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• W2783229337 endingPage "e0191532" @default.
• W2783229337 startingPage "e0191532" @default.
• W2783229337 abstract "The western blotting technique is widely used to analyze protein expression levels and protein molecular weight. The chemiluminescence method is mainly used for detection due to its high sensitivity and ease of manipulation, but it is unsuitable for detailed analyses because it cannot be used to detect multiple proteins simultaneously. Recently, more attention has been paid to the fluorescence detection method because it is more quantitative and is suitable for the detection of multiple proteins simultaneously. However, fluorescence detection can be limited by poor image resolution and low detection sensitivity. Here, we describe a method to detect fluorescence in western blots using fluorescence microscopy to obtain high-resolution images. In this method, filters and fluorescent dyes are optimized to enhance detection sensitivity to a level similar to that of the chemiluminescence method." @default.
• W2783229337 created "2018-01-26" @default.
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• W2783229337 date "2018-01-19" @default.
• W2783229337 modified "2023-10-12" @default.
• W2783229337 title "Sensitive detection of fluorescence in western blotting by merging images" @default.
• W2783229337 cites W185150389 @default.
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• W2783229337 doi "https://doi.org/10.1371/journal.pone.0191532" @default.
• W2783229337 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5774814" @default.
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• W2783229337 hasPublicationYear "2018" @default.
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