Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783247047> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2783247047 endingPage "105" @default.
- W2783247047 startingPage "95" @default.
- W2783247047 abstract "Hypoxic conditions in the pancreatic tumor microenvironment lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which acts as the master regulator of cancer cell metabolism. HIF-1α-mediated metabolic reprogramming results in large-scale metabolite perturbations. Characterization of the metabolic intermediates and the corresponding metabolic pathways altered by HIF-1α would facilitate the identification of therapeutic targets for hypoxic microenvironments prevalent in pancreatic ductal adenocarcinoma and other solid tumors. Targeted metabolomic approaches are versatile in quantifying multiple metabolite levels in a single platform and, thus, enable the characterization of multiple metabolite alterations regulated by HIF-1α. In this chapter, we describe a detailed metabolomic approach for characterizing the hypoxia-induced metabolomic alterations using pancreatic cancer cell lines cultured in normoxic and hypoxic conditions. We elaborate the methodology of cell culture, hypoxic exposure, metabolite extraction, and relative quantification of polar metabolites from normoxia- and hypoxia-exposed cell extracts, using a liquid chromatography-coupled tandem mass spectrometry approach. Herein, using our metabolomic data, we also present the methods for metabolomic data representation." @default.
- W2783247047 created "2018-01-26" @default.
- W2783247047 creator A5001608330 @default.
- W2783247047 creator A5041700915 @default.
- W2783247047 creator A5067292361 @default.
- W2783247047 creator A5086534465 @default.
- W2783247047 date "2018-01-01" @default.
- W2783247047 modified "2023-10-03" @default.
- W2783247047 title "Hypoxia-Induced Metabolomic Alterations in Pancreatic Cancer Cells" @default.
- W2783247047 cites W1484579780 @default.
- W2783247047 cites W1984060145 @default.
- W2783247047 cites W2002215164 @default.
- W2783247047 cites W2033037709 @default.
- W2783247047 cites W2057723060 @default.
- W2783247047 cites W2068936088 @default.
- W2783247047 cites W2108775189 @default.
- W2783247047 cites W2118917780 @default.
- W2783247047 cites W2342789743 @default.
- W2783247047 cites W2735063286 @default.
- W2783247047 cites W2738781337 @default.
- W2783247047 cites W4210738594 @default.
- W2783247047 doi "https://doi.org/10.1007/978-1-4939-7665-2_9" @default.
- W2783247047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29330793" @default.
- W2783247047 hasPublicationYear "2018" @default.
- W2783247047 type Work @default.
- W2783247047 sameAs 2783247047 @default.
- W2783247047 citedByCount "11" @default.
- W2783247047 countsByYear W27832470472019 @default.
- W2783247047 countsByYear W27832470472020 @default.
- W2783247047 countsByYear W27832470472021 @default.
- W2783247047 countsByYear W27832470472022 @default.
- W2783247047 countsByYear W27832470472023 @default.
- W2783247047 crossrefType "book-chapter" @default.
- W2783247047 hasAuthorship W2783247047A5001608330 @default.
- W2783247047 hasAuthorship W2783247047A5041700915 @default.
- W2783247047 hasAuthorship W2783247047A5067292361 @default.
- W2783247047 hasAuthorship W2783247047A5086534465 @default.
- W2783247047 hasConcept C121608353 @default.
- W2783247047 hasConcept C178790620 @default.
- W2783247047 hasConcept C185592680 @default.
- W2783247047 hasConcept C192989942 @default.
- W2783247047 hasConcept C21565614 @default.
- W2783247047 hasConcept C2777477808 @default.
- W2783247047 hasConcept C2780210213 @default.
- W2783247047 hasConcept C540031477 @default.
- W2783247047 hasConcept C54355233 @default.
- W2783247047 hasConcept C55493867 @default.
- W2783247047 hasConcept C60644358 @default.
- W2783247047 hasConcept C62231903 @default.
- W2783247047 hasConcept C7836513 @default.
- W2783247047 hasConcept C86803240 @default.
- W2783247047 hasConceptScore W2783247047C121608353 @default.
- W2783247047 hasConceptScore W2783247047C178790620 @default.
- W2783247047 hasConceptScore W2783247047C185592680 @default.
- W2783247047 hasConceptScore W2783247047C192989942 @default.
- W2783247047 hasConceptScore W2783247047C21565614 @default.
- W2783247047 hasConceptScore W2783247047C2777477808 @default.
- W2783247047 hasConceptScore W2783247047C2780210213 @default.
- W2783247047 hasConceptScore W2783247047C540031477 @default.
- W2783247047 hasConceptScore W2783247047C54355233 @default.
- W2783247047 hasConceptScore W2783247047C55493867 @default.
- W2783247047 hasConceptScore W2783247047C60644358 @default.
- W2783247047 hasConceptScore W2783247047C62231903 @default.
- W2783247047 hasConceptScore W2783247047C7836513 @default.
- W2783247047 hasConceptScore W2783247047C86803240 @default.
- W2783247047 hasLocation W27832470471 @default.
- W2783247047 hasLocation W27832470472 @default.
- W2783247047 hasOpenAccess W2783247047 @default.
- W2783247047 hasPrimaryLocation W27832470471 @default.
- W2783247047 hasRelatedWork W2020922083 @default.
- W2783247047 hasRelatedWork W2119215916 @default.
- W2783247047 hasRelatedWork W2156185846 @default.
- W2783247047 hasRelatedWork W2912122986 @default.
- W2783247047 hasRelatedWork W2921361779 @default.
- W2783247047 hasRelatedWork W2947387731 @default.
- W2783247047 hasRelatedWork W2948400285 @default.
- W2783247047 hasRelatedWork W3009472108 @default.
- W2783247047 hasRelatedWork W3169423050 @default.
- W2783247047 hasRelatedWork W3209673460 @default.
- W2783247047 isParatext "false" @default.
- W2783247047 isRetracted "false" @default.
- W2783247047 magId "2783247047" @default.
- W2783247047 workType "book-chapter" @default.