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- W2783921485 abstract "Ca2+ influx triggers the release of synaptic vesicles at the presynaptic active zone (AZ). A quantitative characterization of presynaptic Ca2+ signaling is critical for understanding synaptic transmission. However, this has remained challenging to establish at the required resolution. Here, we employ confocal and stimulated emission depletion (STED) microscopy to quantify the number (20-330) and arrangement (mostly linear 70 nm × 100-600 nm clusters) of Ca2+ channels at AZs of mouse cochlear inner hair cells (IHCs). Establishing STED Ca2+ imaging, we analyze presynaptic Ca2+ signals at the nanometer scale and find confined elongated Ca2+ domains at normal IHC AZs, whereas Ca2+ domains are spatially spread out at the AZs of bassoon-deficient IHCs. Performing 2D-STED fluorescence lifetime analysis, we arrive at estimates of the Ca2+ concentrations at stimulated IHC AZs of on average 25 µM. We propose that IHCs form bassoon-dependent presynaptic Ca2+-channel clusters of similar density but scalable length, thereby varying the number of Ca2+ channels amongst individual AZs." @default.
- W2783921485 created "2018-01-26" @default.
- W2783921485 creator A5030613973 @default.
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- W2783921485 date "2018-01-18" @default.
- W2783921485 modified "2023-10-16" @default.
- W2783921485 title "Quantitative optical nanophysiology of Ca2+ signaling at inner hair cell active zones" @default.
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- W2783921485 doi "https://doi.org/10.1038/s41467-017-02612-y" @default.
- W2783921485 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5773603" @default.
- W2783921485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29348575" @default.
- W2783921485 hasPublicationYear "2018" @default.
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