FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2784139075> ?p ?o ?g. }

• W2784139075 endingPage "704" @default.
• W2784139075 startingPage "697" @default.
• W2784139075 abstract "Abstract BACKGROUND Lipoprotein-associated phospholipase A2 (Lp-PLA2), an enzyme associated with inflammation, is used as a biomarker for cardiovascular disease risk. Both the concentration and activity of Lp-PLA2 have been shown to be clinically relevant. However, there is a discordance between the serum concentration of Lp-PLA2 measured by the standard ELISA-based immunoassays and the activity of this enzyme, leading to substantial discordance in risk categorization depending on assay format. METHODS We developed 2 LC-MS/MS–based assays to quantify serum Lp-PLA2 activity (multiple reaction monitoring detection of product) and concentration [stable isotope standards and capture by antipeptide antibody (SISCAPA) immunoaffinity], and we investigated their correlation to commercially offered colorimetric activity and immunometric concentrations assays. Associations between Lp-PLA2 and lipoproteins and the effect of selected detergents in liberating Lp-PLA2 were evaluated by use of immunoprecipitation and Western blot analyses. RESULTS Serum Lp-PLA2 concentrations measured by quantitative SISCAPA-mass spectrometry were substantially higher than concentrations typically measured by immunoassay and showed an improved agreement with Lp-PLA2 activity. With detergents, liberation of Lp-PLA2 from lipoprotein complexes dramatically increased the amount of protein detected by immunoassay and improved the agreement with activity measurements. CONCLUSIONS Quantitative analysis of Lp-PLA2 concentration and activity by LC-MS/MS assays provided key insight into resolving the well-documented discordance between Lp-PLA2 concentration (determined by immunoassay) and activity. Quantitative detection of Lp-PLA2 by immunoassay appears to be strongly inhibited by interaction of Lp-PLA2 with lipoprotein. Together, the results illustrate the advantages of quantitative LC-MS/MS for measurement of Lp-PLA2 concentration (by SISCAPA) and activity (by direct product detection)." @default.
• W2784139075 created "2018-01-26" @default.
• W2784139075 creator A5016523391 @default.
• W2784139075 creator A5024393746 @default.
• W2784139075 creator A5049647087 @default.
• W2784139075 creator A5067548750 @default.
• W2784139075 creator A5072804501 @default.
• W2784139075 creator A5080913759 @default.
• W2784139075 date "2018-04-01" @default.
• W2784139075 modified "2023-10-14" @default.
• W2784139075 title "Measurement of Lipoprotein-Associated Phospholipase A2 by Use of 3 Different Methods: Exploration of Discordance between ELISA and Activity Assays" @default.
• W2784139075 cites W1485296430 @default.
• W2784139075 cites W1547302919 @default.
• W2784139075 cites W1648812418 @default.
• W2784139075 cites W1889923984 @default.
• W2784139075 cites W1979677540 @default.
• W2784139075 cites W1996248878 @default.
• W2784139075 cites W2020220432 @default.
• W2784139075 cites W2035037428 @default.
• W2784139075 cites W2056296858 @default.
• W2784139075 cites W2061315559 @default.
• W2784139075 cites W2077003710 @default.
• W2784139075 cites W2080664733 @default.
• W2784139075 cites W2095597467 @default.
• W2784139075 cites W2095793137 @default.
• W2784139075 cites W2101207877 @default.
• W2784139075 cites W2105372376 @default.
• W2784139075 cites W2105378753 @default.
• W2784139075 cites W2137904973 @default.
• W2784139075 cites W2152068506 @default.
• W2784139075 cites W2156616929 @default.
• W2784139075 cites W2167033601 @default.
• W2784139075 cites W2178622146 @default.
• W2784139075 cites W2181731376 @default.
• W2784139075 cites W2222845405 @default.
• W2784139075 cites W2327470041 @default.
• W2784139075 doi "https://doi.org/10.1373/clinchem.2017.279752" @default.
• W2784139075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29321128" @default.
• W2784139075 hasPublicationYear "2018" @default.
• W2784139075 type Work @default.
• W2784139075 sameAs 2784139075 @default.
• W2784139075 citedByCount "24" @default.
• W2784139075 countsByYear W27841390752018 @default.
• W2784139075 countsByYear W27841390752019 @default.
• W2784139075 countsByYear W27841390752020 @default.
• W2784139075 countsByYear W27841390752021 @default.
• W2784139075 countsByYear W27841390752022 @default.
• W2784139075 countsByYear W27841390752023 @default.
• W2784139075 crossrefType "journal-article" @default.
• W2784139075 hasAuthorship W2784139075A5016523391 @default.
• W2784139075 hasAuthorship W2784139075A5024393746 @default.
• W2784139075 hasAuthorship W2784139075A5049647087 @default.
• W2784139075 hasAuthorship W2784139075A5067548750 @default.
• W2784139075 hasAuthorship W2784139075A5072804501 @default.
• W2784139075 hasAuthorship W2784139075A5080913759 @default.
• W2784139075 hasBestOaLocation W27841390751 @default.
• W2784139075 hasConcept C104317684 @default.
• W2784139075 hasConcept C159654299 @default.
• W2784139075 hasConcept C181199279 @default.
• W2784139075 hasConcept C185592680 @default.
• W2784139075 hasConcept C203014093 @default.
• W2784139075 hasConcept C2776104379 @default.
• W2784139075 hasConcept C2776415932 @default.
• W2784139075 hasConcept C2776551241 @default.
• W2784139075 hasConcept C2776888444 @default.
• W2784139075 hasConcept C2777034527 @default.
• W2784139075 hasConcept C2778163477 @default.
• W2784139075 hasConcept C2780072125 @default.
• W2784139075 hasConcept C2780420688 @default.
• W2784139075 hasConcept C43617362 @default.
• W2784139075 hasConcept C55493867 @default.
• W2784139075 hasConcept C86803240 @default.
• W2784139075 hasConcept C95986675 @default.
• W2784139075 hasConceptScore W2784139075C104317684 @default.
• W2784139075 hasConceptScore W2784139075C159654299 @default.
• W2784139075 hasConceptScore W2784139075C181199279 @default.
• W2784139075 hasConceptScore W2784139075C185592680 @default.
• W2784139075 hasConceptScore W2784139075C203014093 @default.
• W2784139075 hasConceptScore W2784139075C2776104379 @default.
• W2784139075 hasConceptScore W2784139075C2776415932 @default.
• W2784139075 hasConceptScore W2784139075C2776551241 @default.
• W2784139075 hasConceptScore W2784139075C2776888444 @default.
• W2784139075 hasConceptScore W2784139075C2777034527 @default.
• W2784139075 hasConceptScore W2784139075C2778163477 @default.
• W2784139075 hasConceptScore W2784139075C2780072125 @default.
• W2784139075 hasConceptScore W2784139075C2780420688 @default.
• W2784139075 hasConceptScore W2784139075C43617362 @default.
• W2784139075 hasConceptScore W2784139075C55493867 @default.
• W2784139075 hasConceptScore W2784139075C86803240 @default.
• W2784139075 hasConceptScore W2784139075C95986675 @default.
• W2784139075 hasIssue "4" @default.
• W2784139075 hasLocation W27841390751 @default.
• W2784139075 hasLocation W27841390752 @default.
• W2784139075 hasOpenAccess W2784139075 @default.
• W2784139075 hasPrimaryLocation W27841390751 @default.
• W2784139075 hasRelatedWork W1968516178 @default.
• W2784139075 hasRelatedWork W1997102345 @default.
• W2784139075 hasRelatedWork W2032972268 @default.

• next