FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783232660> ?p ?o ?g. }

• W2783232660 endingPage "501" @default.
• W2783232660 startingPage "485" @default.
• W2783232660 abstract "Mountain pine beetle (Dendroctonus ponderosae Hopkins; MPB) is an economically and ecologically important pest of pine species in western North America. Mountain pine beetles form complex multipartite relationships with microbial partners, including the ophiostomoid fungi Grosmannia clavigera (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield, Ophiostoma montium (Rumbold) von Arx, Grosmannia aurea (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield, Leptographium longiclavatum (Lee, Kim, and Breuil) and Leptographium terebrantis (Barras and Perry). These fungi are vectored by MPB to new pine hosts, where the fungi overcome host defenses to grow into the sapwood. A tree's relative susceptibility to these fungi is conventionally assessed by measuring lesions that develop in response to fungal inoculation. However, these lesions represent a symptom of infection, representing both fungal growth and tree defense capacity. In order to more objectively assess fungal virulence and host tree susceptibility in studies of host-pathogen interactions, a reliable, consistent, sensitive method is required to accurately identify and quantify MPB-associated fungal symbionts in planta. We have adapted RNase H2-dependent PCR, a technique originally designed for rare allele discrimination, to develop a novel RNase H2-dependent quantitative PCR (rh-qPCR) assay that shows greater specificity and sensitivity than previously published PCR-based methods to quantify MPB fungal symbionts in pine xylem and MPB whole beetles. Two sets of assay probes were designed: one that amplifies a broad range of ophiostomoid species, and a second that amplifies G. clavigera but not other MPB-associated ophiostomoid species. Using these primers to quantify G. clavigera in pine stems, we provide evidence that lesion length does not accurately reflect the extent of fungal colonization along the stem nor the quantity of fungal growth within this colonized portion of stem. The sensitivity, specificity, reproducibility, cost effectiveness and high-throughput potential of the rh-qPCR assay makes the technology suitable for identification and quantification of a wide array of pathogenic and beneficial microbes that form associations with plants and other organisms, even when the microbial partner is present in low abundance." @default.
• W2783232660 created "2018-01-26" @default.
• W2783232660 creator A5012544609 @default.
• W2783232660 creator A5016124479 @default.
• W2783232660 creator A5032086005 @default.
• W2783232660 creator A5047100923 @default.
• W2783232660 creator A5062426715 @default.
• W2783232660 creator A5080271282 @default.
• W2783232660 creator A5085988251 @default.
• W2783232660 date "2018-01-10" @default.
• W2783232660 modified "2023-10-18" @default.
• W2783232660 title "A novel application of RNase H2-dependent quantitative PCR for detection and quantification of Grosmannia clavigera, a mountain pine beetle fungal symbiont, in environmental samples" @default.
• W2783232660 cites W1547115433 @default.
• W2783232660 cites W1580475794 @default.
• W2783232660 cites W1763779503 @default.
• W2783232660 cites W1892049120 @default.
• W2783232660 cites W1982496659 @default.
• W2783232660 cites W1989295100 @default.
• W2783232660 cites W1999949720 @default.
• W2783232660 cites W2000114960 @default.
• W2783232660 cites W2012742319 @default.
• W2783232660 cites W2016608544 @default.
• W2783232660 cites W2016918002 @default.
• W2783232660 cites W2017151971 @default.
• W2783232660 cites W2030773179 @default.
• W2783232660 cites W2033609627 @default.
• W2783232660 cites W2033908496 @default.
• W2783232660 cites W2044324217 @default.
• W2783232660 cites W2046747902 @default.
• W2783232660 cites W2049869547 @default.
• W2783232660 cites W2064240240 @default.
• W2783232660 cites W2065213186 @default.
• W2783232660 cites W2067107431 @default.
• W2783232660 cites W2089432176 @default.
• W2783232660 cites W2092686336 @default.
• W2783232660 cites W2095936286 @default.
• W2783232660 cites W2096322745 @default.
• W2783232660 cites W2096673997 @default.
• W2783232660 cites W2098783654 @default.
• W2783232660 cites W2099598840 @default.
• W2783232660 cites W2103673599 @default.
• W2783232660 cites W2107721649 @default.
• W2783232660 cites W2109176572 @default.
• W2783232660 cites W2112324446 @default.
• W2783232660 cites W2115265399 @default.
• W2783232660 cites W2120311182 @default.
• W2783232660 cites W2122139993 @default.
• W2783232660 cites W2130479858 @default.
• W2783232660 cites W2131418695 @default.
• W2783232660 cites W2139136088 @default.
• W2783232660 cites W2139738409 @default.
• W2783232660 cites W2148652112 @default.
• W2783232660 cites W2150358049 @default.
• W2783232660 cites W2150569514 @default.
• W2783232660 cites W2150998923 @default.
• W2783232660 cites W2153768827 @default.
• W2783232660 cites W2155703860 @default.
• W2783232660 cites W2156456843 @default.
• W2783232660 cites W2156831884 @default.
• W2783232660 cites W2160012365 @default.
• W2783232660 cites W2162382248 @default.
• W2783232660 cites W2163541402 @default.
• W2783232660 cites W2166425941 @default.
• W2783232660 cites W2170218835 @default.
• W2783232660 cites W2170915073 @default.
• W2783232660 cites W2174071963 @default.
• W2783232660 cites W2175080427 @default.
• W2783232660 cites W2175435889 @default.
• W2783232660 cites W2200908218 @default.
• W2783232660 cites W2464159242 @default.
• W2783232660 cites W2469214530 @default.
• W2783232660 cites W2502739852 @default.
• W2783232660 cites W2531352136 @default.
• W2783232660 cites W2532899471 @default.
• W2783232660 cites W2589703780 @default.
• W2783232660 cites W2620426489 @default.
• W2783232660 cites W4229580415 @default.
• W2783232660 cites W4247929920 @default.
• W2783232660 doi "https://doi.org/10.1093/treephys/tpx147" @default.
• W2783232660 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5982843" @default.
• W2783232660 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29329457" @default.
• W2783232660 hasPublicationYear "2018" @default.
• W2783232660 type Work @default.
• W2783232660 sameAs 2783232660 @default.
• W2783232660 citedByCount "9" @default.
• W2783232660 countsByYear W27832326602018 @default.
• W2783232660 countsByYear W27832326602019 @default.
• W2783232660 countsByYear W27832326602021 @default.
• W2783232660 countsByYear W27832326602023 @default.
• W2783232660 crossrefType "journal-article" @default.
• W2783232660 hasAuthorship W2783232660A5012544609 @default.
• W2783232660 hasAuthorship W2783232660A5016124479 @default.
• W2783232660 hasAuthorship W2783232660A5032086005 @default.
• W2783232660 hasAuthorship W2783232660A5047100923 @default.
• W2783232660 hasAuthorship W2783232660A5062426715 @default.
• W2783232660 hasAuthorship W2783232660A5080271282 @default.
• W2783232660 hasAuthorship W2783232660A5085988251 @default.
• W2783232660 hasBestOaLocation W27832326601 @default.

• next