Matches in SemOpenAlex for { <https://semopenalex.org/work/W2786369544> ?p ?o ?g. }
- W2786369544 endingPage "2378" @default.
- W2786369544 startingPage "2368" @default.
- W2786369544 abstract "One-dimensional heteronuclear relaxation dispersion NMR spectroscopy at 13C natural abundance successfully characterized the dynamics of the hydrogen–deuterium exchange reaction occurring at the Nε position in l-arginine by monitoring Cδ in varying amounts of D2O. A small equilibrium isotope effect was observed and quantified, corresponding to ΔG = −0.14 kcal mol–1. A bimolecular rate constant of kD = 5.1 × 109 s–1 M–1 was determined from the pH*-dependence of kex (where pH* is the direct electrode reading of pH in 10% D2O and kex is the nuclear spin exchange rate constant), consistent with diffusion-controlled kinetics. The measurement of ΔG serves to bridge the millisecond time scale lifetimes of the detectable positively charged arginine species with the nanosecond time scale lifetime of the nonobservable low-populated neutral arginine intermediate species, thus allowing for characterization of the equilibrium lifetimes of the various arginine species in solution as a function of fractional solvent deuterium content. Despite the system being in fast exchange on the chemical shift time scale, the magnitude of the secondary isotope shift due to the exchange reaction at Nε was accurately measured to be 0.12 ppm directly from curve-fitting D2O-dependent dispersion data collected at a single static field strength. These results indicate that relaxation dispersion NMR spectroscopy is a robust and general method for studying base-catalyzed hydrogen–deuterium exchange chemistry at equilibrium." @default.
- W2786369544 created "2018-02-23" @default.
- W2786369544 creator A5001505869 @default.
- W2786369544 creator A5024593759 @default.
- W2786369544 creator A5038652876 @default.
- W2786369544 creator A5058116188 @default.
- W2786369544 date "2018-02-15" @default.
- W2786369544 modified "2023-09-25" @default.
- W2786369544 title "Measurement and Characterization of Hydrogen–Deuterium Exchange Chemistry Using Relaxation Dispersion NMR Spectroscopy" @default.
- W2786369544 cites W1489827117 @default.
- W2786369544 cites W151697861 @default.
- W2786369544 cites W1576127119 @default.
- W2786369544 cites W1874093166 @default.
- W2786369544 cites W189045671 @default.
- W2786369544 cites W1967820830 @default.
- W2786369544 cites W1969876717 @default.
- W2786369544 cites W1972060641 @default.
- W2786369544 cites W1977179882 @default.
- W2786369544 cites W1981596913 @default.
- W2786369544 cites W1982013103 @default.
- W2786369544 cites W1984068166 @default.
- W2786369544 cites W1987528091 @default.
- W2786369544 cites W198782672 @default.
- W2786369544 cites W1989574807 @default.
- W2786369544 cites W1990126142 @default.
- W2786369544 cites W1993533697 @default.
- W2786369544 cites W1994577377 @default.
- W2786369544 cites W1994939140 @default.
- W2786369544 cites W1995447876 @default.
- W2786369544 cites W1996229842 @default.
- W2786369544 cites W1999187807 @default.
- W2786369544 cites W2004252276 @default.
- W2786369544 cites W2005412294 @default.
- W2786369544 cites W2009698511 @default.
- W2786369544 cites W2010182484 @default.
- W2786369544 cites W2011881868 @default.
- W2786369544 cites W2012316183 @default.
- W2786369544 cites W2012438313 @default.
- W2786369544 cites W2014100705 @default.
- W2786369544 cites W2015282297 @default.
- W2786369544 cites W2016367377 @default.
- W2786369544 cites W2018012356 @default.
- W2786369544 cites W2018736034 @default.
- W2786369544 cites W2019210215 @default.
- W2786369544 cites W2027685431 @default.
- W2786369544 cites W2028478523 @default.
- W2786369544 cites W2031628248 @default.
- W2786369544 cites W2033269344 @default.
- W2786369544 cites W2035338880 @default.
- W2786369544 cites W2042478996 @default.
- W2786369544 cites W2046002103 @default.
- W2786369544 cites W2048322679 @default.
- W2786369544 cites W2050785572 @default.
- W2786369544 cites W2052437133 @default.
- W2786369544 cites W2053850848 @default.
- W2786369544 cites W2056229694 @default.
- W2786369544 cites W2058708926 @default.
- W2786369544 cites W2062972508 @default.
- W2786369544 cites W2066558081 @default.
- W2786369544 cites W2068227670 @default.
- W2786369544 cites W2068534654 @default.
- W2786369544 cites W2069106100 @default.
- W2786369544 cites W2076283593 @default.
- W2786369544 cites W2077478723 @default.
- W2786369544 cites W2092473163 @default.
- W2786369544 cites W2094460693 @default.
- W2786369544 cites W2103220747 @default.
- W2786369544 cites W2107573334 @default.
- W2786369544 cites W2107949693 @default.
- W2786369544 cites W2114214187 @default.
- W2786369544 cites W2118493964 @default.
- W2786369544 cites W2119320884 @default.
- W2786369544 cites W2124929240 @default.
- W2786369544 cites W2127619077 @default.
- W2786369544 cites W2131625617 @default.
- W2786369544 cites W2137630006 @default.
- W2786369544 cites W2141218040 @default.
- W2786369544 cites W2141980994 @default.
- W2786369544 cites W2143603753 @default.
- W2786369544 cites W2147106126 @default.
- W2786369544 cites W2164155150 @default.
- W2786369544 cites W2165849976 @default.
- W2786369544 cites W2170036411 @default.
- W2786369544 cites W2293958709 @default.
- W2786369544 cites W2317669940 @default.
- W2786369544 cites W2318300059 @default.
- W2786369544 cites W2326344583 @default.
- W2786369544 cites W2328316876 @default.
- W2786369544 cites W2402445610 @default.
- W2786369544 cites W2419139792 @default.
- W2786369544 cites W2551599160 @default.
- W2786369544 cites W30334872 @default.
- W2786369544 cites W382540865 @default.
- W2786369544 cites W4250873843 @default.
- W2786369544 doi "https://doi.org/10.1021/acs.jpcb.7b10849" @default.
- W2786369544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29376350" @default.
- W2786369544 hasPublicationYear "2018" @default.
- W2786369544 type Work @default.