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- W2783998688 abstract "Short hairpin RNA (shRNA) screens are powerful tools to probe genetic dependencies in loss-of-function studies, such as the identification of therapeutic targets in cancer research. Lentivirally delivered shRNAs embedded in endogenous microRNA contexts (shRNAmiRs) mediate efficient long-term suppression of target genes suitable for numerous experimental contexts and clinical applications. Here, an easy-to-use laboratory protocol is described, covering the design and pooled assembly of focused shRNAmiR libraries into an optimized, Tet-inducible all-in-one lentiviral vector, packaging of viral particles, followed by retrieval and quantification of hairpin sequences after cellular DNA-recovery. Starting from a gene list to the identification of hits, the protocol enables shRNA screens within 6 weeks." @default.
- W2783998688 created "2018-01-26" @default.
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- W2783998688 date "2018-02-01" @default.
- W2783998688 modified "2023-09-26" @default.
- W2783998688 title "Pooled Generation of Lentiviral Tetracycline-Regulated microRNA Embedded Short Hairpin RNA Libraries" @default.
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- W2783998688 doi "https://doi.org/10.1089/hgtb.2017.182" @default.
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- W2783998688 hasPublicationYear "2018" @default.
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