FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2786436646> ?p ?o ?g. }

• W2786436646 abstract "The present study investigated the effects of dual specificity phosphatase 1 (DUSP1) gene silencing using lentiviral vector-mediated small interfering (si)RNA on the release of proinflammatory cytokines through the regulation of the mitogen‑activated protein kinase (MAPK) signaling pathway in mice with acute pancreatitis (AP). Two siRNA‑DUSP1 sequences and one scramble siRNA sequence were designed, and the expression of DUSP1 was detected using western blot analysis to screen for the one with a higher interference rate. An AP mouse model was established, and KM mice were assigned to either a control, siRNA, AP, AP+PD98059, AP+scramble, AP+siRNA or AP+PD98059+siRNA group. The expression of proinflammatory cytokines, including tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6, high mobility group box 1 (HMGB1), and S100A12 in serum samples were detected using an enzyme‑linked immunosorbent assay at 12, 24 and 48 h post‑modeling. The serum amylase levels were also detected. The expression levels of DUSP1, TNF‑α, IL‑1β, IL‑6, HMGB1, S100A12, phosphorylated (p‑) extracellular signal‑regulated kinase (ERK), p‑c‑Jun N‑terminal kinase (JNK), p‑p38, ERK, JNK and p38 in pancreatic, liver, kidney and lung tissues were detected using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Compared with the control group, the siRNA group demonstrated marginally upregulated serum amylase, lipase, urinary trypsinogen‑2, and proinflammatory cytokines, HMGB1 and S100A12 in serum and tissues, with no statistically significant difference, elevated expression levels of p‑ERK, p‑JNK and p‑p38, and decreased expression of DUSP1. The other five groups demonstrated increased expression levels of TNF‑α, IL‑1β, IL‑6, HMGB1, S100A12, amylase, lipase and urinary trypsinogen‑2 in serum, and increased expression levels of DUSP1, TNF‑α, IL‑1β, IL‑6, HMGB1, S100A12, p‑ERK, p‑JNK and p‑p38 in tissues. Compared with the AP group, the AP+PD98059+siRNA group had decreased expression of DUSP1 in tissues, whereas the AP+PD98059 group had decreased serum expression levels of TNF‑α, IL‑1β, IL‑6, HMGB1, S100A12 and amylase, lipase and urinary trypsinogen‑2. The expression levels of TNF‑α, IL‑1β, IL‑6, HMGB1, S100A12, p‑ERK, p‑JNK, p‑p38 in tissues, and edema of pancreatic tissue were alleviated, whereas the opposite results were observed in the AP+siRNA group with the decreased expression of DUSP1. The results suggested that DUSP1 gene silencing promoted the release of proinflammatory cytokines through activation of the MAPK signaling pathway in mice with AP." @default.
• W2786436646 created "2018-02-23" @default.
• W2786436646 creator A5006076804 @default.
• W2786436646 creator A5011877804 @default.
• W2786436646 creator A5027332725 @default.
• W2786436646 creator A5070059174 @default.
• W2786436646 creator A5077976040 @default.
• W2786436646 creator A5081362397 @default.
• W2786436646 creator A5082047549 @default.
• W2786436646 date "2018-01-25" @default.
• W2786436646 modified "2023-10-16" @default.
• W2786436646 title "Effects of silencing the DUSP1 gene using lentiviral vector-mediated siRNA on the release of proinflammatory cytokines through regulation of the MAPK signaling pathway in mice with acute pancreatitis" @default.
• W2786436646 cites W1502545809 @default.
• W2786436646 cites W1710968639 @default.
• W2786436646 cites W1979020933 @default.
• W2786436646 cites W1981080717 @default.
• W2786436646 cites W1981712836 @default.
• W2786436646 cites W1991668507 @default.
• W2786436646 cites W2011420458 @default.
• W2786436646 cites W2023354489 @default.
• W2786436646 cites W2025862326 @default.
• W2786436646 cites W2028556625 @default.
• W2786436646 cites W2033549736 @default.
• W2786436646 cites W2036374414 @default.
• W2786436646 cites W2038484424 @default.
• W2786436646 cites W2041769117 @default.
• W2786436646 cites W2054586162 @default.
• W2786436646 cites W2061594475 @default.
• W2786436646 cites W2075995473 @default.
• W2786436646 cites W2083702396 @default.
• W2786436646 cites W2084204348 @default.
• W2786436646 cites W2087863662 @default.
• W2786436646 cites W2099769485 @default.
• W2786436646 cites W2107277218 @default.
• W2786436646 cites W2107495112 @default.
• W2786436646 cites W2132731589 @default.
• W2786436646 cites W2143400244 @default.
• W2786436646 cites W2163784462 @default.
• W2786436646 cites W2166847341 @default.
• W2786436646 cites W2225975946 @default.
• W2786436646 cites W2233343952 @default.
• W2786436646 cites W2337589896 @default.
• W2786436646 cites W2557917628 @default.
• W2786436646 cites W2891220544 @default.
• W2786436646 cites W3189971259 @default.
• W2786436646 cites W344235072 @default.
• W2786436646 cites W4230469392 @default.
• W2786436646 doi "https://doi.org/10.3892/ijmm.2018.3429" @default.
• W2786436646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29393354" @default.
• W2786436646 hasPublicationYear "2018" @default.
• W2786436646 type Work @default.
• W2786436646 sameAs 2786436646 @default.
• W2786436646 citedByCount "6" @default.
• W2786436646 countsByYear W27864366462019 @default.
• W2786436646 countsByYear W27864366462020 @default.
• W2786436646 countsByYear W27864366462022 @default.
• W2786436646 countsByYear W27864366462023 @default.
• W2786436646 crossrefType "journal-article" @default.
• W2786436646 hasAuthorship W2786436646A5006076804 @default.
• W2786436646 hasAuthorship W2786436646A5011877804 @default.
• W2786436646 hasAuthorship W2786436646A5027332725 @default.
• W2786436646 hasAuthorship W2786436646A5070059174 @default.
• W2786436646 hasAuthorship W2786436646A5077976040 @default.
• W2786436646 hasAuthorship W2786436646A5081362397 @default.
• W2786436646 hasAuthorship W2786436646A5082047549 @default.
• W2786436646 hasBestOaLocation W27864366461 @default.
• W2786436646 hasConcept C104317684 @default.
• W2786436646 hasConcept C119056186 @default.
• W2786436646 hasConcept C153911025 @default.
• W2786436646 hasConcept C164027704 @default.
• W2786436646 hasConcept C184235292 @default.
• W2786436646 hasConcept C203014093 @default.
• W2786436646 hasConcept C22615655 @default.
• W2786436646 hasConcept C2776914184 @default.
• W2786436646 hasConcept C51551487 @default.
• W2786436646 hasConcept C54009773 @default.
• W2786436646 hasConcept C54355233 @default.
• W2786436646 hasConcept C55493867 @default.
• W2786436646 hasConcept C57074206 @default.
• W2786436646 hasConcept C81885089 @default.
• W2786436646 hasConcept C86803240 @default.
• W2786436646 hasConcept C95444343 @default.
• W2786436646 hasConceptScore W2786436646C104317684 @default.
• W2786436646 hasConceptScore W2786436646C119056186 @default.
• W2786436646 hasConceptScore W2786436646C153911025 @default.
• W2786436646 hasConceptScore W2786436646C164027704 @default.
• W2786436646 hasConceptScore W2786436646C184235292 @default.
• W2786436646 hasConceptScore W2786436646C203014093 @default.
• W2786436646 hasConceptScore W2786436646C22615655 @default.
• W2786436646 hasConceptScore W2786436646C2776914184 @default.
• W2786436646 hasConceptScore W2786436646C51551487 @default.
• W2786436646 hasConceptScore W2786436646C54009773 @default.
• W2786436646 hasConceptScore W2786436646C54355233 @default.
• W2786436646 hasConceptScore W2786436646C55493867 @default.
• W2786436646 hasConceptScore W2786436646C57074206 @default.
• W2786436646 hasConceptScore W2786436646C81885089 @default.
• W2786436646 hasConceptScore W2786436646C86803240 @default.
• W2786436646 hasConceptScore W2786436646C95444343 @default.
• W2786436646 hasLocation W27864366461 @default.
• W2786436646 hasLocation W27864366462 @default.

• next