FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2785809034> ?p ?o ?g. }

• W2785809034 endingPage "1574" @default.
• W2785809034 startingPage "1559" @default.
• W2785809034 abstract "Comprehensive characterization of the N-glycome of a therapeutic is challenging because glycans may harbor numerous modifications (e.g., phosphorylation, sulfation, sialic acids with possible O-acetylation). The current report presents a comparison of two chromatographic platforms for the comprehensive characterization of a recombinant human erythropoietin (rhEPO) N-glycome. The two platforms include a common workflow based on 2-AB-derivatization and hydrophilic interaction chromatography (HILIC) and a native N-linked glycan workflow employing high performance anion exchange (HPAE) chromatography. Both platforms were coupled to an Orbitrap mass spectrometer, and data dependent HCD fragmentation allowed confident structural elucidation of the glycans. Each platform identified glycans not revealed by the other, and both exhibited strengths and weaknesses. The reductive amination based HILIC workflow provided better throughput and sensitivity, had good isomer resolution, and revealed the presence of O-acetylated sialic acids. However, it exhibited poor performance toward phosphorylated glycans and did not reveal the presence of sulfated glycans. Furthermore, reductive amination introduced dehydration artifacts and modified the glycosylation profile in the rhEPO glycome. Conversely, HPAE provided unbiased charge classification (sialylation levels), improved isomer resolution, and revealed multiple phosphorylated and sulfated structures, but delivered lower throughput, had artifact peaks due to epimer formation, and loss of sialic acid O-acetylation. The MS2 based identification of phosphorylated and sulfated glycans was not possible in HILIC mode due to their poor solubility caused by the high acetonitrile concentrations employed at the beginning of the gradient. After analyzing the glycome by both approaches and determining the glycans present, a glycan library was created for site specific glycopeptide analyses. Glycopeptide analyses confirmed all the compositions annotated by the combined use of 2-AB- and native glycan workflows and provided site specific location of the glycans. These two platforms were complementary and in combination delivered a more thorough and comprehensive characterization of the rhEPO N-glycome, supporting regulatory conformance for the pharmaceutical industry." @default.
• W2785809034 created "2018-02-23" @default.
• W2785809034 creator A5003090101 @default.
• W2785809034 creator A5009811650 @default.
• W2785809034 creator A5011805101 @default.
• W2785809034 creator A5012816472 @default.
• W2785809034 creator A5027465075 @default.
• W2785809034 creator A5030032449 @default.
• W2785809034 creator A5031702923 @default.
• W2785809034 creator A5039319403 @default.
• W2785809034 creator A5041673655 @default.
• W2785809034 creator A5066009283 @default.
• W2785809034 creator A5082586608 @default.
• W2785809034 creator A5084798982 @default.
• W2785809034 creator A5088190129 @default.
• W2785809034 date "2018-02-16" @default.
• W2785809034 modified "2023-10-18" @default.
• W2785809034 title "High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin" @default.
• W2785809034 cites W1224253055 @default.
• W2785809034 cites W1553087448 @default.
• W2785809034 cites W1570484367 @default.
• W2785809034 cites W1964051802 @default.
• W2785809034 cites W1966025897 @default.
• W2785809034 cites W1966619755 @default.
• W2785809034 cites W1967961992 @default.
• W2785809034 cites W1968014420 @default.
• W2785809034 cites W1975340064 @default.
• W2785809034 cites W1978124145 @default.
• W2785809034 cites W1979353615 @default.
• W2785809034 cites W1979379256 @default.
• W2785809034 cites W1982769124 @default.
• W2785809034 cites W1987565277 @default.
• W2785809034 cites W2001607896 @default.
• W2785809034 cites W2011540538 @default.
• W2785809034 cites W2011991880 @default.
• W2785809034 cites W2013172079 @default.
• W2785809034 cites W2014634027 @default.
• W2785809034 cites W2018448767 @default.
• W2785809034 cites W2021989115 @default.
• W2785809034 cites W2027116733 @default.
• W2785809034 cites W2034959888 @default.
• W2785809034 cites W2037542029 @default.
• W2785809034 cites W2049324939 @default.
• W2785809034 cites W2054577224 @default.
• W2785809034 cites W2055184396 @default.
• W2785809034 cites W2059807960 @default.
• W2785809034 cites W2074688749 @default.
• W2785809034 cites W2075267760 @default.
• W2785809034 cites W2076709027 @default.
• W2785809034 cites W2081635088 @default.
• W2785809034 cites W2082701140 @default.
• W2785809034 cites W2085953693 @default.
• W2785809034 cites W2099980678 @default.
• W2785809034 cites W2105379208 @default.
• W2785809034 cites W2136482421 @default.
• W2785809034 cites W2141000901 @default.
• W2785809034 cites W2159768475 @default.
• W2785809034 cites W2168470451 @default.
• W2785809034 cites W2294527235 @default.
• W2785809034 cites W2330451514 @default.
• W2785809034 cites W2492346507 @default.
• W2785809034 cites W2593716310 @default.
• W2785809034 cites W2614342797 @default.
• W2785809034 cites W2725695211 @default.
• W2785809034 cites W86688592 @default.
• W2785809034 cites W952517265 @default.
• W2785809034 doi "https://doi.org/10.1021/acs.jproteome.7b00862" @default.
• W2785809034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29451981" @default.
• W2785809034 hasPublicationYear "2018" @default.
• W2785809034 type Work @default.
• W2785809034 sameAs 2785809034 @default.
• W2785809034 citedByCount "31" @default.
• W2785809034 countsByYear W27858090342018 @default.
• W2785809034 countsByYear W27858090342019 @default.
• W2785809034 countsByYear W27858090342020 @default.
• W2785809034 countsByYear W27858090342021 @default.
• W2785809034 countsByYear W27858090342022 @default.
• W2785809034 countsByYear W27858090342023 @default.
• W2785809034 crossrefType "journal-article" @default.
• W2785809034 hasAuthorship W2785809034A5003090101 @default.
• W2785809034 hasAuthorship W2785809034A5009811650 @default.
• W2785809034 hasAuthorship W2785809034A5011805101 @default.
• W2785809034 hasAuthorship W2785809034A5012816472 @default.
• W2785809034 hasAuthorship W2785809034A5027465075 @default.
• W2785809034 hasAuthorship W2785809034A5030032449 @default.
• W2785809034 hasAuthorship W2785809034A5031702923 @default.
• W2785809034 hasAuthorship W2785809034A5039319403 @default.
• W2785809034 hasAuthorship W2785809034A5041673655 @default.
• W2785809034 hasAuthorship W2785809034A5066009283 @default.
• W2785809034 hasAuthorship W2785809034A5082586608 @default.
• W2785809034 hasAuthorship W2785809034A5084798982 @default.
• W2785809034 hasAuthorship W2785809034A5088190129 @default.
• W2785809034 hasConcept C106686826 @default.
• W2785809034 hasConcept C108625454 @default.
• W2785809034 hasConcept C116817701 @default.
• W2785809034 hasConcept C155138218 @default.
• W2785809034 hasConcept C158650290 @default.
• W2785809034 hasConcept C161790260 @default.

• next