2783209277 date "2018-01-31" @default.
- W2783209277 modified "2023-09-27" @default.
- W2783209277 title "Three step protocol for regeneration of plantlets in Indian garlic varieties using root meristem" @default.
- W2783209277 doi "https://doi.org/10.18805/ijare.a-4874" @default.
- W2783209277 hasPublicationYear "2018" @default.
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