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- W2783649616 abstract "Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·106. As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·104 Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells." @default.
- W2783649616 created "2018-01-26" @default.
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- W2783649616 date "2018-01-11" @default.
- W2783649616 modified "2023-09-27" @default.
- W2783649616 title "Quantitative ptychographic bio-imaging in the water window" @default.
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- W2783649616 doi "https://doi.org/10.1364/oe.26.001237" @default.
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