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• W2783414570 abstract "ABSTRACT Methyl coenzyme M reductase (MCR) is a complex enzyme that catalyzes the final step in biological methanogenesis. To better understand its assembly, the recombinant MCR from the thermophile Methanothermococcus okinawensis (rMCR ok ) was expressed in the mesophile Methanococcus maripaludis . The rMCR ok was posttranslationally modified correctly and contained McrD and the unique nickel tetrapyrrole coenzyme F 430 . Subunits of the native M. maripaludis (MCR mar ) were largely absent, suggesting that the recombinant enzyme was formed by an assembly of cotranscribed subunits. Strong support for this hypothesis was obtained by expressing a chimeric operon comprising the His-tagged mcrA from M. maripaludis and the mcrBDCG from M. okinawensis in M. maripaludis . The His-tagged purified rMCR then contained the M. maripaludis McrA and the M. okinawensis McrBDG. The present study prompted us to form a working model for MCR assembly, which can be further tested by the heterologous expression system established here. IMPORTANCE Approximately 1.6% of the net primary production of plants, algae, and cyanobacteria are processed by biological methane production in anoxic environments. This accounts for about 74% of the total global methane production, up to 25% of which is consumed by anaerobic oxidation of methane (AOM). Methyl coenzyme M reductase (MCR) is the key enzyme in both methanogenesis and AOM. MCR is assembled as a dimer of two heterotrimers, where posttranslational modifications and F 430 cofactors are embedded in the active sites. However, this complex assembly process remains unknown. Here, we established a heterologous expression system for MCR to learn how MCR is assembled." @default.
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• W2783414570 date "2018-04-01" @default.
• W2783414570 modified "2023-10-10" @default.
• W2783414570 title "Assembly of Methyl Coenzyme M Reductase in the Methanogenic Archaeon Methanococcus maripaludis" @default.
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• W2783414570 doi "https://doi.org/10.1128/jb.00746-17" @default.
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