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• W2786577812 abstract "Detection, inhibition and molecular analysis of multidrug resistant aerobic Gram-negative clinical isolates from a tertiary hospital in NigeriaO. ADELUOLA 1, 3, K. S. OYEDEJI 2 , U. E. MENDIE 1 , J.R. JOHNSON3 AND J.R. PORTER3 Dept. of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Lagos. NigeriaDept. of Medical Laboratory Science, College of Medicine, University of Lagos. Nigeria.Dept. Of Biological Sciences, Misher College of Arts and Science, University of the Sciences in Philadelphia. USA.Corresponding author:Adebowale Olufemi Adeluola B.Pharm., PhDDepartment of Pharmaceutics and Pharmaceutical Technology,University of Lagos, Nigeria.Phone: +2347056004859; E-mail address: debodelu@gmail.com; aadeluola@unilag.edu.ng ABSTRACT The challenge of combating the ever emerging multi-drug resistant (MDR) clinical isolates in the face of a slow rate of discovery of new classes of antibiotics is a problem in antibiotic chemotherapy. This study was aimed at (i) linking phenotypic antibiotic drug-resistance characteristics detected in randomly-sampled clinical isolates with detectable genetic markers. (ii) screening a suspected efflux pump inhibitor (EPI) [1-(3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could be helpful in combating this challenge.Fifty-one isolates; 28 Klebsiella pneumonia, 3 E. Coli, 1 Enterobacter cloacae, 1 E. aerogenes, 5 Proteus mirabilis, 4 Providencia rettgeri, 1 P. stuartii, 1 Serratia liquefaciens, 6 S. odorifera, and 1 Acinetobacter baumannii obtained from infections of urinary tract, upper respiratory tract, gastrointestinal tract, ear swab, eye swab, and blood culture were screened for (i) antibiotic-susceptibility over a range of 11 classes of antibiotics, (ii) β-lactamase production, (iii) ESBL production and (iv) Efflux pump activity (EPA) in the presence and absence of 1-[3-(trifluoromethyl) benzyl]-piperazine (TFMBP). Molecular analysis was done using DNA extraction by boiling and the randomly-amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) procedure with 2% agarose gel electrophoresis stained with ethidium bromide at 10 µg/ml and visualized by UV trans-illumination.AmpC β-lactamase (4%) and K1 β-lactamase (5.8%) were detected with no carbapenemase producers. AcrA and AcrB marker genes were detected in 12% of the isolates while blaCTX-M (8%) and blaTEM (4%) were also detected.Antibiotic resistance due to EPA can be combated with a suitable EPI as demonstrated by TFMBP when combined with specific antibiotics.Keywords: TFMBP; Efflux Pump Activity; ESBL; MDR; Carbapenemase Running Title: Inhibition and molecular analysis of multidrug resistance with Efflux pump activity." @default.
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• W2786577812 date "2018-01-05" @default.
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• W2786577812 title "Detection, inhibition and molecular analysis of multidrug resistant aerobic Gram-negative clinical isolates from a tertiary hospital in Nigeria" @default.
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