FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783448603> ?p ?o ?g. }

• W2783448603 endingPage "442" @default.
• W2783448603 startingPage "433" @default.
• W2783448603 abstract "ObjectivesArteriovenous malformations (AVMs) are complex vascular lesions. Surgical excision is the treatment of choice, but is often not achievable. Embolo-sclerotherapy alone is associated with high recurrence rates. This study tested the hypothesis that seeding hydrogel conditioned bone marrow derived mesenchymatous stem cells (BM-MSCs) in an AVM nidus model induces solid microvascular occlusion through endoluminal tissue growth.MethodsAVMs were modelled as arteriovenous microvascular nidus, using swine rete mirabile, a plexiform intracranial structure composed of arterial microvessels that extensively anastomose. A right carotid-jugular fistula was created to generate high flow in the rete, and bone marrow was aspirated. At day 14, cultured BM-MSCs marked with a red fluorochrome were incorporated into a hyaluronic acid hydrogel, and injected through a catheter into the rete mirabile, using femoral access. In specific groups microsphere embolisation immediately preceded gel injection. At day 28, the swine were euthanased and the rete mirabile harvested for qualitative and quantitative analysis of microvessel lumen occlusion.ResultsActual transfer of PKH26 labelled cells in rete was confirmed. In a first phase of the study, five swine died as a result of neurological events, prompting reductions of the injected volumes. Twenty-three animals survived until day 28. Injection of BM-MSC loaded hydrogel (n=6) significantly increased the occlusion rate compared with injection of acellular hydrogel (n=7) (10% [range, 10–12%] vs. 26% [range, 20–41%], p=.016). Injection of BM-MSC loaded hydrogel immediately after microspheres (n=6) enhanced the occlusion rate compared with embolic microspheres alone (n=6) (50% [range 46–56%] vs. 22% [range, 15–27%] p=.045). Microsphere injection resulted in vascular luminal thrombus formation. Injection of BM-MSCs induced endoluminal growth of vascular smooth muscle cells with cell proliferation and a dense collagen rich extracellular matrix.ConclusionThe luminal occlusion pattern of a microvascular AVM like structure can be changed from thrombus to solid cellular accumulation. The possibility of injecting living cells in a microvascular network, in a chronic survival model, provides new tools for research and treatment of AVMs and other microvascular diseases. Arteriovenous malformations (AVMs) are complex vascular lesions. Surgical excision is the treatment of choice, but is often not achievable. Embolo-sclerotherapy alone is associated with high recurrence rates. This study tested the hypothesis that seeding hydrogel conditioned bone marrow derived mesenchymatous stem cells (BM-MSCs) in an AVM nidus model induces solid microvascular occlusion through endoluminal tissue growth. AVMs were modelled as arteriovenous microvascular nidus, using swine rete mirabile, a plexiform intracranial structure composed of arterial microvessels that extensively anastomose. A right carotid-jugular fistula was created to generate high flow in the rete, and bone marrow was aspirated. At day 14, cultured BM-MSCs marked with a red fluorochrome were incorporated into a hyaluronic acid hydrogel, and injected through a catheter into the rete mirabile, using femoral access. In specific groups microsphere embolisation immediately preceded gel injection. At day 28, the swine were euthanased and the rete mirabile harvested for qualitative and quantitative analysis of microvessel lumen occlusion. Actual transfer of PKH26 labelled cells in rete was confirmed. In a first phase of the study, five swine died as a result of neurological events, prompting reductions of the injected volumes. Twenty-three animals survived until day 28. Injection of BM-MSC loaded hydrogel (n=6) significantly increased the occlusion rate compared with injection of acellular hydrogel (n=7) (10% [range, 10–12%] vs. 26% [range, 20–41%], p=.016). Injection of BM-MSC loaded hydrogel immediately after microspheres (n=6) enhanced the occlusion rate compared with embolic microspheres alone (n=6) (50% [range 46–56%] vs. 22% [range, 15–27%] p=.045). Microsphere injection resulted in vascular luminal thrombus formation. Injection of BM-MSCs induced endoluminal growth of vascular smooth muscle cells with cell proliferation and a dense collagen rich extracellular matrix. The luminal occlusion pattern of a microvascular AVM like structure can be changed from thrombus to solid cellular accumulation. The possibility of injecting living cells in a microvascular network, in a chronic survival model, provides new tools for research and treatment of AVMs and other microvascular diseases." @default.
• W2783448603 created "2018-01-26" @default.
• W2783448603 creator A5027008477 @default.
• W2783448603 creator A5029041534 @default.
• W2783448603 creator A5044775777 @default.
• W2783448603 creator A5080447163 @default.
• W2783448603 creator A5083472385 @default.
• W2783448603 date "2018-03-01" @default.
• W2783448603 modified "2023-10-18" @default.
• W2783448603 title "Catheter Injected Bone Marrow Mesenchymal Stem Cells Induce Efficacious Occlusion of Arteriovenous Nidus in a Swine Model" @default.
• W2783448603 cites W1970590929 @default.
• W2783448603 cites W1978460113 @default.
• W2783448603 cites W1985659242 @default.
• W2783448603 cites W2037810763 @default.
• W2783448603 cites W2054164536 @default.
• W2783448603 cites W2061528799 @default.
• W2783448603 cites W2078286853 @default.
• W2783448603 cites W2081245133 @default.
• W2783448603 cites W2091573294 @default.
• W2783448603 cites W2092962260 @default.
• W2783448603 cites W2115695634 @default.
• W2783448603 cites W2120457969 @default.
• W2783448603 cites W2159999899 @default.
• W2783448603 cites W2168360127 @default.
• W2783448603 doi "https://doi.org/10.1016/j.ejvs.2017.12.011" @default.
• W2783448603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29352651" @default.
• W2783448603 hasPublicationYear "2018" @default.
• W2783448603 type Work @default.
• W2783448603 sameAs 2783448603 @default.
• W2783448603 citedByCount "3" @default.
• W2783448603 countsByYear W27834486032019 @default.
• W2783448603 countsByYear W27834486032020 @default.
• W2783448603 countsByYear W27834486032021 @default.
• W2783448603 crossrefType "journal-article" @default.
• W2783448603 hasAuthorship W2783448603A5027008477 @default.
• W2783448603 hasAuthorship W2783448603A5029041534 @default.
• W2783448603 hasAuthorship W2783448603A5044775777 @default.
• W2783448603 hasAuthorship W2783448603A5080447163 @default.
• W2783448603 hasAuthorship W2783448603A5083472385 @default.
• W2783448603 hasBestOaLocation W27834486031 @default.
• W2783448603 hasConcept C127413603 @default.
• W2783448603 hasConcept C131631996 @default.
• W2783448603 hasConcept C141071460 @default.
• W2783448603 hasConcept C142724271 @default.
• W2783448603 hasConcept C189942447 @default.
• W2783448603 hasConcept C198826908 @default.
• W2783448603 hasConcept C2776268601 @default.
• W2783448603 hasConcept C2780007613 @default.
• W2783448603 hasConcept C2781267111 @default.
• W2783448603 hasConcept C42360764 @default.
• W2783448603 hasConcept C71924100 @default.
• W2783448603 hasConceptScore W2783448603C127413603 @default.
• W2783448603 hasConceptScore W2783448603C131631996 @default.
• W2783448603 hasConceptScore W2783448603C141071460 @default.
• W2783448603 hasConceptScore W2783448603C142724271 @default.
• W2783448603 hasConceptScore W2783448603C189942447 @default.
• W2783448603 hasConceptScore W2783448603C198826908 @default.
• W2783448603 hasConceptScore W2783448603C2776268601 @default.
• W2783448603 hasConceptScore W2783448603C2780007613 @default.
• W2783448603 hasConceptScore W2783448603C2781267111 @default.
• W2783448603 hasConceptScore W2783448603C42360764 @default.
• W2783448603 hasConceptScore W2783448603C71924100 @default.
• W2783448603 hasIssue "3" @default.
• W2783448603 hasLocation W27834486031 @default.
• W2783448603 hasLocation W27834486032 @default.
• W2783448603 hasLocation W27834486033 @default.
• W2783448603 hasLocation W27834486034 @default.
• W2783448603 hasOpenAccess W2783448603 @default.
• W2783448603 hasPrimaryLocation W27834486031 @default.
• W2783448603 hasRelatedWork W2003938723 @default.
• W2783448603 hasRelatedWork W2016904036 @default.
• W2783448603 hasRelatedWork W2029625708 @default.
• W2783448603 hasRelatedWork W2047967234 @default.
• W2783448603 hasRelatedWork W2118496982 @default.
• W2783448603 hasRelatedWork W2133242615 @default.
• W2783448603 hasRelatedWork W2415725998 @default.
• W2783448603 hasRelatedWork W2417910727 @default.
• W2783448603 hasRelatedWork W2439875401 @default.
• W2783448603 hasRelatedWork W2525756941 @default.
• W2783448603 hasVolume "55" @default.
• W2783448603 isParatext "false" @default.
• W2783448603 isRetracted "false" @default.
• W2783448603 magId "2783448603" @default.
• W2783448603 workType "article" @default.