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• W2783409590 abstract "The low seed yield of Jatropha curcas has been a stumbling block in realizing its full potential as an ideal bioenergy crop. Low female to male flower ratio is considered as a major limiting factor responsible for low seed yield in Jatropha. An exogenous cytokinin application was performed on floral meristems to increase the seed yield. This resulted in an increase of total flowers count with a higher female to male flower ratio. However, the seed biomass did not increase in the same proportion. The possible reason for this was hypothesized to be the lack of increased photosynthesis efficiency at source tissues which could fulfil the increased demand of photosynthates and primary metabolites in maturing seeds. After cytokinin application, possible molecular mechanisms underlying carbon capture and flux affected between the source and sink in developing flowers, fruits and seeds were investigated. Comparative transcriptome analysis was performed on inflorescence meristems (treated with cytokinin) and control (untreated inflorescence meristems) at time intervals of 15 and 30 days, respectively. KEGG-based functional annotation identified various metabolic pathways associated with carbon capture and flux. Pathways such as photosynthesis, carbon fixation, carbohydrate metabolism and nitrogen metabolism were upregulated after 15 days of cytokinin treatment; however, those were downregulated after 30 days. Five genes FBP, SBP, GS, GDH and AGPase showed significant increase in transcript abundance after 15 days of treatment but showed a significant decrease after 30 days. These genes, after functional validation, can be suitable targets in designing a suitable genetic intervention strategy to increase overall seed yield in Jatropha." @default.
• W2783409590 created "2018-01-26" @default.
• W2783409590 creator A5005833533 @default.
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• W2783409590 creator A5080185322 @default.
• W2783409590 date "2018-01-01" @default.
• W2783409590 modified "2023-10-18" @default.
• W2783409590 title "Comparative transcriptomics reveals a reduction in carbon capture and flux between source and sink in cytokinin-treated inflorescences of Jatropha curcas L." @default.
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• W2783409590 doi "https://doi.org/10.1007/s13205-018-1089-2" @default.
• W2783409590 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5764882" @default.
• W2783409590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29354375" @default.
• W2783409590 hasPublicationYear "2018" @default.
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