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- W2783608542 abstract "ABSTRACTChicory (Cichorium spp.) is a valuable vegetable crop worldwide for its edible leaves and for the production of coffee substitutes from roots. Agrobacterium rhizogenes-mediated genetic transformation of two species of chicory (C. intybus and C. endivia) was investigated using Agrobacterium strain K599 harbouring p35SGFPGUS+ plasmid and two types of explants: leave and leaf stalks. This Agrobacterium strain proved to be competent in the transformation with transformation rate about (23.1%) in leaf explants of C. intybus. However, the transformation rate with C. endivia was much lower (3.6%). Moreover, the hairy roots appeared from different infection sites of the leaf explants. Several hairy roots of the two species were acquired, out of them 11 clones (C. intybus) and two clones (C. endivia) were selected due to their fast-growing character. Growth of hairy roots was determined on the basis of total root biomass accumulation. It was found that the liquid MS-basal medium seems to be the most suitab..." @default.
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- W2783608542 date "2018-01-09" @default.
- W2783608542 modified "2023-09-24" @default.
- W2783608542 title "Agrobacterium rhizogenes-mediated genetic transformation in Cichorium spp.: hairy root production, inulin and total phenolic compounds analysis" @default.
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- W2783608542 doi "https://doi.org/10.1080/14620316.2017.1420429" @default.
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