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- W2783507460 abstract "The PCR procedure to detect Aeromonas schubertii bacteria, which causes white inclusions in internal organs of snakehead fish, was carried out and standardized. The primer pairs Schubertii-16S- (UF-2) F and Schubertii-16S- (UF-2) R (Demarta et al., 1999) were used to amplify the 16s rDNA gene for A. schubertii with a 322-bp PCR product was used in order to shorten the detection time and specific to the causative agent. The optimized PCR procedure includes 1 mM MgCl 2, 1U Taq DNA polymerase and 0.2 mM dNTPs. The detection limit of this PCR protocol is approximately of 30 ng DNA. The specificity of the primer pair is determined to detect only A. schubertii bacteria without detection of some common bacterial pathogens in aquaculture such as Streptococcus agalactiae, Aeromonas hydrophila, Vibrio parahaemolyticus, Edwardsiella ictaluri and Flavobactertium columnare." @default.
- W2783507460 created "2018-01-26" @default.
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- W2783507460 date "2017-01-01" @default.
- W2783507460 modified "2023-09-25" @default.
- W2783507460 title "Phát hiện vi khuẩn Aeromonas schubertii gây đốm trắng ở nội quan cá lóc (Channa striata) bằng phương pháp PCR" @default.
- W2783507460 doi "https://doi.org/10.22144/ctu.jvn.2017.154" @default.
- W2783507460 hasPublicationYear "2017" @default.
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