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• W2784945841 abstract "Background and Purpose Olaparib, rucaparib and niraparib, potent inhibitors of poly(ADP-ribose) polymerase (PARP) are approved as anti-cancer drugs in humans. Considering the previously demonstrated role of PARP in various forms of acute and chronic myocardial injury, we tested the effects of olaparib in in-vitro models of oxidative stress in cardiomyocytes, and in an in vivo model of cardiac transplantation. Experimental Approach H9c2-embryonic rat heart-derived myoblasts pretreated with vehicle or olaparib (10μM) were challenged with either hydrogen peroxide (H2O2) or with glucose oxidase (GOx, which generates H2O2 in the tissue culture medium). Cell viability assays (MTT, lactate dehydrogenase) and Western blotting for PARP and its product, PAR was performed. Heterotopic heart transplantation was performed in Lewis rats; recipients were treated either with vehicle or olaparib (10 mg kg-1). Left ventricular function of transplanted hearts was monitored via a Millar catheter. Multiple gene expression in the graft was measured by qPCR. Key Results Olaparib blocked autoPARylation of PARP1 and attenuated the rapid onset of death in H9c2 cells, induced by H2O2, but did not affect cell death following chronic, prolonged oxidative stress induced by GOx. In rats, after transplantation, left ventricular systolic and diastolic function were improved by olaparib. In the transplanted hearts, olaparib also reduced gene expression for c-jun, caspase-12, catalase, and NADPH oxidase-2. Conclusions and Implications Olaparib protected cardiomyocytes against oxidative stress and improved graft contractility in a rat model of heart transplantation. These findings raise the possibility of repurposing this clinically approved oncology drug, to be used in heart transplantation. Linked Articles This article is part of a themed section on Inventing New Therapies Without Reinventing the Wheel: The Power of Drug Repurposing. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.2/issuetoc" @default.
• W2784945841 created "2018-02-02" @default.
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• W2784945841 date "2017-10-02" @default.
• W2784945841 modified "2023-10-15" @default.
• W2784945841 title "Olaparib protects cardiomyocytes against oxidative stress and improves graft contractility during the early phase after heart transplantation in rats" @default.
• W2784945841 cites W1045337831 @default.
• W2784945841 cites W1499143025 @default.
• W2784945841 cites W1506161763 @default.
• W2784945841 cites W1593555249 @default.
• W2784945841 cites W1596457615 @default.
• W2784945841 cites W1599703185 @default.
• W2784945841 cites W188183793 @default.
• W2784945841 cites W1888470382 @default.
• W2784945841 cites W1964531991 @default.
• W2784945841 cites W1965006127 @default.
• W2784945841 cites W1971954823 @default.
• W2784945841 cites W1974045633 @default.
• W2784945841 cites W1974643497 @default.
• W2784945841 cites W1975255909 @default.
• W2784945841 cites W1975490162 @default.
• W2784945841 cites W1976805974 @default.
• W2784945841 cites W1997119228 @default.
• W2784945841 cites W1999473143 @default.
• W2784945841 cites W2008749028 @default.
• W2784945841 cites W2009781172 @default.
• W2784945841 cites W2015720223 @default.
• W2784945841 cites W2016367295 @default.
• W2784945841 cites W2017171454 @default.
• W2784945841 cites W2018032623 @default.
• W2784945841 cites W2023357993 @default.
• W2784945841 cites W2024876727 @default.
• W2784945841 cites W2035192492 @default.
• W2784945841 cites W2044390268 @default.
• W2784945841 cites W2049696886 @default.
• W2784945841 cites W2052855638 @default.
• W2784945841 cites W2055988887 @default.
• W2784945841 cites W2058340977 @default.
• W2784945841 cites W2059420441 @default.
• W2784945841 cites W2060799619 @default.
• W2784945841 cites W2061298493 @default.
• W2784945841 cites W2066766482 @default.
• W2784945841 cites W2072219150 @default.
• W2784945841 cites W2075399331 @default.
• W2784945841 cites W2076542944 @default.
• W2784945841 cites W2080633955 @default.
• W2784945841 cites W2084060660 @default.
• W2784945841 cites W2084982869 @default.
• W2784945841 cites W2088607098 @default.
• W2784945841 cites W2092358394 @default.
• W2784945841 cites W2093814798 @default.
• W2784945841 cites W2095798342 @default.
• W2784945841 cites W2096541614 @default.
• W2784945841 cites W2105271014 @default.
• W2784945841 cites W2105563644 @default.
• W2784945841 cites W2114344808 @default.
• W2784945841 cites W2116118256 @default.
• W2784945841 cites W2116830457 @default.
• W2784945841 cites W2121092271 @default.
• W2784945841 cites W2128960919 @default.
• W2784945841 cites W2133771754 @default.
• W2784945841 cites W2138797601 @default.
• W2784945841 cites W2139084430 @default.
• W2784945841 cites W2146830757 @default.
• W2784945841 cites W2159534384 @default.
• W2784945841 cites W2163482228 @default.
• W2784945841 cites W2163637636 @default.
• W2784945841 cites W2165010546 @default.
• W2784945841 cites W2165980423 @default.
• W2784945841 cites W2170530467 @default.
• W2784945841 cites W2194756172 @default.
• W2784945841 cites W2196064518 @default.
• W2784945841 cites W2204866603 @default.
• W2784945841 cites W2267910177 @default.
• W2784945841 cites W2296388187 @default.
• W2784945841 cites W2314004473 @default.
• W2784945841 cites W2314558022 @default.
• W2784945841 cites W2346552090 @default.
• W2784945841 cites W2521759723 @default.
• W2784945841 cites W2540412673 @default.
• W2784945841 cites W256522293 @default.
• W2784945841 cites W2569816556 @default.
• W2784945841 cites W2588135595 @default.
• W2784945841 cites W2588341962 @default.
• W2784945841 cites W2610599595 @default.
• W2784945841 cites W2612277616 @default.
• W2784945841 cites W4253095320 @default.
• W2784945841 doi "https://doi.org/10.1111/bph.13983" @default.
• W2784945841 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5758401" @default.
• W2784945841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28806493" @default.

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