FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783452768> ?p ?o ?g. }

• W2783452768 abstract "Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nuclear transfer (SCNT) to generate genetically engineered (GE) pigs carrying site-specific modifications. Although possible, this approach is extremely inefficient and GE pigs born through this method often presented developmental defects associated with the cloning process. Advancement in the gene-editing systems such as Zinc-Finger Nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and the Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) system have dramatically increased the efficiency of producing GE pigs. These gene-editing systems, specifically engineered endonucleases, are based on inducing double-stranded breaks (DSBs) at a specific location, and then site-specific modifications can be introduced through one of the two DNA repair pathways: non-homologous end joining (NHEJ) or homology direct repair (HDR). Random insertions or deletions (indels) can be introduced through NHEJ and specific nucleotide sequences can be introduced through HDR, if donor DNA is provided. Use of these engineered endonucleases provides a higher success in genetic modifications, multiallelic modification of the genome, and an opportunity to introduce site-specific modifications during embryogenesis, thus bypassing the need of SCNT in GE pig production. This review will provide a historical prospective of GE pig production and examples of how the gene-editing system, led by engineered endonucleases, have improved GE pig production. We will also present some of our current progress related to the optimal use of CRISPR/Cas9 system during embryogenesis." @default.
• W2783452768 created "2018-01-26" @default.
• W2783452768 creator A5024184340 @default.
• W2783452768 creator A5046922025 @default.
• W2783452768 creator A5090080677 @default.
• W2783452768 date "2018-01-29" @default.
• W2783452768 modified "2023-10-14" @default.
• W2783452768 title "Use of gene-editing technology to introduce targeted modifications in pigs" @default.
• W2783452768 cites W1088099744 @default.
• W2783452768 cites W1128116721 @default.
• W2783452768 cites W1509485701 @default.
• W2783452768 cites W1531234455 @default.
• W2783452768 cites W1538506308 @default.
• W2783452768 cites W1587462939 @default.
• W2783452768 cites W1616809472 @default.
• W2783452768 cites W19138002 @default.
• W2783452768 cites W1950503156 @default.
• W2783452768 cites W1968283357 @default.
• W2783452768 cites W1968458490 @default.
• W2783452768 cites W1969495999 @default.
• W2783452768 cites W1970894097 @default.
• W2783452768 cites W1972361954 @default.
• W2783452768 cites W1976508887 @default.
• W2783452768 cites W1979411388 @default.
• W2783452768 cites W1988977149 @default.
• W2783452768 cites W1989836279 @default.
• W2783452768 cites W1991266458 @default.
• W2783452768 cites W1992525863 @default.
• W2783452768 cites W1994831934 @default.
• W2783452768 cites W1996652202 @default.
• W2783452768 cites W2008147758 @default.
• W2783452768 cites W2009582877 @default.
• W2783452768 cites W2009626008 @default.
• W2783452768 cites W2012552985 @default.
• W2783452768 cites W2013884618 @default.
• W2783452768 cites W2015718504 @default.
• W2783452768 cites W2019670881 @default.
• W2783452768 cites W2021749433 @default.
• W2783452768 cites W2022835856 @default.
• W2783452768 cites W2023895619 @default.
• W2783452768 cites W2024521921 @default.
• W2783452768 cites W2028228274 @default.
• W2783452768 cites W2032002636 @default.
• W2783452768 cites W2033735287 @default.
• W2783452768 cites W2034533801 @default.
• W2783452768 cites W2042119550 @default.
• W2783452768 cites W2042257807 @default.
• W2783452768 cites W2045435533 @default.
• W2783452768 cites W2047032063 @default.
• W2783452768 cites W2049582819 @default.
• W2783452768 cites W2055062197 @default.
• W2783452768 cites W2059727398 @default.
• W2783452768 cites W2063148079 @default.
• W2783452768 cites W2063804958 @default.
• W2783452768 cites W2064815984 @default.
• W2783452768 cites W2068496041 @default.
• W2783452768 cites W2070721957 @default.
• W2783452768 cites W2072299899 @default.
• W2783452768 cites W2072318929 @default.
• W2783452768 cites W2072600684 @default.
• W2783452768 cites W2073873559 @default.
• W2783452768 cites W2075679174 @default.
• W2783452768 cites W2077659966 @default.
• W2783452768 cites W2078308455 @default.
• W2783452768 cites W2078904081 @default.
• W2783452768 cites W2080885049 @default.
• W2783452768 cites W2083509286 @default.
• W2783452768 cites W2087326716 @default.
• W2783452768 cites W2088135095 @default.
• W2783452768 cites W2088651217 @default.
• W2783452768 cites W2089929813 @default.
• W2783452768 cites W2090701628 @default.
• W2783452768 cites W2090787765 @default.
• W2783452768 cites W2098640007 @default.
• W2783452768 cites W2109819364 @default.
• W2783452768 cites W2112006537 @default.
• W2783452768 cites W2113022445 @default.
• W2783452768 cites W2115804728 @default.
• W2783452768 cites W2116565356 @default.
• W2783452768 cites W2118746294 @default.
• W2783452768 cites W2124369979 @default.
• W2783452768 cites W2124518802 @default.
• W2783452768 cites W2128877497 @default.
• W2783452768 cites W2129602359 @default.
• W2783452768 cites W2139229198 @default.
• W2783452768 cites W2153163241 @default.
• W2783452768 cites W2157361557 @default.
• W2783452768 cites W2157425075 @default.
• W2783452768 cites W2161488472 @default.
• W2783452768 cites W2162983277 @default.
• W2783452768 cites W2163649189 @default.
• W2783452768 cites W2165869860 @default.
• W2783452768 cites W2167380395 @default.
• W2783452768 cites W2168245648 @default.
• W2783452768 cites W2168460045 @default.
• W2783452768 cites W2168504426 @default.
• W2783452768 cites W2169769306 @default.
• W2783452768 cites W2170039745 @default.
• W2783452768 cites W2175791986 @default.
• W2783452768 cites W2288966576 @default.

• next