Matches in SemOpenAlex for { <https://semopenalex.org/work/W2784284186> ?p ?o ?g. }
- W2784284186 abstract "Mapping the protein-protein interaction (PPi) landscape is of critical importance to furthering our understanding how cells and organisms function. Optogenetic methods, that is, approaches that utilize genetically encoded fluorophores or fluorogenic enzyme reactions, uniquely enable the visualization of biochemical phenomena in live cells with high spatial and temporal accuracy. Applying optogenetic methods to the detection of PPis requires the engineering of protein-based systems in which an optical signal undergoes a substantial change when the two proteins of interest interact. In recent years, researchers have developed a number of creative and effective optogenetic methods that achieve this goal, and used them to further elaborate our map of the PPi landscape. In this review, we provide an introduction to the general principles of optogenetic PPi detection, and then provide a number of representative examples of how these principles have been applied. We have organized this review by categorizing methods based on whether the signal generated is reversible or irreversible in nature, and whether the signal is localized or nonlocalized at the subcellular site of the PPi. We discuss these techniques giving both their benefits and drawbacks to enable rational choices about their potential use. This article is categorized under: Laboratory Methods and Technologies > Imaging Laboratory Methods and Technologies > Macromolecular Interactions, Methods Analytical and Computational Methods > Analytical Methods." @default.
- W2784284186 created "2018-01-26" @default.
- W2784284186 creator A5065727681 @default.
- W2784284186 creator A5074910375 @default.
- W2784284186 date "2018-01-15" @default.
- W2784284186 modified "2023-10-16" @default.
- W2784284186 title "Surveying the landscape of optogenetic methods for detection of protein–protein interactions" @default.
- W2784284186 cites W1591311438 @default.
- W2784284186 cites W189512067 @default.
- W2784284186 cites W1959110222 @default.
- W2784284186 cites W1964049424 @default.
- W2784284186 cites W1965035332 @default.
- W2784284186 cites W1965169361 @default.
- W2784284186 cites W1965215154 @default.
- W2784284186 cites W1969103349 @default.
- W2784284186 cites W1970711298 @default.
- W2784284186 cites W1970877840 @default.
- W2784284186 cites W1972059213 @default.
- W2784284186 cites W1972145831 @default.
- W2784284186 cites W1972919132 @default.
- W2784284186 cites W1973835213 @default.
- W2784284186 cites W1977186059 @default.
- W2784284186 cites W1979451155 @default.
- W2784284186 cites W1979539207 @default.
- W2784284186 cites W1983621817 @default.
- W2784284186 cites W1984216534 @default.
- W2784284186 cites W1984436335 @default.
- W2784284186 cites W1984473506 @default.
- W2784284186 cites W1987054851 @default.
- W2784284186 cites W1989100749 @default.
- W2784284186 cites W1990315216 @default.
- W2784284186 cites W1992583467 @default.
- W2784284186 cites W2001796296 @default.
- W2784284186 cites W2003702049 @default.
- W2784284186 cites W2003725493 @default.
- W2784284186 cites W2017196830 @default.
- W2784284186 cites W2019248292 @default.
- W2784284186 cites W2022752974 @default.
- W2784284186 cites W2023623478 @default.
- W2784284186 cites W2026012812 @default.
- W2784284186 cites W2026206754 @default.
- W2784284186 cites W2028309801 @default.
- W2784284186 cites W2037371605 @default.
- W2784284186 cites W2037611736 @default.
- W2784284186 cites W2037997125 @default.
- W2784284186 cites W2040102227 @default.
- W2784284186 cites W2041914510 @default.
- W2784284186 cites W2042161945 @default.
- W2784284186 cites W2045006795 @default.
- W2784284186 cites W2054473817 @default.
- W2784284186 cites W2055045552 @default.
- W2784284186 cites W2055755990 @default.
- W2784284186 cites W2059536603 @default.
- W2784284186 cites W2069330317 @default.
- W2784284186 cites W2070176786 @default.
- W2784284186 cites W2070375075 @default.
- W2784284186 cites W2070391037 @default.
- W2784284186 cites W2070937252 @default.
- W2784284186 cites W2071688684 @default.
- W2784284186 cites W2079416470 @default.
- W2784284186 cites W2080874961 @default.
- W2784284186 cites W2082359973 @default.
- W2784284186 cites W2083288707 @default.
- W2784284186 cites W2085961271 @default.
- W2784284186 cites W2091804920 @default.
- W2784284186 cites W2096211374 @default.
- W2784284186 cites W2097055018 @default.
- W2784284186 cites W2097248196 @default.
- W2784284186 cites W2097461591 @default.
- W2784284186 cites W2103019017 @default.
- W2784284186 cites W2105537485 @default.
- W2784284186 cites W2107382027 @default.
- W2784284186 cites W2110193578 @default.
- W2784284186 cites W2113146347 @default.
- W2784284186 cites W2113974479 @default.
- W2784284186 cites W2119004056 @default.
- W2784284186 cites W2121243961 @default.
- W2784284186 cites W2122260535 @default.
- W2784284186 cites W2123058210 @default.
- W2784284186 cites W2123924700 @default.
- W2784284186 cites W2126057063 @default.
- W2784284186 cites W2127710713 @default.
- W2784284186 cites W2129163530 @default.
- W2784284186 cites W2129675152 @default.
- W2784284186 cites W2130229792 @default.
- W2784284186 cites W2130511335 @default.
- W2784284186 cites W2135695731 @default.
- W2784284186 cites W2139277416 @default.
- W2784284186 cites W2143937232 @default.
- W2784284186 cites W2144147853 @default.
- W2784284186 cites W2152439945 @default.
- W2784284186 cites W2157129565 @default.
- W2784284186 cites W2159759082 @default.
- W2784284186 cites W2161027891 @default.
- W2784284186 cites W2161768325 @default.
- W2784284186 cites W2161998646 @default.
- W2784284186 cites W2162929095 @default.
- W2784284186 cites W2171008539 @default.
- W2784284186 cites W2226712470 @default.
- W2784284186 cites W2300242283 @default.