Matches in SemOpenAlex for { <https://semopenalex.org/work/W2784292212> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2784292212 abstract "Med6 protein (Med6p) is a hallmark component of evolutionarily conserved Mediator complexes, and the genuine role of Med6p in Mediator functions remains elusive. For the functional analysis of Saccharomyces cerevisiae Med6p (scMed6p), we generated a series of scMed6p mutants harboring a small internal deletion. Genetic analysis of these mutants revealed that three regions (amino acids 33–42 (Δ2), 125–134 (Δ5), and 157–166 (Δ6)) of scMed6p are required for cell viability and are located at highly conserved regions of Med6 homologs. Notably, the Med6p-Δ2 mutant was barely detectable in whole-cell extracts and purified Mediator, suggesting a loss of Mediator association and concurrent rapid degradation. Consistent with this, the recombinant forms of Med6p having these mutations partially (Δ2) restore or fail (Δ5 and Δ6) to restore in vitro transcriptional defects caused by temperature-sensitive med6 mutation. In an artificial recruitment assay, Mediator containing a LexA-fused wild-type Med6p or Med6p-Δ5 was recruited to the lexA operator region with TBP and activated reporter gene expression. However, the recruitment of Mediator containing LexA-Med6p-Δ6 to lexA operator region resulted in neither TBP recruitment nor reporter gene expression. This result demonstrates a pivotal role of Med6p in the postrecruitment function of Mediator, which is essential for transcriptional activation by Mediator." @default.
- W2784292212 created "2018-01-26" @default.
- W2784292212 creator A5034386082 @default.
- W2784292212 creator A5051652536 @default.
- W2784292212 date "2018-01-01" @default.
- W2784292212 modified "2023-10-17" @default.
- W2784292212 title "Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex" @default.
- W2784292212 cites W1566478858 @default.
- W2784292212 cites W1808467244 @default.
- W2784292212 cites W1964318436 @default.
- W2784292212 cites W1982161126 @default.
- W2784292212 cites W1992140850 @default.
- W2784292212 cites W1992338259 @default.
- W2784292212 cites W1995399581 @default.
- W2784292212 cites W2006277271 @default.
- W2784292212 cites W2033042898 @default.
- W2784292212 cites W2037145096 @default.
- W2784292212 cites W2039531009 @default.
- W2784292212 cites W2048156032 @default.
- W2784292212 cites W2074479221 @default.
- W2784292212 cites W2078578982 @default.
- W2784292212 cites W2102514436 @default.
- W2784292212 cites W2117484859 @default.
- W2784292212 cites W2119928648 @default.
- W2784292212 cites W2127201083 @default.
- W2784292212 cites W2135453921 @default.
- W2784292212 cites W2140710406 @default.
- W2784292212 cites W2149920358 @default.
- W2784292212 cites W2151849975 @default.
- W2784292212 cites W2153110406 @default.
- W2784292212 cites W2162042193 @default.
- W2784292212 cites W2168974130 @default.
- W2784292212 cites W2170578515 @default.
- W2784292212 cites W2515162953 @default.
- W2784292212 cites W2591796424 @default.
- W2784292212 cites W2611767197 @default.
- W2784292212 doi "https://doi.org/10.1155/2018/6406372" @default.
- W2784292212 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5818915" @default.
- W2784292212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29992056" @default.
- W2784292212 hasPublicationYear "2018" @default.
- W2784292212 type Work @default.
- W2784292212 sameAs 2784292212 @default.
- W2784292212 citedByCount "1" @default.
- W2784292212 crossrefType "journal-article" @default.
- W2784292212 hasAuthorship W2784292212A5034386082 @default.
- W2784292212 hasAuthorship W2784292212A5051652536 @default.
- W2784292212 hasBestOaLocation W27842922121 @default.
- W2784292212 hasConcept C104317684 @default.
- W2784292212 hasConcept C143065580 @default.
- W2784292212 hasConcept C150194340 @default.
- W2784292212 hasConcept C158448853 @default.
- W2784292212 hasConcept C161733203 @default.
- W2784292212 hasConcept C2107291 @default.
- W2784292212 hasConcept C2777576037 @default.
- W2784292212 hasConcept C413212 @default.
- W2784292212 hasConcept C501734568 @default.
- W2784292212 hasConcept C54355233 @default.
- W2784292212 hasConcept C86803240 @default.
- W2784292212 hasConcept C95444343 @default.
- W2784292212 hasConceptScore W2784292212C104317684 @default.
- W2784292212 hasConceptScore W2784292212C143065580 @default.
- W2784292212 hasConceptScore W2784292212C150194340 @default.
- W2784292212 hasConceptScore W2784292212C158448853 @default.
- W2784292212 hasConceptScore W2784292212C161733203 @default.
- W2784292212 hasConceptScore W2784292212C2107291 @default.
- W2784292212 hasConceptScore W2784292212C2777576037 @default.
- W2784292212 hasConceptScore W2784292212C413212 @default.
- W2784292212 hasConceptScore W2784292212C501734568 @default.
- W2784292212 hasConceptScore W2784292212C54355233 @default.
- W2784292212 hasConceptScore W2784292212C86803240 @default.
- W2784292212 hasConceptScore W2784292212C95444343 @default.
- W2784292212 hasFunder F4320322120 @default.
- W2784292212 hasLocation W27842922121 @default.
- W2784292212 hasLocation W27842922122 @default.
- W2784292212 hasLocation W27842922123 @default.
- W2784292212 hasLocation W27842922124 @default.
- W2784292212 hasOpenAccess W2784292212 @default.
- W2784292212 hasPrimaryLocation W27842922121 @default.
- W2784292212 hasRelatedWork W101124211 @default.
- W2784292212 hasRelatedWork W1826456176 @default.
- W2784292212 hasRelatedWork W1968643238 @default.
- W2784292212 hasRelatedWork W2021380639 @default.
- W2784292212 hasRelatedWork W2021674453 @default.
- W2784292212 hasRelatedWork W2072813167 @default.
- W2784292212 hasRelatedWork W2102678326 @default.
- W2784292212 hasRelatedWork W2111958127 @default.
- W2784292212 hasRelatedWork W2460754768 @default.
- W2784292212 hasRelatedWork W2784292212 @default.
- W2784292212 isParatext "false" @default.
- W2784292212 isRetracted "false" @default.
- W2784292212 magId "2784292212" @default.
- W2784292212 workType "article" @default.