FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2785017462> ?p ?o ?g. }

• W2785017462 endingPage "1283" @default.
• W2785017462 startingPage "1274" @default.
• W2785017462 abstract "Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis. Accurate analysis of PG composition provides crucial insights into the bacterium’s cellular functions and responses to external stimuli, but this analysis remains challenging because of various chemical modifications to PG-repeat subunits. We characterized changes to the PG composition of E. faecalis grown as planktonic bacteria and biofilm by developing “stable isotope labeling by amino acids in bacterial culture” (SILAB), optimized for bacterial cultures with incomplete amino acid labeling. This comparative analysis by mass spectrometry was performed by labeling E. faecalis in biofilm with heavy Lys (l-[13C6,2D9,15N2]Lys) and planktonic bacteria with natural abundance l-Lys, then mixing equal amounts of bacteria from each condition, and performing cell wall isolation and mutanolysin digestion necessary for liquid chromatography and mass spectrometry. An analytical method was developed to determine muropeptide abundances using correction factors to compensate for incomplete heavy Lys isotopic enrichment (98.33 ± 0.05%) and incorporation (83.23 ± 1.16%). Forty-seven pairs of PG fragment ions from isolated cell walls of planktonic and biofilm samples were selected for SILAB analysis. We found that the PG in biofilm showed an increased level of PG cross-linking, an increased level of N-deacetylation of GlcNAc, a decreased level of O-acetylation of MurNAc, and an increased number of stem modifications by d,d- and l,d-carboxypeptidases." @default.
• W2785017462 created "2018-02-02" @default.
• W2785017462 creator A5012617076 @default.
• W2785017462 creator A5046639377 @default.
• W2785017462 creator A5048370915 @default.
• W2785017462 creator A5065906325 @default.
• W2785017462 date "2018-02-02" @default.
• W2785017462 modified "2023-10-14" @default.
• W2785017462 title "Peptidoglycan Compositional Analysis of <i>Enterococcus faecalis</i> Biofilm by Stable Isotope Labeling by Amino Acids in a Bacterial Culture" @default.
• W2785017462 cites W1761042522 @default.
• W2785017462 cites W1849957189 @default.
• W2785017462 cites W1967663562 @default.
• W2785017462 cites W1975942496 @default.
• W2785017462 cites W1982705370 @default.
• W2785017462 cites W2001833616 @default.
• W2785017462 cites W2006900192 @default.
• W2785017462 cites W2006986627 @default.
• W2785017462 cites W2008186286 @default.
• W2785017462 cites W2011635923 @default.
• W2785017462 cites W2024799232 @default.
• W2785017462 cites W2031269216 @default.
• W2785017462 cites W2044251513 @default.
• W2785017462 cites W2044562813 @default.
• W2785017462 cites W2045404157 @default.
• W2785017462 cites W2053684580 @default.
• W2785017462 cites W2053830123 @default.
• W2785017462 cites W2058127453 @default.
• W2785017462 cites W2064631610 @default.
• W2785017462 cites W2072815562 @default.
• W2785017462 cites W2076747780 @default.
• W2785017462 cites W2086216302 @default.
• W2785017462 cites W2111616162 @default.
• W2785017462 cites W2118058022 @default.
• W2785017462 cites W2125465873 @default.
• W2785017462 cites W2125934160 @default.
• W2785017462 cites W2133391121 @default.
• W2785017462 cites W2137366286 @default.
• W2785017462 cites W2140425260 @default.
• W2785017462 cites W2140946052 @default.
• W2785017462 cites W2141992377 @default.
• W2785017462 cites W2157162286 @default.
• W2785017462 cites W2162392176 @default.
• W2785017462 cites W2323865636 @default.
• W2785017462 cites W2515809663 @default.
• W2785017462 cites W2516357724 @default.
• W2785017462 cites W2519691041 @default.
• W2785017462 cites W2567324748 @default.
• W2785017462 cites W2581766148 @default.
• W2785017462 cites W2589734778 @default.
• W2785017462 cites W2606179125 @default.
• W2785017462 doi "https://doi.org/10.1021/acs.biochem.7b01207" @default.
• W2785017462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5972391" @default.
• W2785017462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29368511" @default.
• W2785017462 hasPublicationYear "2018" @default.
• W2785017462 type Work @default.
• W2785017462 sameAs 2785017462 @default.
• W2785017462 citedByCount "19" @default.
• W2785017462 countsByYear W27850174622018 @default.
• W2785017462 countsByYear W27850174622020 @default.
• W2785017462 countsByYear W27850174622021 @default.
• W2785017462 countsByYear W27850174622022 @default.
• W2785017462 countsByYear W27850174622023 @default.
• W2785017462 crossrefType "journal-article" @default.
• W2785017462 hasAuthorship W2785017462A5012617076 @default.
• W2785017462 hasAuthorship W2785017462A5046639377 @default.
• W2785017462 hasAuthorship W2785017462A5048370915 @default.
• W2785017462 hasAuthorship W2785017462A5065906325 @default.
• W2785017462 hasBestOaLocation W27850174622 @default.
• W2785017462 hasConcept C125235067 @default.
• W2785017462 hasConcept C185592680 @default.
• W2785017462 hasConcept C19655278 @default.
• W2785017462 hasConcept C2776634448 @default.
• W2785017462 hasConcept C2779489039 @default.
• W2785017462 hasConcept C2779895986 @default.
• W2785017462 hasConcept C43617362 @default.
• W2785017462 hasConcept C515207424 @default.
• W2785017462 hasConcept C523546767 @default.
• W2785017462 hasConcept C54355233 @default.
• W2785017462 hasConcept C55493867 @default.
• W2785017462 hasConcept C58123911 @default.
• W2785017462 hasConcept C86803240 @default.
• W2785017462 hasConcept C89423630 @default.
• W2785017462 hasConceptScore W2785017462C125235067 @default.
• W2785017462 hasConceptScore W2785017462C185592680 @default.
• W2785017462 hasConceptScore W2785017462C19655278 @default.
• W2785017462 hasConceptScore W2785017462C2776634448 @default.
• W2785017462 hasConceptScore W2785017462C2779489039 @default.
• W2785017462 hasConceptScore W2785017462C2779895986 @default.
• W2785017462 hasConceptScore W2785017462C43617362 @default.
• W2785017462 hasConceptScore W2785017462C515207424 @default.
• W2785017462 hasConceptScore W2785017462C523546767 @default.
• W2785017462 hasConceptScore W2785017462C54355233 @default.
• W2785017462 hasConceptScore W2785017462C55493867 @default.
• W2785017462 hasConceptScore W2785017462C58123911 @default.
• W2785017462 hasConceptScore W2785017462C86803240 @default.
• W2785017462 hasConceptScore W2785017462C89423630 @default.
• W2785017462 hasFunder F4320337354 @default.
• W2785017462 hasIssue "7" @default.

• next