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• W2783289525 abstract "Background: The squamous cell carcinoma of the tongue (TSCC) is the mostfrequent cancer of oral cavity and is extremely aggressive and characterized by poorprognosis. It is a complex disease to be treated and therapies in use have led tomediocre results and many side effects. Some facts suggest that natural essences cansupport traditional cancer therapy, carrying out a synergistic function withchemotherapyIn particular, curcumin is used for decades in Chinese medicine for its beneficialeffects, while genistein is an isoflavone that is known as an angiogenesis inhibitorand a phytoestrogenCurcumin and genistein have anticancer properties in many tumors but their actionon the tongue carcinoma is not entirely clear and many other investigations arenecessary.Methods: In this study, we evaluated the effects of curcumin on TSCC cells usingdifferent concentrations (1, 5, 10, 20 and 50 μM) and 3 different treatment times (24,48 and 72 hours). Furthermore, 20, 50 and 100 μM of genistein are used at the sametime points. The inhibition of adhesion, proliferation, viability, migration andtumorigenesis were studied. Calcium Colorimetric Assay Kit, ATP-measurementmethods, LDH Cytotoxicity Assay, quantitative determination of intracellular ROSlevel, analysis of ΔΨm by fluorescence assay were used to determinate biochemicalchanges responsible for cellular injury.Results: We monitored in real time the growth kinetics for 72 hours after treatmentand all CI values analyzed. The adhesion interval was 0-10 hours.We found a halving of cell adhesion after treatment with 5 μM of curcumin, a ~ 25%reduction after treatment with 1 μM of curcumin. Furthermore, adhesion of TSCCcells treated with 50 μM of curcumin was reduced by 75% compared to control.Even, the down-regulation of integrin expression supports the theory goes thatcurcumin inhibits adhesion of the tongue cancer cells.The proliferation is reduced by 50% after treatment with 5-10μM curcumin at all timepoints considered and IC50 value is ~ 10 μM. Curcumin reduces vitality, migrationand progression of TSCC cells and it promotes apoptosis and inhibits tumorigenesis.In fact, PAR 4 is increased after curcumin treatments while Survivin and Oct4decreased.While, cell adhesion of TSCC cells is inhibited especially between 20 and 50 μM ofgenistein treatment. Proliferation is reduced by 50% for treatments with 20 μM at 24hours, with 20 or 50 μM at 48 and 50 μM at 72 hours (p <0.0001). The viability andmigration appeared to be reduced with high significance (p <0.001). Genistein downregulatedvitronectin, oct4 and survivin. It was showed an increase of intracellularCa2+ and LDH release while there was a reduction of ATP levels after treatments ofcurcumin and genistein. Also ROS and ΔΨm were reduced after treatments. Thesebiochemical changes have confirmed the cell damage and consequently apoptosisafter treatments.Conclusions: These results suggest the possible use of curcumin and genistein asanticancer agents in TSCC. In vivo studies are needed to confirm these data and tobe able to manufacture a suitable delivery system that is acting directly in the tumorsite." @default.
• W2783289525 created "2018-01-26" @default.
• W2783289525 creator A5056827709 @default.
• W2783289525 date "2017-01-01" @default.
• W2783289525 modified "2023-09-23" @default.
• W2783289525 title "In vitro study on anticancer properties of Curcumin and Genistein in Squamous Cell Carcinoma of Tongue" @default.
• W2783289525 doi "https://doi.org/10.14274/ardito-fatima_phd2017" @default.
• W2783289525 hasPublicationYear "2017" @default.
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