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- W2785765041 abstract "Xylanases are group of enzymes which bring about the hydrolysis of hemicelluloses. They have stimulated great interest due to their wide industrial and biotechnological applications. In this study, the isolation, screening and selection of xlanase-producing fungi from soil of botanical garden, Obafemi Awolowo University, Ile-Ife, Nigeria were carried out. Soil samples were collected aseptically from a depth of about 3 cm in the soil and transported in sealed cellophane bags to the laboratory where fungal isolation was carried out immediately. Characterization and identification of fungal isolates were performed using standard procedures and the isolates were then screened for xylanase production, under submerged fermentation. The effect of nutritional and environmental factors on xylanase production was determined with a view to optimize enzyme production from fungi. A total of twelve filamentous fungal species were isolated from the soil sample out of which Aspergillus flavus and Trichoderma viride were selected as the most appreciable xylanase-producers. A. flavus and T. viride exhibited xylanase yields of 28.17 ± 0.43 U/mL and 30.31 ± 0.68 U/mL, respectively, using a spore concentration of 1 x 106 spores/mL, at pH 4.0, temperature of 50°C and after 144 h of incubation. Maximum xylanase production was achieved by A. flavus with the use of xylan (28.06 ± 0.49 U/mL) as sole source of carbon and peptone (27.17 ± 0.25 U/mL) as sole source of nitrogen while optimum xylanase production was achieved by T. viride with the use of sucrose (22.37 ± 0.57 U/mL) as sole source of carbon and sodium nitrate (28.56 ± 0.46 U/mL) as sole source of nitrogen. As a result of the relatively high temperature and low pH conditions of xylanase production, both fungal species have high potentials as sources of xylanases for industrial and biotechnological applications." @default.
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- W2785765041 date "2018-01-01" @default.
- W2785765041 modified "2023-09-23" @default.
- W2785765041 title "Isolation and Screening of Xylanolytic Fungi from Soil of Botanical Garden: Xylanase Production from Aspergillus flavus and Trichoderma viride" @default.
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