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• W2786407315 abstract "In the first part of this work, the mechanisms involved in plasmid transfer from the intracellular bacterium L. monocytogenes to eukaryotic host cells were investigated. It could be shown that the initial invasion of the host cell is not limiting for efficient gene transfer. Also, bacteria are efficiently lysed upon antibiotic treatment and release their content to the cytosol, but only a fraction of the plasmids is eventually transferred to the nucleus. Released plasmids are probably associated with high molecular weight components which might limit nuclear transfer. Also, a small amount of bacterial chromosomal DNA was transferred to the nucleus and integrated in the host cell genome. In the second part of this work, L. monocytogenes and S. flexneri were tested as gene transfer vehicles for cancer therapy. The dissemination of both bacteria was analyzed after infection via the intratumoural route. The bacteria were not contained within the tumour tissue but were also found in liver and spleen of mice. When mice were systemically infected with attenuated S. flexneri, the bacteria were rapidly cleared from the blood stream and did not accumulate in tumours or organs, as described before for other bacterial species. Gene transfer from bacteria to tumour cells in vivo could not clearly be demonstrated for Listeria under the conditions used. In contrast, some implication of gene transfer by Shigella was found. Here, refinement of the technical settings should allow the definite detection of even low levels of transgene expression. In summary, extending the present work will result in better gene transfer abilities and will reveal the great potential of such bacteria as DNA carriers." @default.
• W2786407315 created "2018-02-23" @default.
• W2786407315 creator A5052900839 @default.
• W2786407315 date "2005-04-13" @default.
• W2786407315 modified "2023-09-24" @default.
• W2786407315 title "Intracellular bacteria as DNA carriers in vitro and in vivo" @default.
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