FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783279718> ?p ?o ?g. }

• W2783279718 endingPage "98" @default.
• W2783279718 startingPage "84" @default.
• W2783279718 abstract "Background: Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients.Methods: Antigen “capture” antibodies for midkine, syndecan-1, and ANGPTL4 were independently conjugated to MagPlex® Microspheres using standard carbodiimide/NHS-based chemistry. These reagents served as the basis for quantitative sandwich assay assembly using biotinylated detection antibodies and R-phycoerythrin-conjugated streptavidin reporter system. Standard curves were created using dilution series of recombinant target proteins with assay performance characteristics calculated, accordingly. Finally, we evaluated a range of serum samples from NSCLC patients (n = 32) to verify assay performance.Results: Multiplexed assays for midkine, syndecan-1, and ANGPTL4 were developed with three orders of magnitude in dynamic range, excellent intra- and inter-assay precision, and accuracy parameters (<10%, and <15% variability, respectively). Detection and quantifications limits were suitable for the three assays to efficiently evaluate sera across a range of disease stages with a four-fold dilution factor.Conclusion: We successfully developed and analytically validated a 3-plex immunobead assay for quantifying midkine, syndecan-1, and ANGPTL4 in patient sera. This multiplexed assay will provide an important tool for future studies delineating the role of angiogenesis in lung cancer progression." @default.
• W2783279718 created "2018-01-26" @default.
• W2783279718 creator A5024343309 @default.
• W2783279718 creator A5029075855 @default.
• W2783279718 creator A5032871452 @default.
• W2783279718 creator A5053832363 @default.
• W2783279718 creator A5076167727 @default.
• W2783279718 creator A5078163512 @default.
• W2783279718 creator A5081097197 @default.
• W2783279718 date "2018-01-02" @default.
• W2783279718 modified "2023-09-29" @default.
• W2783279718 title "Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serum" @default.
• W2783279718 cites W1499046649 @default.
• W2783279718 cites W1544857309 @default.
• W2783279718 cites W1985037211 @default.
• W2783279718 cites W1990791138 @default.
• W2783279718 cites W2001010891 @default.
• W2783279718 cites W2021642920 @default.
• W2783279718 cites W2045800415 @default.
• W2783279718 cites W2097381469 @default.
• W2783279718 cites W2099582683 @default.
• W2783279718 cites W2116490593 @default.
• W2783279718 cites W2133094954 @default.
• W2783279718 cites W2194457498 @default.
• W2783279718 cites W2331527284 @default.
• W2783279718 cites W2377002235 @default.
• W2783279718 cites W2539613600 @default.
• W2783279718 cites W4234412286 @default.
• W2783279718 cites W62697226 @default.
• W2783279718 cites W806224767 @default.
• W2783279718 doi "https://doi.org/10.1080/15321819.2017.1407338" @default.
• W2783279718 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8328281" @default.
• W2783279718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29309212" @default.
• W2783279718 hasPublicationYear "2018" @default.
• W2783279718 type Work @default.
• W2783279718 sameAs 2783279718 @default.
• W2783279718 citedByCount "8" @default.
• W2783279718 countsByYear W27832797182018 @default.
• W2783279718 countsByYear W27832797182021 @default.
• W2783279718 countsByYear W27832797182022 @default.
• W2783279718 countsByYear W27832797182023 @default.
• W2783279718 crossrefType "journal-article" @default.
• W2783279718 hasAuthorship W2783279718A5024343309 @default.
• W2783279718 hasAuthorship W2783279718A5029075855 @default.
• W2783279718 hasAuthorship W2783279718A5032871452 @default.
• W2783279718 hasAuthorship W2783279718A5053832363 @default.
• W2783279718 hasAuthorship W2783279718A5076167727 @default.
• W2783279718 hasAuthorship W2783279718A5078163512 @default.
• W2783279718 hasAuthorship W2783279718A5081097197 @default.
• W2783279718 hasBestOaLocation W27832797182 @default.
• W2783279718 hasConcept C128972844 @default.
• W2783279718 hasConcept C143998085 @default.
• W2783279718 hasConcept C153911025 @default.
• W2783279718 hasConcept C158074577 @default.
• W2783279718 hasConcept C159654299 @default.
• W2783279718 hasConcept C170493617 @default.
• W2783279718 hasConcept C185592680 @default.
• W2783279718 hasConcept C203014093 @default.
• W2783279718 hasConcept C204628709 @default.
• W2783279718 hasConcept C2775960820 @default.
• W2783279718 hasConcept C2776190903 @default.
• W2783279718 hasConcept C2776256026 @default.
• W2783279718 hasConcept C2778204606 @default.
• W2783279718 hasConcept C2780394083 @default.
• W2783279718 hasConcept C2780420688 @default.
• W2783279718 hasConcept C502942594 @default.
• W2783279718 hasConcept C55493867 @default.
• W2783279718 hasConcept C71924100 @default.
• W2783279718 hasConcept C86803240 @default.
• W2783279718 hasConceptScore W2783279718C128972844 @default.
• W2783279718 hasConceptScore W2783279718C143998085 @default.
• W2783279718 hasConceptScore W2783279718C153911025 @default.
• W2783279718 hasConceptScore W2783279718C158074577 @default.
• W2783279718 hasConceptScore W2783279718C159654299 @default.
• W2783279718 hasConceptScore W2783279718C170493617 @default.
• W2783279718 hasConceptScore W2783279718C185592680 @default.
• W2783279718 hasConceptScore W2783279718C203014093 @default.
• W2783279718 hasConceptScore W2783279718C204628709 @default.
• W2783279718 hasConceptScore W2783279718C2775960820 @default.
• W2783279718 hasConceptScore W2783279718C2776190903 @default.
• W2783279718 hasConceptScore W2783279718C2776256026 @default.
• W2783279718 hasConceptScore W2783279718C2778204606 @default.
• W2783279718 hasConceptScore W2783279718C2780394083 @default.
• W2783279718 hasConceptScore W2783279718C2780420688 @default.
• W2783279718 hasConceptScore W2783279718C502942594 @default.
• W2783279718 hasConceptScore W2783279718C55493867 @default.
• W2783279718 hasConceptScore W2783279718C71924100 @default.
• W2783279718 hasConceptScore W2783279718C86803240 @default.
• W2783279718 hasIssue "1" @default.
• W2783279718 hasLocation W27832797181 @default.
• W2783279718 hasLocation W27832797182 @default.
• W2783279718 hasOpenAccess W2783279718 @default.
• W2783279718 hasPrimaryLocation W27832797181 @default.
• W2783279718 hasRelatedWork W1989621679 @default.
• W2783279718 hasRelatedWork W2082471181 @default.
• W2783279718 hasRelatedWork W2103049653 @default.
• W2783279718 hasRelatedWork W2113451618 @default.
• W2783279718 hasRelatedWork W2136004642 @default.

• next