Matches in SemOpenAlex for { <https://semopenalex.org/work/W2784926397> ?p ?o ?g. }
- W2784926397 endingPage "178" @default.
- W2784926397 startingPage "171" @default.
- W2784926397 abstract "The final stage of in vitro osteogenic differentiation is characterized by the production of mineral deposits containing calcium cations and inorganic phosphates, which populate the extracellular matrix (ECM) surrounding the cell monolayer. Conventional histological techniques for the assessment of mineralization, such as Von Kossa and Alizarin Red S staining, are end point techniques requiring cell fixation. Moreover, in both cases staining quantitation requires dye extraction, which irreversibly alters the ECM conformation and structure, therefore preventing the use of the sample for further analysis. In this study, the use of tetracycline hydrochloride (TC) is proposed for the nondestructive staining, quantitation, and imaging of mineralizing bone-like nodules in live cultures of human bone marrow mesenchymal stem cells cultured under osteogenic conditions. Overnight administration of TC to living cells was shown not to alter the metabolic activity or the progression of cell differentiation. When applied to differentiating cultures, cell exposure to serial doses of TC was found to produce quantifiable fluorescence emission specifically in osteogenic cultures. Incubation with TC enabled fluorescence imaging of mineralized areas in live cultures and the combination with other fluorophores using appropriate filters. These results demonstrate that serial TC administration over the differentiation time course provides a qualitative and quantitative tool for the monitoring and evaluation of the differentiation process in live cells." @default.
- W2784926397 created "2018-02-02" @default.
- W2784926397 creator A5006784212 @default.
- W2784926397 creator A5018037754 @default.
- W2784926397 creator A5040959976 @default.
- W2784926397 creator A5071710182 @default.
- W2784926397 creator A5081757413 @default.
- W2784926397 creator A5083593340 @default.
- W2784926397 date "2018-03-01" @default.
- W2784926397 modified "2023-10-18" @default.
- W2784926397 title "Live Quantitative Monitoring of Mineral Deposition in Stem Cells Using Tetracycline Hydrochloride" @default.
- W2784926397 cites W132393281 @default.
- W2784926397 cites W1756472966 @default.
- W2784926397 cites W1826407790 @default.
- W2784926397 cites W1918240731 @default.
- W2784926397 cites W1964849148 @default.
- W2784926397 cites W1972318864 @default.
- W2784926397 cites W1976841774 @default.
- W2784926397 cites W1983894674 @default.
- W2784926397 cites W1996016867 @default.
- W2784926397 cites W2004496950 @default.
- W2784926397 cites W2009237943 @default.
- W2784926397 cites W2010134281 @default.
- W2784926397 cites W2018255715 @default.
- W2784926397 cites W2023407069 @default.
- W2784926397 cites W2031403556 @default.
- W2784926397 cites W2031931232 @default.
- W2784926397 cites W2047775069 @default.
- W2784926397 cites W2058370892 @default.
- W2784926397 cites W2066259773 @default.
- W2784926397 cites W2068198133 @default.
- W2784926397 cites W2073151761 @default.
- W2784926397 cites W2077108066 @default.
- W2784926397 cites W2086704116 @default.
- W2784926397 cites W2094646915 @default.
- W2784926397 cites W2099721111 @default.
- W2784926397 cites W2111311106 @default.
- W2784926397 cites W2117503439 @default.
- W2784926397 cites W2128118061 @default.
- W2784926397 cites W2133230912 @default.
- W2784926397 cites W2149073570 @default.
- W2784926397 cites W2150951939 @default.
- W2784926397 cites W2163693454 @default.
- W2784926397 cites W2346184348 @default.
- W2784926397 cites W4253262038 @default.
- W2784926397 doi "https://doi.org/10.1089/ten.tec.2017.0400" @default.
- W2784926397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5865259" @default.
- W2784926397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29353532" @default.
- W2784926397 hasPublicationYear "2018" @default.
- W2784926397 type Work @default.
- W2784926397 sameAs 2784926397 @default.
- W2784926397 citedByCount "20" @default.
- W2784926397 countsByYear W27849263972018 @default.
- W2784926397 countsByYear W27849263972019 @default.
- W2784926397 countsByYear W27849263972020 @default.
- W2784926397 countsByYear W27849263972021 @default.
- W2784926397 countsByYear W27849263972022 @default.
- W2784926397 countsByYear W27849263972023 @default.
- W2784926397 crossrefType "journal-article" @default.
- W2784926397 hasAuthorship W2784926397A5006784212 @default.
- W2784926397 hasAuthorship W2784926397A5018037754 @default.
- W2784926397 hasAuthorship W2784926397A5040959976 @default.
- W2784926397 hasAuthorship W2784926397A5071710182 @default.
- W2784926397 hasAuthorship W2784926397A5081757413 @default.
- W2784926397 hasAuthorship W2784926397A5083593340 @default.
- W2784926397 hasBestOaLocation W27849263971 @default.
- W2784926397 hasConcept C106487976 @default.
- W2784926397 hasConcept C121332964 @default.
- W2784926397 hasConcept C131075544 @default.
- W2784926397 hasConcept C142724271 @default.
- W2784926397 hasConcept C160160445 @default.
- W2784926397 hasConcept C169274487 @default.
- W2784926397 hasConcept C181199279 @default.
- W2784926397 hasConcept C185592680 @default.
- W2784926397 hasConcept C189165786 @default.
- W2784926397 hasConcept C198826908 @default.
- W2784926397 hasConcept C202751555 @default.
- W2784926397 hasConcept C2780923616 @default.
- W2784926397 hasConcept C28328180 @default.
- W2784926397 hasConcept C2908999040 @default.
- W2784926397 hasConcept C2910894859 @default.
- W2784926397 hasConcept C43617362 @default.
- W2784926397 hasConcept C501593827 @default.
- W2784926397 hasConcept C54355233 @default.
- W2784926397 hasConcept C55493867 @default.
- W2784926397 hasConcept C62520636 @default.
- W2784926397 hasConcept C66694092 @default.
- W2784926397 hasConcept C71924100 @default.
- W2784926397 hasConcept C74864618 @default.
- W2784926397 hasConcept C81885089 @default.
- W2784926397 hasConcept C86803240 @default.
- W2784926397 hasConcept C91881484 @default.
- W2784926397 hasConcept C95444343 @default.
- W2784926397 hasConceptScore W2784926397C106487976 @default.
- W2784926397 hasConceptScore W2784926397C121332964 @default.
- W2784926397 hasConceptScore W2784926397C131075544 @default.
- W2784926397 hasConceptScore W2784926397C142724271 @default.
- W2784926397 hasConceptScore W2784926397C160160445 @default.