FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2785343839> ?p ?o ?g. }

• W2785343839 abstract "Abstract Background Ixodes ricinus is the most important vector of tick-borne-diseases in Europe. A better knowledge of its genome and transcriptome is important for developing control strategies. Previous transcriptomic studies of I. ricinus have focused on gene expression during the blood meal in specific tissues. To obtain a broader picture of changes in gene expression during the blood meal, our study analysed the transcriptome at the level of the whole body for both nymphal and adult ticks. I. ricinus ticks from a highly inbred colony at the University of Neuchâtel were used. We also analysed previously published RNAseq studies to compare the genetic variation between three wild strains and three lab strains, including the strain from Neuchâtel. Results RNA was extracted from whole tick bodies and the cDNA was sequenced, producing 162,872,698 paired-end reads. Our reference transcriptome contained 179,682 contigs, of which 31% were annotated using Trinotate. Gene expression was compared between ticks that differed with respect to stage (nymph, adult), sex (female, male), and feeding status (unfed, partially fed). We found that blood feeding in nymphs and female adult ticks increased the expression of cuticle-associated genes. Using a set of 5,422 single nucleotide polymorphisms to calculate the heterozygosity, we found that the wild tick populations of I. ricinus had much higher levels of heterozygosity than the three lab populations. Conclusion Using high throughput strand-oriented sequencing for whole ticks in different stages and feeding conditions, we obtained a de novo assembly that significantly increased the genomic resources available for I. ricinus . Our study illustrates the importance of analysing the transcriptome at the level of the whole body to gain additional insights into how gene expression changes over the life cycle of an organism. Our comparison of several RNAseq datasets shows the power of transcriptomic data to accurately characterize genetic polymorphism and for comparing different populations or sources of sequencing material." @default.
• W2785343839 created "2018-02-23" @default.
• W2785343839 creator A5004927877 @default.
• W2785343839 creator A5015252334 @default.
• W2785343839 creator A5026690931 @default.
• W2785343839 creator A5045761528 @default.
• W2785343839 creator A5056268649 @default.
• W2785343839 creator A5059911817 @default.
• W2785343839 creator A5076923399 @default.
• W2785343839 creator A5086432078 @default.
• W2785343839 date "2018-01-09" @default.
• W2785343839 modified "2023-09-27" @default.
• W2785343839 title "Whole body transcriptomes and new insights into the biology of the tick Ixodes ricinus" @default.
• W2785343839 cites W1527927437 @default.
• W2785343839 cites W1886649985 @default.
• W2785343839 cites W1895914524 @default.
• W2785343839 cites W1970707135 @default.
• W2785343839 cites W1972185194 @default.
• W2785343839 cites W1984180033 @default.
• W2785343839 cites W2006528936 @default.
• W2785343839 cites W2011657487 @default.
• W2785343839 cites W2012540162 @default.
• W2785343839 cites W2014109817 @default.
• W2785343839 cites W2022493234 @default.
• W2785343839 cites W2027102545 @default.
• W2785343839 cites W2027828573 @default.
• W2785343839 cites W2031342714 @default.
• W2785343839 cites W2045843097 @default.
• W2785343839 cites W2050077513 @default.
• W2785343839 cites W2063206666 @default.
• W2785343839 cites W2065131747 @default.
• W2785343839 cites W2065381296 @default.
• W2785343839 cites W2065669787 @default.
• W2785343839 cites W2069488864 @default.
• W2785343839 cites W2070874919 @default.
• W2785343839 cites W2075774945 @default.
• W2785343839 cites W2076515648 @default.
• W2785343839 cites W2088863268 @default.
• W2785343839 cites W2089973643 @default.
• W2785343839 cites W2094201044 @default.
• W2785343839 cites W2094272156 @default.
• W2785343839 cites W2128452565 @default.
• W2785343839 cites W2131271579 @default.
• W2785343839 cites W2135297744 @default.
• W2785343839 cites W2136145671 @default.
• W2785343839 cites W2138122982 @default.
• W2785343839 cites W2141584209 @default.
• W2785343839 cites W2145204137 @default.
• W2785343839 cites W2147250916 @default.
• W2785343839 cites W2147526198 @default.
• W2785343839 cites W2152770371 @default.
• W2785343839 cites W2155133651 @default.
• W2785343839 cites W2155628349 @default.
• W2785343839 cites W2156125289 @default.
• W2785343839 cites W2162157671 @default.
• W2785343839 cites W2170551349 @default.
• W2785343839 cites W2235243555 @default.
• W2785343839 cites W2281061049 @default.
• W2785343839 cites W2323326409 @default.
• W2785343839 cites W238988786 @default.
• W2785343839 cites W2395791915 @default.
• W2785343839 cites W2502274642 @default.
• W2785343839 cites W2553951693 @default.
• W2785343839 cites W2586486806 @default.
• W2785343839 cites W2604985075 @default.
• W2785343839 cites W4211143140 @default.
• W2785343839 doi "https://doi.org/10.1101/244830" @default.
• W2785343839 hasPublicationYear "2018" @default.
• W2785343839 type Work @default.
• W2785343839 sameAs 2785343839 @default.
• W2785343839 citedByCount "0" @default.
• W2785343839 crossrefType "posted-content" @default.
• W2785343839 hasAuthorship W2785343839A5004927877 @default.
• W2785343839 hasAuthorship W2785343839A5015252334 @default.
• W2785343839 hasAuthorship W2785343839A5026690931 @default.
• W2785343839 hasAuthorship W2785343839A5045761528 @default.
• W2785343839 hasAuthorship W2785343839A5056268649 @default.
• W2785343839 hasAuthorship W2785343839A5059911817 @default.
• W2785343839 hasAuthorship W2785343839A5076923399 @default.
• W2785343839 hasAuthorship W2785343839A5086432078 @default.
• W2785343839 hasBestOaLocation W27853438391 @default.
• W2785343839 hasConcept C104317684 @default.
• W2785343839 hasConcept C143589142 @default.
• W2785343839 hasConcept C150194340 @default.
• W2785343839 hasConcept C162317418 @default.
• W2785343839 hasConcept C180754005 @default.
• W2785343839 hasConcept C18903297 @default.
• W2785343839 hasConcept C2777127260 @default.
• W2785343839 hasConcept C2777923404 @default.
• W2785343839 hasConcept C2778576062 @default.
• W2785343839 hasConcept C2779620486 @default.
• W2785343839 hasConcept C54355233 @default.
• W2785343839 hasConcept C59822182 @default.
• W2785343839 hasConcept C85323577 @default.
• W2785343839 hasConcept C86803240 @default.
• W2785343839 hasConcept C90856448 @default.
• W2785343839 hasConceptScore W2785343839C104317684 @default.
• W2785343839 hasConceptScore W2785343839C143589142 @default.
• W2785343839 hasConceptScore W2785343839C150194340 @default.
• W2785343839 hasConceptScore W2785343839C162317418 @default.

• next