Matches in SemOpenAlex for { <https://semopenalex.org/work/W2786302216> ?p ?o ?g. }
- W2786302216 endingPage "3779" @default.
- W2786302216 startingPage "3770" @default.
- W2786302216 abstract "Neutrophils migrate to sites infected by pathogenic microorganisms. This migration is regulated by neutrophil-secreted ATP, which stimulates neutrophils in an autocrine manner through purinergic receptors on the plasma membrane. Although previous studies have shown that ATP is released through channels at the plasma membrane of the neutrophil, it remains unknown whether it is also released through alternate secretory systems involving vesicular mechanisms. In this study, we investigated the possible involvement of vesicular nucleotide transporter (VNUT), a key molecule for vesicular storage and nucleotide release, in ATP secretion from neutrophils. RT-PCR and Western blotting analysis indicated that VNUT is expressed in mouse neutrophils. Immunohistochemical analysis indicated that VNUT mainly colocalized with matrix metalloproteinase-9 (MMP-9), a marker of tertiary granules, which are secretory organelles. In mouse neutrophils, ATP release was inhibited by clodronate, which is a potent VNUT inhibitor. Furthermore, neutrophils from VNUT−/− mice did not release ATP and exhibited significantly reduced migration in vitro and in vivo. These findings suggest that tertiary granule-localized VNUT is responsible for vesicular ATP release and subsequent neutrophil migration. Thus, these findings suggest an additional mechanism through which ATP is released by neutrophils. Neutrophils migrate to sites infected by pathogenic microorganisms. This migration is regulated by neutrophil-secreted ATP, which stimulates neutrophils in an autocrine manner through purinergic receptors on the plasma membrane. Although previous studies have shown that ATP is released through channels at the plasma membrane of the neutrophil, it remains unknown whether it is also released through alternate secretory systems involving vesicular mechanisms. In this study, we investigated the possible involvement of vesicular nucleotide transporter (VNUT), a key molecule for vesicular storage and nucleotide release, in ATP secretion from neutrophils. RT-PCR and Western blotting analysis indicated that VNUT is expressed in mouse neutrophils. Immunohistochemical analysis indicated that VNUT mainly colocalized with matrix metalloproteinase-9 (MMP-9), a marker of tertiary granules, which are secretory organelles. In mouse neutrophils, ATP release was inhibited by clodronate, which is a potent VNUT inhibitor. Furthermore, neutrophils from VNUT−/− mice did not release ATP and exhibited significantly reduced migration in vitro and in vivo. These findings suggest that tertiary granule-localized VNUT is responsible for vesicular ATP release and subsequent neutrophil migration. Thus, these findings suggest an additional mechanism through which ATP is released by neutrophils." @default.
- W2786302216 created "2018-02-23" @default.
- W2786302216 creator A5004964979 @default.
- W2786302216 creator A5019855418 @default.
- W2786302216 creator A5033713077 @default.
- W2786302216 creator A5035126720 @default.
- W2786302216 creator A5035881835 @default.
- W2786302216 creator A5065073841 @default.
- W2786302216 date "2018-03-01" @default.
- W2786302216 modified "2023-09-29" @default.
- W2786302216 title "Vesicular nucleotide transporter mediates ATP release and migration in neutrophils" @default.
- W2786302216 cites W1480544133 @default.
- W2786302216 cites W1673744647 @default.
- W2786302216 cites W1964988229 @default.
- W2786302216 cites W1978934458 @default.
- W2786302216 cites W1982230576 @default.
- W2786302216 cites W1987062630 @default.
- W2786302216 cites W1988110584 @default.
- W2786302216 cites W1993891203 @default.
- W2786302216 cites W1999163778 @default.
- W2786302216 cites W2005065808 @default.
- W2786302216 cites W2010239636 @default.
- W2786302216 cites W2015608635 @default.
- W2786302216 cites W2031111964 @default.
- W2786302216 cites W2034643457 @default.
- W2786302216 cites W2045362817 @default.
- W2786302216 cites W2065075422 @default.
- W2786302216 cites W2069184106 @default.
- W2786302216 cites W2079492097 @default.
- W2786302216 cites W2088029079 @default.
- W2786302216 cites W2096663326 @default.
- W2786302216 cites W2100936098 @default.
- W2786302216 cites W2112200553 @default.
- W2786302216 cites W2124011290 @default.
- W2786302216 cites W2137141750 @default.
- W2786302216 cites W2138939151 @default.
- W2786302216 cites W2139812868 @default.
- W2786302216 cites W2140995313 @default.
- W2786302216 cites W2148053686 @default.
- W2786302216 cites W2149444026 @default.
- W2786302216 cites W2151579964 @default.
- W2786302216 cites W2170992247 @default.
- W2786302216 cites W2312516986 @default.
- W2786302216 cites W2317687805 @default.
- W2786302216 cites W2341867771 @default.
- W2786302216 cites W2460148549 @default.
- W2786302216 cites W2627049514 @default.
- W2786302216 cites W2738480983 @default.
- W2786302216 cites W2770354437 @default.
- W2786302216 doi "https://doi.org/10.1074/jbc.m117.810168" @default.
- W2786302216 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5846168" @default.
- W2786302216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29363573" @default.
- W2786302216 hasPublicationYear "2018" @default.
- W2786302216 type Work @default.
- W2786302216 sameAs 2786302216 @default.
- W2786302216 citedByCount "25" @default.
- W2786302216 countsByYear W27863022162018 @default.
- W2786302216 countsByYear W27863022162019 @default.
- W2786302216 countsByYear W27863022162020 @default.
- W2786302216 countsByYear W27863022162021 @default.
- W2786302216 countsByYear W27863022162022 @default.
- W2786302216 countsByYear W27863022162023 @default.
- W2786302216 crossrefType "journal-article" @default.
- W2786302216 hasAuthorship W2786302216A5004964979 @default.
- W2786302216 hasAuthorship W2786302216A5019855418 @default.
- W2786302216 hasAuthorship W2786302216A5033713077 @default.
- W2786302216 hasAuthorship W2786302216A5035126720 @default.
- W2786302216 hasAuthorship W2786302216A5035881835 @default.
- W2786302216 hasAuthorship W2786302216A5065073841 @default.
- W2786302216 hasBestOaLocation W27863022161 @default.
- W2786302216 hasConcept C128240485 @default.
- W2786302216 hasConcept C130316041 @default.
- W2786302216 hasConcept C167019083 @default.
- W2786302216 hasConcept C170493617 @default.
- W2786302216 hasConcept C172590197 @default.
- W2786302216 hasConcept C185592680 @default.
- W2786302216 hasConcept C2778938600 @default.
- W2786302216 hasConcept C2779508331 @default.
- W2786302216 hasConcept C28406088 @default.
- W2786302216 hasConcept C41625074 @default.
- W2786302216 hasConcept C49039625 @default.
- W2786302216 hasConcept C55493867 @default.
- W2786302216 hasConcept C57306754 @default.
- W2786302216 hasConcept C67907053 @default.
- W2786302216 hasConcept C86803240 @default.
- W2786302216 hasConcept C95444343 @default.
- W2786302216 hasConceptScore W2786302216C128240485 @default.
- W2786302216 hasConceptScore W2786302216C130316041 @default.
- W2786302216 hasConceptScore W2786302216C167019083 @default.
- W2786302216 hasConceptScore W2786302216C170493617 @default.
- W2786302216 hasConceptScore W2786302216C172590197 @default.
- W2786302216 hasConceptScore W2786302216C185592680 @default.
- W2786302216 hasConceptScore W2786302216C2778938600 @default.
- W2786302216 hasConceptScore W2786302216C2779508331 @default.
- W2786302216 hasConceptScore W2786302216C28406088 @default.
- W2786302216 hasConceptScore W2786302216C41625074 @default.
- W2786302216 hasConceptScore W2786302216C49039625 @default.
- W2786302216 hasConceptScore W2786302216C55493867 @default.