FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783754045> ?p ?o ?g. }

• W2783754045 endingPage "941" @default.
• W2783754045 startingPage "929" @default.
• W2783754045 abstract "Overexpression of the proinflammatory cytokine macrophage migration inhibitory factor (MIF) is linked to a number of autoimmune diseases and cancer. MIF production has been correlated to the number of CATT repeats in a microsatellite region upstream of the MIF gene. We have characterized the interaction of pituitary-specific positive transcription factor 1 (Pit-1) with a portion of the MIF promoter region flanking a microsatellite polymorphism (-794 CATT5-8). Using fluorescence anisotropy, we quantified tight complex formation between Pit-1 and an oligonucleotide consisting of eight consecutive CATT repeats (8xCATT) with an apparent Kd of 35 nM. Using competition experiments we found a 23 base pair oligonucleotide with 4xCATT repeats to be the minimum DNA sequence necessary for high affinity interaction with Pit-1. The stoichiometry of the Pit-1 DNA interaction was determined to be 2:1 and binding is cooperative in nature. We subsequently structurally characterized the complex and discovered a completely novel binding mode for Pit-1 in contrast to previously described Pit-1 complex structures. The affinity of Pit-1 for the CATT target sequence was found to be highly dependent on cooperativity. This work lays the groundwork for understanding transcriptional regulation of MIF and pursuing Pit-1 as a therapeutic target to treat MIF-mediated inflammatory disorders." @default.
• W2783754045 created "2018-01-26" @default.
• W2783754045 creator A5009877868 @default.
• W2783754045 creator A5089403623 @default.
• W2783754045 date "2017-11-23" @default.
• W2783754045 modified "2023-10-03" @default.
• W2783754045 title "Biochemical and structural characterization of a novel cooperative binding mode by Pit-1 with CATT repeats in the macrophage migration inhibitory factor promoter" @default.
• W2783754045 cites W1499128958 @default.
• W2783754045 cites W1547617108 @default.
• W2783754045 cites W1574734561 @default.
• W2783754045 cites W1863680419 @default.
• W2783754045 cites W1969230829 @default.
• W2783754045 cites W1972792790 @default.
• W2783754045 cites W1974433996 @default.
• W2783754045 cites W1976260744 @default.
• W2783754045 cites W1983424335 @default.
• W2783754045 cites W1985020303 @default.
• W2783754045 cites W1987910138 @default.
• W2783754045 cites W1989832114 @default.
• W2783754045 cites W1990097058 @default.
• W2783754045 cites W1991791352 @default.
• W2783754045 cites W1997641930 @default.
• W2783754045 cites W2002061339 @default.
• W2783754045 cites W2004377868 @default.
• W2783754045 cites W2004458209 @default.
• W2783754045 cites W2006096547 @default.
• W2783754045 cites W2010950394 @default.
• W2783754045 cites W2020733688 @default.
• W2783754045 cites W2024970329 @default.
• W2783754045 cites W2028747292 @default.
• W2783754045 cites W2030165987 @default.
• W2783754045 cites W2030775866 @default.
• W2783754045 cites W2034454625 @default.
• W2783754045 cites W2034892963 @default.
• W2783754045 cites W2034997602 @default.
• W2783754045 cites W2038629196 @default.
• W2783754045 cites W2040336985 @default.
• W2783754045 cites W2044790215 @default.
• W2783754045 cites W2047466377 @default.
• W2783754045 cites W2049878642 @default.
• W2783754045 cites W2052764235 @default.
• W2783754045 cites W2055853358 @default.
• W2783754045 cites W2062876308 @default.
• W2783754045 cites W2063673269 @default.
• W2783754045 cites W2065214715 @default.
• W2783754045 cites W2070808479 @default.
• W2783754045 cites W2074631079 @default.
• W2783754045 cites W2079836771 @default.
• W2783754045 cites W2092058412 @default.
• W2783754045 cites W2093906255 @default.
• W2783754045 cites W2097955448 @default.
• W2783754045 cites W2103487435 @default.
• W2783754045 cites W2104686984 @default.
• W2783754045 cites W2108921801 @default.
• W2783754045 cites W2110808180 @default.
• W2783754045 cites W2112209654 @default.
• W2783754045 cites W2113904137 @default.
• W2783754045 cites W2115694090 @default.
• W2783754045 cites W2117519640 @default.
• W2783754045 cites W2121264387 @default.
• W2783754045 cites W2122994276 @default.
• W2783754045 cites W2123156076 @default.
• W2783754045 cites W2124762989 @default.
• W2783754045 cites W2125068550 @default.
• W2783754045 cites W2134692291 @default.
• W2783754045 cites W2136909852 @default.
• W2783754045 cites W2142745453 @default.
• W2783754045 cites W2146042781 @default.
• W2783754045 cites W2147339635 @default.
• W2783754045 cites W2147949826 @default.
• W2783754045 cites W2154714625 @default.
• W2783754045 cites W2160898059 @default.
• W2783754045 cites W2165195703 @default.
• W2783754045 cites W2168974130 @default.
• W2783754045 cites W2172582324 @default.
• W2783754045 cites W2180229411 @default.
• W2783754045 cites W2239468437 @default.
• W2783754045 cites W2267127857 @default.
• W2783754045 cites W2267370236 @default.
• W2783754045 cites W2267841403 @default.
• W2783754045 cites W2313088239 @default.
• W2783754045 cites W2516960518 @default.
• W2783754045 cites W2603617582 @default.
• W2783754045 cites W2607174456 @default.
• W2783754045 cites W4213055769 @default.
• W2783754045 cites W4246645317 @default.
• W2783754045 doi "https://doi.org/10.1093/nar/gkx1183" @default.
• W2783754045 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5778499" @default.
• W2783754045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29186613" @default.
• W2783754045 hasPublicationYear "2017" @default.
• W2783754045 type Work @default.
• W2783754045 sameAs 2783754045 @default.
• W2783754045 citedByCount "6" @default.
• W2783754045 countsByYear W27837540452018 @default.
• W2783754045 countsByYear W27837540452019 @default.
• W2783754045 countsByYear W27837540452021 @default.
• W2783754045 countsByYear W27837540452023 @default.
• W2783754045 crossrefType "journal-article" @default.

• next