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- W2783217236 endingPage "1944" @default.
- W2783217236 startingPage "1927" @default.
- W2783217236 abstract "When a stop codon is at the 80S ribosomal A site, there are six nucleotides (+4 to +9) downstream that are inferred to be occupying the mRNA channel. We examined the influence of these downstream nucleotides on translation termination success or failure in mammalian cells at the three stop codons. The expected hierarchy in the intrinsic fidelity of the stop codons (UAA>UAG>>UGA) was observed, with highly influential effects on termination readthrough mediated by nucleotides at position +4 and position +8. A more complex influence was observed from the nucleotides at positions +5 and +6. The weakest termination contexts were most affected by increases or decreases in the concentration of the decoding release factor (eRF1), indicating that eRF1 binding to these signals was rate-limiting. When termination efficiency was significantly reduced by cognate suppressor tRNAs, the observed influence of downstream nucleotides was maintained. There was a positive correlation between experimentally measured signal strength and frequency of the signal in eukaryotic genomes, particularly in Saccharomyces cerevisiae and Drosophila melanogaster. We propose that termination efficiency is not only influenced by interrogation of the stop signal directly by the release factor, but also by downstream ribosomal interactions with the mRNA nucleotides in the entry channel." @default.
- W2783217236 created "2018-01-26" @default.
- W2783217236 creator A5001466717 @default.
- W2783217236 creator A5005878754 @default.
- W2783217236 creator A5006965720 @default.
- W2783217236 creator A5049433648 @default.
- W2783217236 date "2018-01-09" @default.
- W2783217236 modified "2023-10-12" @default.
- W2783217236 title "Eukaryotic translational termination efficiency is influenced by the 3′ nucleotides within the ribosomal mRNA channel" @default.
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