FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2786004175> ?p ?o ?g. }

• W2786004175 endingPage "232" @default.
• W2786004175 startingPage "228" @default.
• W2786004175 abstract "The proliferation of successful, cell-free reconstitutions of cytoskeletal networks have prompted measurements of forces between network elements via induced osmotic pressure by the addition of depletants. Indeed, it was through osmotic pressurization that Tau, an intrinsically disordered protein found in neuronal axons, was recently discovered to mediate two distinct microtubule (MT) bundle states, one widely spaced and a second tightly packed, separated by an energy barrier due to polyelectrolyte repulsions between opposing Tau projection domains on neighboring MT surfaces. Here, we compare interfilament force measurements in Tau coated MT bundles using PEO20k (poly(ethylene oxide), Mw = 20000), a commonly used inert depletant, and recently published measurements with PEO102k. While force measurements with either depletant reveals the transition between the two bundled states, measurements with PEO20k cannot recapitulate the correct critical pressure (Pc) at which widely spaced MT bundles transition to tightly packed MT bundles due to depletant penetration into widely spaced bundles below Pc. Surprisingly, upon transitioning to the tightly packed bundle state data from both depletants are in quantitative agreement indicative of expulsion of the smaller PEO20k depletant, but only at distances comparable or less than the PEO20k radius of gyration, significantly smaller than the effective diameter of PEO20k. While PEO102k (with size larger than the wall-to-wall distance between MTs in bundles) can more accurately capture the force response behavior at low to intermediate pressures (<104 Pa), measurements with PEO20k, beyond the overlap regime with PEO102k, extend the achievable osmotic pressure range into the higher-pressure regime (∼5 × 104 Pa). The data underscores the importance of the use of polymeric depletants of different sizes to elucidate force response behavior of cytoskeletal filamentous networks over a more complete extended pressure range." @default.
• W2786004175 created "2018-02-23" @default.
• W2786004175 creator A5006699574 @default.
• W2786004175 creator A5026538141 @default.
• W2786004175 creator A5032956501 @default.
• W2786004175 creator A5039595355 @default.
• W2786004175 creator A5041838834 @default.
• W2786004175 creator A5072682355 @default.
• W2786004175 creator A5081714411 @default.
• W2786004175 creator A5082765578 @default.
• W2786004175 creator A5087083984 @default.
• W2786004175 date "2018-02-01" @default.
• W2786004175 modified "2023-10-18" @default.
• W2786004175 title "Comparison between 102k and 20k Poly(ethylene oxide) Depletants in Osmotic Pressure Measurements of Interfilament Forces in Cytoskeletal Systems" @default.
• W2786004175 cites W2022051913 @default.
• W2786004175 cites W2026582856 @default.
• W2786004175 cites W2026636207 @default.
• W2786004175 cites W2034202927 @default.
• W2786004175 cites W2039270622 @default.
• W2786004175 cites W2046006885 @default.
• W2786004175 cites W2046624741 @default.
• W2786004175 cites W2074422628 @default.
• W2786004175 cites W2108942635 @default.
• W2786004175 cites W2126747417 @default.
• W2786004175 cites W2142635402 @default.
• W2786004175 cites W2146085202 @default.
• W2786004175 cites W2149122765 @default.
• W2786004175 cites W2154588724 @default.
• W2786004175 cites W2485106826 @default.
• W2786004175 cites W2517262294 @default.
• W2786004175 cites W2519421676 @default.
• W2786004175 doi "https://doi.org/10.1021/acsmacrolett.7b00937" @default.
• W2786004175 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35610898" @default.
• W2786004175 hasPublicationYear "2018" @default.
• W2786004175 type Work @default.
• W2786004175 sameAs 2786004175 @default.
• W2786004175 citedByCount "3" @default.
• W2786004175 countsByYear W27860041752019 @default.
• W2786004175 countsByYear W27860041752021 @default.
• W2786004175 crossrefType "journal-article" @default.
• W2786004175 hasAuthorship W2786004175A5006699574 @default.
• W2786004175 hasAuthorship W2786004175A5026538141 @default.
• W2786004175 hasAuthorship W2786004175A5032956501 @default.
• W2786004175 hasAuthorship W2786004175A5039595355 @default.
• W2786004175 hasAuthorship W2786004175A5041838834 @default.
• W2786004175 hasAuthorship W2786004175A5072682355 @default.
• W2786004175 hasAuthorship W2786004175A5081714411 @default.
• W2786004175 hasAuthorship W2786004175A5082765578 @default.
• W2786004175 hasAuthorship W2786004175A5087083984 @default.
• W2786004175 hasConcept C12554922 @default.
• W2786004175 hasConcept C142669718 @default.
• W2786004175 hasConcept C1491633281 @default.
• W2786004175 hasConcept C15920480 @default.
• W2786004175 hasConcept C159467904 @default.
• W2786004175 hasConcept C159985019 @default.
• W2786004175 hasConcept C168568712 @default.
• W2786004175 hasConcept C171250308 @default.
• W2786004175 hasConcept C185592680 @default.
• W2786004175 hasConcept C191897082 @default.
• W2786004175 hasConcept C192562407 @default.
• W2786004175 hasConcept C2777801237 @default.
• W2786004175 hasConcept C2778134712 @default.
• W2786004175 hasConcept C2779851234 @default.
• W2786004175 hasConcept C521977710 @default.
• W2786004175 hasConcept C55493867 @default.
• W2786004175 hasConcept C86803240 @default.
• W2786004175 hasConcept C9603476 @default.
• W2786004175 hasConceptScore W2786004175C12554922 @default.
• W2786004175 hasConceptScore W2786004175C142669718 @default.
• W2786004175 hasConceptScore W2786004175C1491633281 @default.
• W2786004175 hasConceptScore W2786004175C15920480 @default.
• W2786004175 hasConceptScore W2786004175C159467904 @default.
• W2786004175 hasConceptScore W2786004175C159985019 @default.
• W2786004175 hasConceptScore W2786004175C168568712 @default.
• W2786004175 hasConceptScore W2786004175C171250308 @default.
• W2786004175 hasConceptScore W2786004175C185592680 @default.
• W2786004175 hasConceptScore W2786004175C191897082 @default.
• W2786004175 hasConceptScore W2786004175C192562407 @default.
• W2786004175 hasConceptScore W2786004175C2777801237 @default.
• W2786004175 hasConceptScore W2786004175C2778134712 @default.
• W2786004175 hasConceptScore W2786004175C2779851234 @default.
• W2786004175 hasConceptScore W2786004175C521977710 @default.
• W2786004175 hasConceptScore W2786004175C55493867 @default.
• W2786004175 hasConceptScore W2786004175C86803240 @default.
• W2786004175 hasConceptScore W2786004175C9603476 @default.
• W2786004175 hasFunder F4320322120 @default.
• W2786004175 hasFunder F4320322334 @default.
• W2786004175 hasFunder F4320337359 @default.
• W2786004175 hasFunder F4320337367 @default.
• W2786004175 hasFunder F4320337480 @default.
• W2786004175 hasIssue "2" @default.
• W2786004175 hasLocation W27860041751 @default.
• W2786004175 hasLocation W27860041752 @default.
• W2786004175 hasOpenAccess W2786004175 @default.
• W2786004175 hasPrimaryLocation W27860041751 @default.
• W2786004175 hasRelatedWork W2008179763 @default.
• W2786004175 hasRelatedWork W2028987903 @default.
• W2786004175 hasRelatedWork W2029232466 @default.

• next