FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2785363467> ?p ?o ?g. }

• W2785363467 abstract "Intracellular pathogenic bacteria such as Salmonella or Shigella species manipulate host signaling pathways in order to survive and replicate. They secrete a cocktail of up to 30 virulence factors to the cytoplasm of their eukaryotic host cells using a so-called type III secretion system. Some virulence factors belong to conserved protein families, such as the WxxxE family to which the factors SifA and SifB from Salmonella sp. belong. Most factors of this family embody bacterial GEF-mimics that activate small GTPases of the Rho family in the host. However, no such activity was found for SifA and SifB. The aim of this study was to identify new molecular targets of these WxxxE effector proteins and characterize their interaction with signaling pathways in the host.In this study, I analyzed a number of promising candidates from a previous Y2H screen (Cep70/SifA, DYNLRB1/SifA, CLIP4/SifB) using biochemical and microscopic techniques. The main part of this work focuses on the novel interaction between the Salmonella WxxxE effector protein SifB and the host factor CLIP4, an as yet uncharacterized protein. The interaction surface between the two proteins was mapped to the C-terminal CAP-Gly-domains of CLIP4, which also mediate microtubule binding. SifB interacts specifically with CLIP4, but not with other members of the CAP-Gly-family such as CLIP1, -2 and -3. The data presented here suggest that CLIP4 is a microtubule binding- and bundling protein, which is found on interphase-microtubules of post-mitotic cells in selected tissues. In cultured cells the Salmonella effector protein SifB is distributed diffusely throughout the cytoplasm, but can be recruited to microtubules in the presence of CLIP4. In addition, SifB co-localizes together with CLIP4 at highly dynamic membrane tubules, so called SIFs (Salmonella-induced filaments), which are known to form along microtubules of infected cells. The data obtained here suggest that the host protein CLIP4 interacts with the bacterial virulence factor SifB, which is deposited by Salmonella at the SIF membrane and indirectly recruited to microtubules. This might contribute to anchoring of SIFs to microtubules during late stages of Salmonella infection. This is the first report of a direct interaction between a microtubule-binding host protein and a virulence factor from Salmonella." @default.
• W2785363467 created "2018-02-23" @default.
• W2785363467 creator A5022102967 @default.
• W2785363467 date "2016-01-01" @default.
• W2785363467 modified "2023-09-26" @default.
• W2785363467 title "Identification and characterization of interactions between bacterial WxxxE-virulence proteins and host cell proteins" @default.
• W2785363467 cites W1515746818 @default.
• W2785363467 cites W1571593726 @default.
• W2785363467 cites W1599576092 @default.
• W2785363467 cites W1627263949 @default.
• W2785363467 cites W1655404396 @default.
• W2785363467 cites W1846146659 @default.
• W2785363467 cites W1968789650 @default.
• W2785363467 cites W1973552999 @default.
• W2785363467 cites W1975510716 @default.
• W2785363467 cites W1975613976 @default.
• W2785363467 cites W1977100348 @default.
• W2785363467 cites W1978191478 @default.
• W2785363467 cites W1979416003 @default.
• W2785363467 cites W1983594570 @default.
• W2785363467 cites W1984457233 @default.
• W2785363467 cites W1985501881 @default.
• W2785363467 cites W1988958302 @default.
• W2785363467 cites W1989317195 @default.
• W2785363467 cites W1992523266 @default.
• W2785363467 cites W1995887685 @default.
• W2785363467 cites W1996093745 @default.
• W2785363467 cites W1997980682 @default.
• W2785363467 cites W1998082533 @default.
• W2785363467 cites W1998975377 @default.
• W2785363467 cites W2005209191 @default.
• W2785363467 cites W2007660746 @default.
• W2785363467 cites W2012611655 @default.
• W2785363467 cites W2014382524 @default.
• W2785363467 cites W2014746756 @default.
• W2785363467 cites W2015005644 @default.
• W2785363467 cites W2015272842 @default.
• W2785363467 cites W2018271515 @default.
• W2785363467 cites W2019721152 @default.
• W2785363467 cites W2021710660 @default.
• W2785363467 cites W2023654591 @default.
• W2785363467 cites W2026182957 @default.
• W2785363467 cites W2031263113 @default.
• W2785363467 cites W2031653487 @default.
• W2785363467 cites W2033380982 @default.
• W2785363467 cites W2037574782 @default.
• W2785363467 cites W2044208802 @default.
• W2785363467 cites W2046818223 @default.
• W2785363467 cites W2047453903 @default.
• W2785363467 cites W2047730219 @default.
• W2785363467 cites W2051241165 @default.
• W2785363467 cites W2056525859 @default.
• W2785363467 cites W2057290624 @default.
• W2785363467 cites W2057328004 @default.
• W2785363467 cites W2060690139 @default.
• W2785363467 cites W2061126550 @default.
• W2785363467 cites W2061822323 @default.
• W2785363467 cites W2071018921 @default.
• W2785363467 cites W2076176011 @default.
• W2785363467 cites W2080421926 @default.
• W2785363467 cites W2085628515 @default.
• W2785363467 cites W2088185441 @default.
• W2785363467 cites W2088732289 @default.
• W2785363467 cites W2088742011 @default.
• W2785363467 cites W2090096297 @default.
• W2785363467 cites W2092571964 @default.
• W2785363467 cites W2093226717 @default.
• W2785363467 cites W2100837269 @default.
• W2785363467 cites W2107062230 @default.
• W2785363467 cites W2115235932 @default.
• W2785363467 cites W2121396317 @default.
• W2785363467 cites W2123590939 @default.
• W2785363467 cites W2124232374 @default.
• W2785363467 cites W2127012355 @default.
• W2785363467 cites W2127132565 @default.
• W2785363467 cites W2128589265 @default.
• W2785363467 cites W2130782652 @default.
• W2785363467 cites W2135018996 @default.
• W2785363467 cites W2136713334 @default.
• W2785363467 cites W2137615940 @default.
• W2785363467 cites W2140915719 @default.
• W2785363467 cites W2142726051 @default.
• W2785363467 cites W2146122755 @default.
• W2785363467 cites W2148512633 @default.
• W2785363467 cites W2154968967 @default.
• W2785363467 cites W2159789689 @default.
• W2785363467 cites W2162681116 @default.
• W2785363467 cites W2163005719 @default.
• W2785363467 cites W2167016752 @default.
• W2785363467 cites W2170671189 @default.
• W2785363467 cites W2224358368 @default.
• W2785363467 cites W2237971187 @default.
• W2785363467 cites W2778660502 @default.
• W2785363467 doi "https://doi.org/10.24355/dbbs.084-201701051529-0" @default.
• W2785363467 hasPublicationYear "2016" @default.
• W2785363467 type Work @default.
• W2785363467 sameAs 2785363467 @default.
• W2785363467 citedByCount "0" @default.
• W2785363467 crossrefType "dissertation" @default.
• W2785363467 hasAuthorship W2785363467A5022102967 @default.

• next